SRB8_SCHPO
ID SRB8_SCHPO Reviewed; 1134 AA.
AC Q9P6L8;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 2.
DT 25-MAY-2022, entry version 100.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 12;
DE AltName: Full=Mediator complex subunit 12;
DE AltName: Full=Suppressor of RNA polymerase B srb8;
GN Name=srb8; Synonyms=med12; ORFNames=SPAC688.08;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION, AND IDENTIFICATION IN THE MEDIATOR COMPLEX.
RX PubMed=12738880; DOI=10.1073/pnas.1030497100;
RA Samuelsen C.O., Baraznenok V., Khorosjutina O., Spaehr H., Kieselbach T.,
RA Holmberg S., Gustafsson C.M.;
RT "TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally
RT conserved through evolution.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:6422-6427(2003).
RN [3]
RP FUNCTION.
RX PubMed=14534314; DOI=10.1074/jbc.m306750200;
RA Spaehr H., Khorosjutina O., Baraznenok V., Linder T., Samuelsen C.O.,
RA Hermand D., Maekelae T.P., Holmberg S., Gustafsson C.M.;
RT "Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent
RT transcription in vitro.";
RL J. Biol. Chem. 278:51301-51306(2003).
CC -!- FUNCTION: Component of the srb8-11 complex. The srb8-11 complex is a
CC regulatory module of the Mediator complex which is itself involved in
CC regulation of basal and activated RNA polymerase II-dependent
CC transcription. The srb8-11 complex may be involved in the
CC transcriptional repression of a subset of genes regulated by Mediator.
CC It may inhibit the association of the Mediator complex with RNA
CC polymerase II to form the holoenzyme complex.
CC {ECO:0000269|PubMed:12738880, ECO:0000269|PubMed:14534314}.
CC -!- SUBUNIT: Component of the srb8-11 complex which consists of rb8,
CC srb9(TRAP240), srb10 and srb11. The srb8-11 complex associates with the
CC Mediator complex thereby blocking association with RNA polymerase II
CC and leading to reduced transcriptional activation by Mediator.
CC {ECO:0000269|PubMed:12738880}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC {ECO:0000305}.
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DR EMBL; CU329670; CAB90774.2; -; Genomic_DNA.
DR RefSeq; NP_594066.2; NM_001019490.2.
DR AlphaFoldDB; Q9P6L8; -.
DR BioGRID; 279887; 4.
DR STRING; 4896.SPAC688.08.1; -.
DR MaxQB; Q9P6L8; -.
DR PaxDb; Q9P6L8; -.
DR EnsemblFungi; SPAC688.08.1; SPAC688.08.1:pep; SPAC688.08.
DR GeneID; 2543467; -.
DR KEGG; spo:SPAC688.08; -.
DR PomBase; SPAC688.08; srb8.
DR VEuPathDB; FungiDB:SPAC688.08; -.
DR eggNOG; KOG4522; Eukaryota.
DR HOGENOM; CLU_278636_0_0_1; -.
DR OMA; WFIRCAG; -.
DR PRO; PR:Q9P6L8; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0016592; C:mediator complex; IPI:PomBase.
DR GO; GO:0003713; F:transcription coactivator activity; ISO:PomBase.
DR GO; GO:0022408; P:negative regulation of cell-cell adhesion; IMP:PomBase.
DR GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IC:PomBase.
DR InterPro; IPR019035; Mediator_Med12.
DR Pfam; PF09497; Med12; 1.
DR SMART; SM01281; Med12; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..1134
FT /note="Mediator of RNA polymerase II transcription subunit
FT 12"
FT /id="PRO_0000072182"
SQ SEQUENCE 1134 AA; 131246 MW; 1265D1BA5D5B5565 CRC64;
MSNRDSANPP GFNDAVPLAI CYIDSSTSSH DEYLTLLQSK VSAPDKNFRD ETVDGAPFLA
GDAISKGHRY SPPVRSDRAS AQGLLQQHLE KSSTLENLKR YSSTLLGRQN DAVIDEPFAP
FRPPPRVTLS ESRRDRWLQG LSDPMVPLSS LIKTIPHGLW GEDILRMLVK FRVPFTRAIW
FIRCAGVNEA RSFLRKVQTT DITEWVKNWT DVAAGFLISF ISSFLNADIY SFADDYTYLL
KLFGRLLAEE LVSPKHFLLR IVSFSGDSSL KSFSLHFFAL QFFSTSLIQY THICRKCVIT
ILQSYQQLIV DQPANLLKFS LLSKKVSHFL FTLAQKNIES FFFPTEWDKL KPTIILLWKD
FPNYSTLLSI MQERNSKAMY MYKPVTSSIR FLQIISCLSF PVGWRTLAKD LFKLLPVYTG
VPLLLHWCIN CRSIFSGDRN FIVSSIFDNA NFDRNLIVDL TLSFVLKLHP LEYNECVAAA
QLLDHLAACG YFFFSKYIAR LASLGYLRES MLNSSFMDDQ RKILVQLPIL RMSQQLKNKI
YYILSKGNYF VDWSICDEYV KRFKEDHFSF MFKKEENYAI ITLSLVKIAS TPMSKLYEDY
LVMLFAFHYS MFQVMTKLIA DNLVHFSFQS CACQTLFFIC SVVPKTESQK LLLNEMGKLF
QVELNFSYDS PDVNLLIEQF YEITSYESNY DDAFVEYKDA TVANRKDFIE FLFHNITVSS
KHTAVIFTSD LLMVLKIALN HPPYFDDLAT TTFSSLLKRD ECTILSFFKI LQFYGCKLLS
VDQIWAVVSD VYEAQDNNTT LKQFFNYLLD ESTWPEGYLE ERHWRSILCK EARKHDSGLK
LFKLGIKLCT RNEQIMKTIW SFIHVHDCNV ISEVIPDQRF LRTLTEHFMI DLRQLDIVTC
LKKALVTLDE FSAPLYATWL TTLDDDELSE LTDDVVQKKV LESLDNYKSG IWKLVLSGLP
NCKTVFEHLL LFSLEERLDL PAAFLQDLIG ASAYVMEQVP DSWFLEKLPC PLTQSLQSFS
HLSNHIEVLD STRQSRLTFL CHLILHMHGF VELTDQLATL ESLTIRKCIY RNQELLDLLL
FSIHLVKPNV ETNDEVCNTL KAWENIESRP YTIDFPEALQ QYSPRIVLYE PTFW
