ID   SRB8_COCIM              Reviewed;        1554 AA.
AC   Q1EAK2; J3KGZ4;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 12;
DE   AltName: Full=Mediator complex subunit 12;
GN   Name=SRB8; Synonyms=MED12; ORFNames=CIMG_00411;
OS   Coccidioides immitis (strain RS) (Valley fever fungus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Onygenales; Onygenaceae; Coccidioides.
OX   NCBI_TaxID=246410;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RS;
RX   PubMed=19717792; DOI=10.1101/gr.087551.108;
RA   Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R.,
RA   Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y.,
RA   McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M.,
RA   Barker B.M., Galgiani J.N., Orbach M.J., Kirkland T.N., Cole G.T.,
RA   Henn M.R., Birren B.W., Taylor J.W.;
RT   "Comparative genomic analyses of the human fungal pathogens Coccidioides
RT   and their relatives.";
RL   Genome Res. 19:1722-1731(2009).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=RS;
RX   PubMed=20516208; DOI=10.1101/gr.103911.109;
RA   Neafsey D.E., Barker B.M., Sharpton T.J., Stajich J.E., Park D.J.,
RA   Whiston E., Hung C.-Y., McMahan C., White J., Sykes S., Heiman D.,
RA   Young S., Zeng Q., Abouelleil A., Aftuck L., Bessette D., Brown A.,
RA   FitzGerald M., Lui A., Macdonald J.P., Priest M., Orbach M.J.,
RA   Galgiani J.N., Kirkland T.N., Cole G.T., Birren B.W., Henn M.R.,
RA   Taylor J.W., Rounsley S.D.;
RT   "Population genomic sequencing of Coccidioides fungi reveals recent
RT   hybridization and transposon control.";
RL   Genome Res. 20:938-946(2010).
CC   -!- FUNCTION: Component of the SRB8-11 complex. The SRB8-11 complex is a
CC       regulatory module of the Mediator complex which is itself involved in
CC       regulation of basal and activated RNA polymerase II-dependent
CC       transcription. The SRB8-11 complex may be involved in the
CC       transcriptional repression of a subset of genes regulated by Mediator.
CC       It may inhibit the association of the Mediator complex with RNA
CC       polymerase II to form the holoenzyme complex (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of the SRB8-11 complex, which itself associates with
CC       the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC       {ECO:0000305}.
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DR   EMBL; GG704911; EAS35057.3; -; Genomic_DNA.
DR   RefSeq; XP_001246640.2; XM_001246639.2.
DR   AlphaFoldDB; Q1EAK2; -.
DR   STRING; 246410.Q1EAK2; -.
DR   EnsemblFungi; EAS35057; EAS35057; CIMG_00411.
DR   GeneID; 4566041; -.
DR   KEGG; cim:CIMG_00411; -.
DR   VEuPathDB; FungiDB:CIMG_00411; -.
DR   InParanoid; Q1EAK2; -.
DR   OMA; DNKREAW; -.
DR   OrthoDB; 285540at2759; -.
DR   Proteomes; UP000001261; Unassembled WGS sequence.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR019035; Mediator_Med12.
DR   Pfam; PF09497; Med12; 1.
DR   SMART; SM01281; Med12; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1554
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   12"
FT                   /id="PRO_0000312970"
FT   REGION          1..73
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          128..173
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..64
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1554 AA;  173384 MW;  6AECEE47B5A10735 CRC64;
     MASLNTPECP VMSSRPSMSC GNSSSSGLAI QGHSQRPSQT DPSAVEQAAQ DSVIDLTNSD
     YETCDANRAT KRQKLDLRID VPNTQRAPKA RFSQAVSSPF ALQDTVPPIR TGRPFWDFGE
     EVSGFGFQGS LESEIPNDEP EQPDSLPPLP VRPWRYRPQG HSSREETPRV NQNAGAEVQT
     TPFCIEVPEA APIFDSGKPL DFYPWKGDHP EDSLTDQTAK QGYYDRVQVS QNESNTARPS
     LYVQLKHRSG LKLLSSVFTA AIDKRQSHCR VTGFSTFKPP PRVTLTDIKR ETWLRDLANP
     SVPLRRLSRT IPHGIRGRIL LDQCVNKAIP IGRAVWLVKC VGANEIRAFK RKGTSAAITH
     SLEVKWVRDW SINVHQFIES VVTACGTPNW ASTMSYVIRF AARLFQEQLL DQALCLDWFL
     QSLRTAPIGT LPIWLSMLGA YWTSLVRYRK RGRPLAESLL EKLKTILSLE DSGPKKQIAD
     RLSWLIKTFA ESHPACFVLP LTWRTYKSTL TTCFCLHLSE DNRKIGALSN RNERIVKAEI
     CRQSGRQSPS QQVICLLDSA DSLNDMEILS SGCLNLHFDH GSLIMKILEW VSTSFRRGSA
     RVYVAVRLLR KWRRAGMDTD NCIVNFLQQK ADTPGFSPAN IYHLVCELVR SQSFAVGKYL
     QWLMARGAVR NDAPMHPTVQ LLAHLPRRKL PSHVWNLRNT LLTKAGFLLS SENQQILNTK
     RYIHHRLPEV ITKPIAGDNE GLFTPSDLSN LNWTIKSEIG HWIREHVSLH RKRYLRTAPN
     HHNACAIEIS ALTPAQFFEI RDIIECLGDL SLLADILKHT SSSDNIIVLI SAVDTLNYCA
     DSFKAIGALS DLFKSALAGY AHVNKADVSI IELISSLLEV GMKLPGEIPV VAMLRRDLSH
     FDKKFTGAVS SPVSDHTGES VNIASPAFSE GLHQLLNSGS GMDEPNMNKI FDMLSRKLKQ
     SKEPNASSHE IARHLSQLRV LNSDVFDRLM VKWIISTLKS SPRPHLLTFL PPLIGVGCVT
     FEAFFALIDR LLKSDSHRQS IQDVSGLRYE MINLLRKEIF YELGSIDLVS YRFKTTREEY
     ITHHAYDALG LVNEALANVN PESTSNPPLV PLLCELIIQN LNAFGPEGAG RIVEEFPNCV
     DIIHQALDVL LGLKAQVGVQ KTQSVVGLID DLSLSYCLVK LRLLLDANPD GDAARSSIVD
     LLFRTAESDI RNGERRWLEV LNVLPVSAAH LIRQRAEREI LSLSLISSSL TTFSSEEDAL
     IYLCIVEELS YSIPDEFSPS SMGADLGDKM FLLLQKTVEL ANAKKGTDLE SGASSTALGV
     AELSIGVWFF VLLRLVALHR SLVPPNCDSR TEINHQTRLL VQICCISRSP LFARRSTQSF
     ISSISTFTKC DSLLRMLPSS WANLQLQALD VCSTLVDTLS DEARYECARF LRDKCPAFLH
     PQNDARLLFL FGPIIESQSI TTHGSSRAST STPVTNLTQH VQSTPPPSQN TLTPGMVEEP
     NLFTNKLRFQ QNGRITGPYP PKPWEMLGDA APIIGINDTP VNLAYFGTRQ SKRL