SRB8_PICST
ID SRB8_PICST Reviewed; 1654 AA.
AC A3GF47;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 19-JAN-2010, sequence version 2.
DT 25-MAY-2022, entry version 70.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 12;
DE AltName: Full=Mediator complex subunit 12;
GN Name=SRB8; Synonyms=MED12; ORFNames=PICST_86078;
OS Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS Y-11545) (Yeast) (Pichia stipitis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX NCBI_TaxID=322104;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545;
RX PubMed=17334359; DOI=10.1038/nbt1290;
RA Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA Passoth V., Richardson P.M.;
RT "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT yeast Pichia stipitis.";
RL Nat. Biotechnol. 25:319-326(2007).
CC -!- FUNCTION: Component of the SRB8-11 complex. The SRB8-11 complex is a
CC regulatory module of the Mediator complex which is itself involved in
CC regulation of basal and activated RNA polymerase II-dependent
CC transcription. The SRB8-11 complex may be involved in the
CC transcriptional repression of a subset of genes regulated by Mediator.
CC It may inhibit the association of the Mediator complex with RNA
CC polymerase II to form the holoenzyme complex (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of the SRB8-11 complex, which itself associates with
CC the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC {ECO:0000305}.
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DR EMBL; AAVQ01000001; EAZ63285.2; -; Genomic_DNA.
DR RefSeq; XP_001387308.2; XM_001387271.1.
DR AlphaFoldDB; A3GF47; -.
DR STRING; 4924.XP_001387308.2; -.
DR PRIDE; A3GF47; -.
DR EnsemblFungi; EAZ63285; EAZ63285; PICST_86078.
DR GeneID; 4850896; -.
DR KEGG; pic:PICST_86078; -.
DR eggNOG; KOG4522; Eukaryota.
DR HOGENOM; CLU_003142_0_0_1; -.
DR InParanoid; A3GF47; -.
DR OMA; EKFEIHW; -.
DR OrthoDB; 285540at2759; -.
DR Proteomes; UP000002258; Chromosome 1.
DR GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR InterPro; IPR019035; Mediator_Med12.
DR Pfam; PF09497; Med12; 1.
DR SMART; SM01281; Med12; 1.
PE 3: Inferred from homology;
KW Activator; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..1654
FT /note="Mediator of RNA polymerase II transcription subunit
FT 12"
FT /id="PRO_0000312977"
FT REGION 52..72
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1481..1530
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 52..70
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1481..1499
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1515..1530
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1654 AA; 189211 MW; 10550E6CBCF9B173 CRC64;
MSKARSRNSL LSSSNRAQFG NSTQDELLKL KFSMEKPDIG LYPLNDLDNG VDLNGDNSRS
QHTAGSGVLS GNEVTYPDYK PWKDHTALPN DKKEQEHQKL NNAAYLNKGY FETPVVANEY
YSARNLIQAT VFSSTENCNE VLKELSQHLA NAYKTRNEII NKIKYESNNF KIPPRVTLTA
SKKESWLKDL ANPDLALSKI AEKIPHGIRN KILIDAVCNK SVPINRALWF TKCVLFGELV
ALRRKHQSRM SLNSPIPQSL DINTPEKFEI HWLQEWTQQV ADYIYKFSKE SSNFNTIERK
QYYMNKMTYL LTYIQALYVE FLLDKSFFLA LIIKFLKEGL PLDPLHVSEL LSSTRSETDD
LIQESWIEDL DLNYGQRLFA LTLIKIFWND ILKFDYICKE LSETLLLNYL FISKINAYSF
KQSHVQNHKA SIPEPLRQKI LDMIGDTITY LFKFNTNVFI IPNYWMLING VLFTILLNKN
VTKTEGELDE ISKQFDLIKY RNESLILNMR NVQPSITDRA TPNSAGRRGS SIWNQSFVSA
AESTATKIDI FDNEATFINR SSDDILKIIS QLDSLKLNDE LANFLKPVTS SSALTSTAIK
GCPKWRTNLK VVLYWCITRH RNSRESSEDI LIICNFLKRK VLQTLGPTRS SSQLKAEFES
EILDIIYNIA DTNSSKVVNY DLYVLINELY QLKVLTIASY LRKLIASGIF YVAPDAEDNI
LNDNSNSLVK THLSILQNLP VINNRQCDSI LKKWTSTGFN FKEKFEMGQE ILKRELIDRI
VNNTFDDQFE SHIVYVKDLN VGLKFLLVNW VTNELKSAIT ESPKLIHINP LIISNLFNFY
SICDNLTVFF KVLVKFILKN EGGMIIFYLE SLYLIARLII KHFKLVKFIA GNSYGSNSTA
YELFKLIIQN YKDCKTREFD YFKFDQVWNF IDTAVESNYS SDKNTDSRSS GKRSGIFNKE
QFDSPMKINT SENVIAKMED RYTSADFRND LDLLLESTFQ PMDSNEVSEV VATLKLEFEE
NEMKSHRNVV PKVLDLLKSN LTEESEGLAA KLLINSQYLI KSDDVNAFDK LVQDYILELV
KSDMEILLVA KFLKKLIVHE IIRINDLFAF FEPIAEDPAF RVKLKALMFD LVIGLSDEER
EYLSNSQILQ LEIMRQWYRE RSTSSFLVLI LKGIKTIEGS IFDCPLMEKY GSSIFRILNA
LIVANTKLLS DELISKISTE DSIRLLSTLN NENFTPINSL QDLERIASEV DEFNLPIFQL
LLRVLTIKEL SPLQENEIQE RLKVLLESFL ENLSFGFTPM NSYFGELFIY LPWEYVVSIL
GMLEDKFLCS TTFNFDQWDN DKSVLSLTNS VGNTNLLPVF NDYFKKFSSS SSNVVESSSS
FFQALSKFLS KLLLIVNSDN CLEDTFEDTS SAISIFLRIL IIHKLTLTRL IVTQDGEQFQ
FIKNLILLIN SKFIAEGNEK LRILLYDLLL LMKSSVTEEV SKQTENELSE GTSPGFGMTA
QSPPPVEDAS KITEPLSSAR PSSEAASFQT NPISTYDQVS SLFNLPEPTE TNPFKDYITE
DRVECALTLS EDELQSGGDI HGFNESNLVL ISSSNDSTFS GAFALITNPH QRPKGQPFKL
RSFEILEGTS TTSLNDGCIN LQLFDSYTTK ENPP