SRB8_USTMA
ID SRB8_USTMA Reviewed; 1876 AA.
AC Q4P456; A0A0D1CU85;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 12;
DE AltName: Full=Mediator complex subunit 12;
GN Name=SRB8; Synonyms=MED12; ORFNames=UMAG_05107;
OS Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX NCBI_TaxID=237631;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=521 / FGSC 9021;
RX PubMed=17080091; DOI=10.1038/nature05248;
RA Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J.,
RA Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H.,
RA Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G.,
RA Snetselaar K., McCann M., Perez-Martin J., Feldbruegge M., Basse C.W.,
RA Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L.,
RA Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L.,
RA Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N.,
RA Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B.,
RA Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J.,
RA Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P.,
RA Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G.,
RA Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A.,
RA Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M.,
RA Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M.,
RA Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E.,
RA Birren B.W.;
RT "Insights from the genome of the biotrophic fungal plant pathogen Ustilago
RT maydis.";
RL Nature 444:97-101(2006).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=521 / FGSC 9021;
RA Gueldener U., Muensterkoetter M., Walter M.C., Mannhaupt G., Kahmann R.;
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the SRB8-11 complex. The SRB8-11 complex is a
CC regulatory module of the Mediator complex which is itself involved in
CC regulation of basal and activated RNA polymerase II-dependent
CC transcription. The SRB8-11 complex may be involved in the
CC transcriptional repression of a subset of genes regulated by Mediator.
CC It may inhibit the association of the Mediator complex with RNA
CC polymerase II to form the holoenzyme complex (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of the SRB8-11 complex, which itself associates with
CC the Mediator complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC {ECO:0000305}.
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DR EMBL; CM003143; KIS70033.1; -; Genomic_DNA.
DR RefSeq; XP_011388176.1; XM_011389874.1.
DR AlphaFoldDB; Q4P456; -.
DR STRING; 5270.UM05107P0; -.
DR EnsemblFungi; KIS70033; KIS70033; UMAG_05107.
DR GeneID; 23565086; -.
DR KEGG; uma:UMAG_05107; -.
DR VEuPathDB; FungiDB:UMAG_05107; -.
DR eggNOG; KOG4522; Eukaryota.
DR HOGENOM; CLU_237111_0_0_1; -.
DR InParanoid; Q4P456; -.
DR OMA; VSTEDWD; -.
DR OrthoDB; 285540at2759; -.
DR Proteomes; UP000000561; Chromosome 4.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR019035; Mediator_Med12.
DR InterPro; IPR021990; Mediator_Med12_LCEWAV.
DR Pfam; PF09497; Med12; 1.
DR Pfam; PF12145; Med12-LCEWAV; 1.
DR SMART; SM01281; Med12; 1.
PE 3: Inferred from homology;
KW Activator; Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..1876
FT /note="Mediator of RNA polymerase II transcription subunit
FT 12"
FT /id="PRO_0000312978"
FT REGION 1..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 291..314
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 715..737
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1373..1405
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1801..1876
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1825..1839
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1842..1856
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1876 AA; 204266 MW; 37F3EB60836D6977 CRC64;
MLRSGAASAS SGGGRGPSSS LSSVKRSKHD DTRLPEAYQQ ERPPWRPPLH PTAARRHVPD
LFPTKSGQPE DDMSQISVRK GFAPRTYVHN EYFCAHDAIH TRLLSPDALD TLSDWMDEVM
QRRRELDRAS IATNQYKPPS RVTLNDAKLA NYVKDLADPT VPLMRLSRSV PHGFRGEKLF
EMLWVGGALP STATAATTTV NYFHSRIAGP PSASSASASA SASASTSAPR KSVDIPRAVW
FIRALGAAEL SSLRNKSAAT IIPEITSNLC AWMAKQVAEL NLVHTAESNS TATASPAPVS
PSTHLPRTPS AAPSSLSRSV AAAHFARTPS NLNPASSPSL APAKEVCYVL QNDADEERWI
AKWSYSLSLA RHLHAQNLLD RSVLVRWIVD AFSASNLVQL PFLLELVQEV LLLVLRRRCF
VKPFLTALLV QIPNVDARLD PSSSGGLRTK LVLLFRIVCE NSPESLISPR LWFEHSASLT
QLLNELNAET PLPLSHQAFE FVEQTVRPRV DRLLLRPHSG NTTQNASLET QETLSSPLQH
HLDIQALDSF DMTTIDRVFL QNTSATHRYA KHDRDDTVWA TRIDTILTWA CTDRRSGVVR
QYLAATLIEK IRFGVPWEES SEETDQVSLP AFDLEHNTTK TRKINVEPML IKWLGDVETS
LNQKLDESST QTRVSLLATV DVGAIVVLLG ELARRGVFSY TKYLQRLIAR GMTAPSASSA
TEQHQNPDGS VQTPSSVSAS RDSLHLRLLR SLPLYDQPAS VYQQRRQAIY GDRTKETYED
AAQRRALRQL QSFLPFAFAQ EVQAEASRIA VSPSPSTPDP VHALSRLWSA SRFVRCRLFR
SELLPAVTAR VERLNGDQLS QISAVLVMAD DFEGLAQLLA TLLLRPLSDN LARAAFNMVI
EYSLVWRSMD IMATLNQLVR QQLNHSSSIR NDRQAVMAST TLLRLRTLIE NVPSRISASA
LDPGADHDAL QQHVQALRPN VDALLDRFAK LTRSSVESDG SIASSSTPVE GSDMLTPFRS
LFVQPDAMSD EMIERTIVDS VFARSDTSLV PSAVRLIDQL YLEASLEIDE RHARWLSNLV
LSIEQGTHSD ASMKALLSLL TRLIAHGTLN LQLAMDTFLL PYLLSSVRRL TDPATRNTEV
PMHIGSIVSS LGEIFASSLG ETVPATSYED MRAFGAQTKL LFAQVNLPSL LRAATCLISA
FDETSTVPAS EKAQIESFCN ALLQSQPMQI AFRQEPRLCI LAIKDTCKSM WGCNPSRVID
LAMACLDPTS LLLRDVGTID AASVKNRLDG WDTAVVATEL VEIFERLQLV ESSFQTRADS
KTKALASGIF DDLFVQLPDI GAQLVRDCQS SGLVSRFTDI GIKILSDKVK AASTDADKTP
RRMDNSHKGM TNFGPDDGAD APAQAKTAAD AAKHGPCVGE QQVESTLRSV VRMCEQQHSF
IFNATDADSC AQLLLHVVTS LEDALGSMAA ASHQDKVARL VTVLHGSMTF QLMHLVLRFG
CLWNGTMRAL ALRLVKALLE LTRQAGEKTE CGGSFALLLD SLSFVLDELP PTLLSTCLPE
LETQLQTIEL ATQERSDKLQ QLSFRLDNAT TANAWLVASS AGAALKKGWF TSGGLNPWEC
AEYLEAPPAA NPAAAGAAGA AGAAAGGNAR AGVVETGRSG VNSRAAVESP GAVTVNDAET
CNSLPGARTG WTGKLWLNTA IPLSMLGVRV TRDLVPNYAS PASPGGVSVG SNDASSQPWT
TGGGASAIGV GTCSAMDMDA KEALAALPMF VESERTYGER SAGEPAYSRD LRRGLLVEPN
LPCRTRDERG ETSQEPARAT MDPAGAHTSV TSSATHDPSH VIDLTWDADE EMPLSTHPHH
PAVATTSRKR RLSKRD
