ID   SRB8_KLULA              Reviewed;        1322 AA.
AC   Q6CKZ5;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 12;
DE   AltName: Full=Mediator complex subunit 12;
GN   Name=SRB8; Synonyms=MED12; OrderedLocusNames=KLLA0F06919g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the SRB8-11 complex. The SRB8-11 complex is a
CC       regulatory module of the Mediator complex which is itself involved in
CC       regulation of basal and activated RNA polymerase II-dependent
CC       transcription. The SRB8-11 complex may be involved in the
CC       transcriptional repression of a subset of genes regulated by Mediator.
CC       It may inhibit the association of the Mediator complex with RNA
CC       polymerase II to form the holoenzyme complex (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of the SRB8-11 complex, which itself associates with
CC       the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC       {ECO:0000305}.
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DR   EMBL; CR382126; CAG98102.1; -; Genomic_DNA.
DR   RefSeq; XP_455394.1; XM_455394.1.
DR   AlphaFoldDB; Q6CKZ5; -.
DR   SMR; Q6CKZ5; -.
DR   STRING; 28985.XP_455394.1; -.
DR   EnsemblFungi; CAG98102; CAG98102; KLLA0_F06919g.
DR   GeneID; 2895684; -.
DR   KEGG; kla:KLLA0_F06919g; -.
DR   eggNOG; KOG4522; Eukaryota.
DR   HOGENOM; CLU_256280_0_0_1; -.
DR   InParanoid; Q6CKZ5; -.
DR   OMA; EFIVYHQ; -.
DR   Proteomes; UP000000598; Chromosome F.
DR   GO; GO:1990508; C:CKM complex; IEA:EnsemblFungi.
DR   GO; GO:0016592; C:mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0003713; F:transcription coactivator activity; IEA:EnsemblFungi.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   GO; GO:0000411; P:positive regulation of transcription by galactose; IEA:EnsemblFungi.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   GO; GO:0006468; P:protein phosphorylation; IEA:EnsemblFungi.
DR   InterPro; IPR019035; Mediator_Med12.
DR   Pfam; PF09497; Med12; 1.
DR   SMART; SM01281; Med12; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1322
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   12"
FT                   /id="PRO_0000312973"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1322 AA;  154880 MW;  67D509F61F4557FF CRC64;
     MSPSKYLLTP PEELHPLTDS NPQIYPDFDP WIHTKEEDKI LKEFVAKGYY TASRVNFETI
     SARSALQGSL PKVSSLLGDE LSKIIEIREQ EINRISTLDI EDSTRFTKWS GQDFHLPNRV
     TLTDQKRNLW LQELSSSNTS LRKLTKSVPH GFRKRHILEQ CYTQFVPIYR VIWLIKSCYA
     IEWKGMISKA KGETTHEELL STLYKDWTDN MVLILEKLVF EVSKYYNDPG QLKYWKLRIA
     HYLKILGNCY EMELLNRSTL HQWLVDFLSK IESFEFYPMT LHILSIFWSG ILGTTEEDTE
     IESSFLIIKL SEILLRKYHI ISESKCMIND SKYIINDVQR NSKLKESVLL RLRQFISHIF
     HTQSLEAFIM PKQNWNIYKN YLYQILLQNV SDEETPKLKK KLKLISYRNE SLRLGSLDVE
     PSRETSPFST ELTLDNVFSG NILNLKNVSP ELLGVLDSGL SGSEWSLFID QKITKMEQVI
     EIILWAINPS RKHRYDSCHL VAKILVLKIN SQESFQEYSI EDVIWSLIFH FSKLSKLELQ
     KIVWLPKLHQ LLNVFIGYGI IKVPTYIRRL ISSGVLYLPE SENKNFHCKL LINLKISPIM
     KSQYNMVLKN VMEYAPHFYE KYNFDKLVSI FESLKPKLLS GDFERLEEYP GSIRIMCSEW
     YLSQICFNQG ELQKVNNHKI IKTFQLFCIN LGELHHFFGW AEYIVYHQLI DDLENLEVFT
     DILLYLDKAF LLLINDHILF MKTLLHSYMR DLRIKDKAAF ELINFKSFWK FFVKNCANMI
     EMDSSLQNQI AEVFELERHR KDHFTKMPNE TVSLYLEITG NKNTKFEEQN FPSVIQQNIK
     KVLHNPEDES NCRKLLLLCK ASHASEYNKF LSIFVKRGDF TVHELLKLIS LKLLSFEVIQ
     KTSHFDLLCE LVSQVSFNYG LNFENEKNAF VKRNFKQITL QLFSRPTFRD TLVRMLVEYG
     PNSNLSEKSA QVVSHILREE KNMSIIRDML IYGMNIENEA IDNTIDLYRY LNFTNTWLFQ
     ILTEFNVKNS NAEDLSDFFS SIVGAIGYNS LLPKIFSNIS DKKQVSTLLT VIERQFLARV
     LETEKSALVF LHIMMDTEIV LSRHLVNMSS LGMDSEVLEL FKNTIATFAK MPNDTLKKYD
     ENLNELLKIV IIHEKFILRH CFESIELRDH YMVDNFYTLF HNTGKDLKLK LLLYDLLTSL
     KSYILNETKD QALHSIKMPS VLNQLPKFAI SSFLDSEGTE DEFLDKDEPS LIRLGICDKQ
     AKTNSALKYF VFNKKSSQFD CIFKIEPFQY LVNYQEPLEG ELNNTSLSLG LFDARLEKTN
     PS