ID   SRB8_NEUCR              Reviewed;        1789 AA.
AC   Q7SGG0; V5IPX3;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 12;
DE   AltName: Full=Mediator complex subunit 12;
GN   Name=srb8; Synonyms=med12; ORFNames=B20J13.130, NCU00962;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12655011; DOI=10.1093/nar/gkg293;
RA   Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA   Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT   "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT   genome sequence.";
RL   Nucleic Acids Res. 31:1944-1954(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Component of the srb8-11 complex. The srb8-11 complex is a
CC       regulatory module of the Mediator complex which is itself involved in
CC       regulation of basal and activated RNA polymerase II-dependent
CC       transcription. The srb8-11 complex may be involved in the
CC       transcriptional repression of a subset of genes regulated by Mediator.
CC       It may inhibit the association of the Mediator complex with RNA
CC       polymerase II to form the holoenzyme complex (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of the srb8-11 complex, which itself associates with
CC       the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC       {ECO:0000305}.
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DR   EMBL; BX842629; CAE76332.1; -; Genomic_DNA.
DR   EMBL; CM002236; ESA44213.1; -; Genomic_DNA.
DR   RefSeq; XP_011392868.1; XM_011394566.1.
DR   AlphaFoldDB; Q7SGG0; -.
DR   SMR; Q7SGG0; -.
DR   STRING; 5141.EFNCRP00000000568; -.
DR   PRIDE; Q7SGG0; -.
DR   EnsemblFungi; ESA44213; ESA44213; NCU00962.
DR   GeneID; 3881323; -.
DR   KEGG; ncr:NCU00962; -.
DR   VEuPathDB; FungiDB:NCU00962; -.
DR   HOGENOM; CLU_002034_0_0_1; -.
DR   InParanoid; Q7SGG0; -.
DR   Proteomes; UP000001805; Chromosome 1, Linkage Group I.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR019035; Mediator_Med12.
DR   Pfam; PF09497; Med12; 1.
DR   SMART; SM01281; Med12; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1789
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   12"
FT                   /id="PRO_0000312975"
FT   REGION          1..138
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          156..288
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1592..1656
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1707..1727
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..70
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        83..121
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        266..288
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1593..1615
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1631..1656
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1789 AA;  198740 MW;  B5E218FF401E3114 CRC64;
     MTSRPPLGVQ QRQPQQRKLS GPVLSQQRSL SQSQAQAQAQ AQQQQQQQQQ QQQQQQQQQQ
     QQQQQQYDAS SYLPPPPPVP TRNNNHNNFS NPNNTLNNAF PPELAQQDPT SIVSPADPSG
     SSPALSVGVG RYGTQRRGGS RLRLELSHND PFDAFSSPTV IESPGLVEPH TQNGSLTHPM
     MPLADGPDLG DMSPHHRVSI PPQHLDADVP IPFPQRRPKA VPDHSRREQP PPPPNPARKD
     TRPKPYTIEL PAAAPRYRIR GRSDGQGGQG RSSALATASS SSTTGSSATA NYGCADFFPW
     SGTAGNHPED QFNDTAIRVG YSEKQFVTPE TQSAKTVLFH GLKQRSGLNA LSIFFAGVLW
     HRRNAGQVTA PSTFKPPPRV TLTDTKKEVW LKDLANSAIP LRKLSRSIPH GVRNKVLLDQ
     CLNKNVPIDR AVWLAKCVGT NELRTFRRLG KGNNASVLEG ERKWLKDWTL VIENFIERVF
     FSFGEQNWKA KVNYAIQLAA HLYAEGLMDR EHYLDWTVSS LESSHHSQLP MWIIIAQIYW
     KDLLRLRKYG RRLATALISH YHVIHAHEDR DIYVPLLSKL SHLLGTLMVA TPENFVSPSV
     WVKYRDALKT CLPQGDEARH RSFAGINYRN EQLVASANRS QPAARIILVR NLDQVILEKP
     MPDEFPAQCW TVSKDKAALV RALLEWCTTP YRPGLAKVYV AHRILMHWST LRLDVTSAVL
     GFMEGEALEE MECKDALYHL VSELVRSGIF LVSQYIQWLI TRGGLTDPQL VMPDGPPFSR
     LLAELPSHIL GSSQKRSRDA LLRRAGFRIA DDAQDADMAI RHLRHALGLP AGVTDAVSMG
     RPFPIKKIAR RIEDASRARK AEIASWLRNT VFGGELEHSS LNAFGQHDLS TRLFNSIRTV
     FEAAQEFSVF ADVLRMLTKC SNPEVLASIA DTITRYTFVF AALSCLKNLF SILHSRLREV
     QEQGIGARPL LASLAYLASR VPGMDEVAMQ LKSDLAVSDR HNPVDACSPV SDNMVSRLSD
     HDGDLHEEVE KLLANGTSLD RNTMDRLFSA VIQRLQAYWS KGDEQQRVYG ILLKRLRMFN
     PQHFDELMTR WLLYVRGLDT RATILHLYPL LVSIGCLEMP AILATASESP NALGAGNSRH
     PQSAVATSQI VQTTFRTRYM QEVLQLFMAP VSSESLLTPE ERYRFGILQE QAKKESPREL
     LCLIQLALAE YTCARAQNDL EELPLDKEAN QDRLLRLLKL LVLKDPVGVA KALQIRGPDA
     QIDGWIDRMT TKLLVTAAGE GTRVTFEQVL NLTNEFTLPF CHVKLLLSLS SNDQQQNAAD
     SGERVPSNVE LFMNAMEKSM DAQRVSWIGM LPSLSPDLTH HLKNQAQARF LNLLPSARNP
     PSMTLDRSML QMRLQKAENF LAIMDTIIRG SGPMGFRQHQ LVPVMVERFT DILELLQTLT
     PFPSQAGGLG GWGGQGASAA AAGGDSHIDL KSDILNHWLP LFLNFLTLHA QTFDTSKPGN
     EVRGRALMVC AGLIRELDLI HGPDFDTRQL GGRIFDLACV LVDNLAEDAR LQCIQALKSP
     SDVKLRYIFS YQDNPHANLM LCHKERPTAM AIGTATGTGS PAPGTTPTHT PGVTPGPQNA
     VGAGSAVSGA GGSGNGGSYF SLPGHTQQAQ AQGWHAAQQQ QQQQQQQQSQ QQQQQQQQQQ
     YQLQQQQQQG GTGPIPSPAI SINTNLTHLN LGNNSNPPTP SPMNPTRPAM MMVANPWGGY
     SWVPTMGGPQ ERLTPFNFRQ WEMLSEPTSQ VGENDTAVNL MLFESRKVQ