ID   SRB8_NEOFI              Reviewed;        1612 AA.
AC   A1D887;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 12;
DE   AltName: Full=Mediator complex subunit 12;
GN   Name=srb8; Synonyms=med12; ORFNames=NFIA_071020;
OS   Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164
OS   / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Fumigati.
OX   NCBI_TaxID=331117;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181
RC   / WB 181;
RX   PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA   Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA   Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA   Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA   Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA   Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA   White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA   Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT   "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT   fumigatus.";
RL   PLoS Genet. 4:E1000046-E1000046(2008).
CC   -!- FUNCTION: Component of the srb8-11 complex. The srb8-11 complex is a
CC       regulatory module of the Mediator complex which is itself involved in
CC       regulation of basal and activated RNA polymerase II-dependent
CC       transcription. The srb8-11 complex may be involved in the
CC       transcriptional repression of a subset of genes regulated by Mediator.
CC       It may inhibit the association of the Mediator complex with RNA
CC       polymerase II to form the holoenzyme complex (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of the srb8-11 complex, which itself associates with
CC       the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC       {ECO:0000305}.
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DR   EMBL; DS027690; EAW21931.1; -; Genomic_DNA.
DR   RefSeq; XP_001263828.1; XM_001263827.1.
DR   AlphaFoldDB; A1D887; -.
DR   STRING; 36630.CADNFIAP00005560; -.
DR   EnsemblFungi; EAW21931; EAW21931; NFIA_071020.
DR   GeneID; 4590474; -.
DR   KEGG; nfi:NFIA_071020; -.
DR   VEuPathDB; FungiDB:NFIA_071020; -.
DR   eggNOG; KOG4522; Eukaryota.
DR   HOGENOM; CLU_002034_1_0_1; -.
DR   OMA; DNKREAW; -.
DR   OrthoDB; 285540at2759; -.
DR   Proteomes; UP000006702; Unassembled WGS sequence.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR019035; Mediator_Med12.
DR   Pfam; PF09497; Med12; 1.
DR   SMART; SM01281; Med12; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1612
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   12"
FT                   /id="PRO_0000312974"
FT   REGION          18..114
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1514..1547
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        63..77
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        95..113
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1612 AA;  177653 MW;  59802224655CD774 CRC64;
     MIPHSSAGVQ SWGQPLHAVY SGTGRADLSQ PLGQPDRQPE QPSMPVPQLQ GRPPALIDLT
     TNDGDVLERE PPAKRLKIDV HAGSVAGDGS PASAGVGESK STPSATTSKP PSLSWRARPV
     WSFQALLSEV SGSAEINGES AAGVVQDVKP PPPPSFPGPP WKFAPADTIA SDSAGAQDGA
     PSKEVQTTPY HIETPSVAPV IRGEKVADFS PWMGNHPEDV LNEQTAKQGY YDRTQVSQNE
     SNTARPSLYA QLKHRSGLQI LSSVFAAALE KRQGHNMVTA PSTFKPPPRV TLTDNKREAW
     LRDLANPNVP LRKLSRTIPH GIRGRVLLDQ CLTKWVPVGR AVWLAKCVGA NEIRAFKRKG
     TSGALAIGLE AKWVRDWTAN VQQFLEGVIT SCGVADWKMK MTYAVSLTAR LFFEQLLDHD
     QYLGWFLTSL EAAPFNTLPV WLLMLGIYWS NILRYRKRGR RLAELLLDKL QVAIKSDSAT
     SLRPLTDRLS LHIRKLTLEH TSSMVLPQSW EKYKDLLSSC LDLNDNVHRA VFQNLAERNA
     RVQRPRKCEE TTQQPPQQRV IQLFDSIGSS NDITSVSAAS LGAIDDKAAL VLKLLEWAAT
     PFRYGVSRVY TGARLLRKWK IAGVDVDTSI ISFLGESQMR DQLNMDNIYH IVSELVRSQT
     FSVGKYLQWL MAKGVADFPQ NSDHQPLSGD LALLMQLPVS RLPEHVHNLR NTLLHRAGVE
     VSKESSTIAI LKASIAERLP RIFGSVATSA VSRDPLPSDL TWAVKSELGQ WIRRGVTEFG
     RDPRRAFQDL HSAPSAEHFA LTPGEFYTVR DILESFGDLS ILADVLKQAT VCNDGIVLAS
     AADTVNYHFR SFCVIGATTD LFKRLVESYA RLKRLGSTSL DLIFSLIDLG LRLPGELNTV
     ALLRQDLSRI ESKSSMAAPS PLSDHIPSSF NETDPLFLLK LDQLLSSASG IDESTLDTIF
     NLLIKQIESS GGHAKLSVNE TCRYLSYLRP FHPKRFDIMI VRWICGLLRS TTGGILSQVL
     PPLIGVGCVT IQAFVFLVRR LLKSENMMSN QRDLRIDLLQ LLVPPPAGQS RYFDMVTYRF
     HLSRKEFLFK HPEEVFDIIR DAIALIDSQS QEGNYRQVDL GHSAMVLLQI LLTKNPESAV
     KHCTEKLIGQ HPSAVTVLTR ALDSLLGLDT KAGERLFTSN GSFIFITIDT GPAAPDISVA
     EKVIELTNDF SLPFCRLKLQ LLFNAETKGD VRNEIVDVMF KAAVADSRSR RSNWVGLVRL
     MSHDAVRQIR ERAEKNFFAI PLFEESPDGC SSFAADNSSS LETAKLYLAI IEKLAYSIPD
     VGPQAVVPVL TEKMDLLLQR LITMQANYSG TTELSHGVDA EHMIRSRTQF ERALAFWFSA
     LLRMIVLHRT AFSVPSAAVR PSALPEQTRL LISIFCITLA RLPDSVLRLF PAADYFPHSM
     TAGDCRPCPG ILLQTHALDV AASLIDTFPD EARHQCARYL REKCPPFARV QNDSRFLYLL
     GPLGDSPSSN ITLPVSIPSP AASGSTPAPT PSGNPTGGFS HPQQPAFVSG VPTGLPDGLN
     CAASHLCLQY RGRAIGAYPV RPWELLEDAA PIAGTNDTAV SLGYFDARRV RV