ID   SRB8_YARLI              Reviewed;        1362 AA.
AC   Q6C229;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 2.
DT   25-MAY-2022, entry version 74.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 12;
DE   AltName: Full=Mediator complex subunit 12;
GN   Name=SRB8; Synonyms=MED12; OrderedLocusNames=YALI0F11341g;
OS   Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Dipodascaceae; Yarrowia.
OX   NCBI_TaxID=284591;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CLIB 122 / E 150;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the SRB8-11 complex. The SRB8-11 complex is a
CC       regulatory module of the Mediator complex which is itself involved in
CC       regulation of basal and activated RNA polymerase II-dependent
CC       transcription. The SRB8-11 complex may be involved in the
CC       transcriptional repression of a subset of genes regulated by Mediator.
CC       It may inhibit the association of the Mediator complex with RNA
CC       polymerase II to form the holoenzyme complex (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of the SRB8-11 complex, which itself associates with
CC       the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAG78090.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; CR382132; CAG78090.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_505283.1; XM_505283.1.
DR   AlphaFoldDB; Q6C229; -.
DR   SMR; Q6C229; -.
DR   GeneID; 2909018; -.
DR   KEGG; yli:YALI0F11341g; -.
DR   InParanoid; Q6C229; -.
DR   Proteomes; UP000001300; Chromosome F.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   InterPro; IPR019035; Mediator_Med12.
DR   Pfam; PF09497; Med12; 1.
DR   SMART; SM01281; Med12; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1362
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   12"
FT                   /id="PRO_0000312979"
FT   REGION          31..51
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1362 AA;  151979 MW;  99B4F7296CE7A278 CRC64;
     MNSSAPSPGP SSRLYRSINS RDDLSQQRYV LRPPDDIHPL VDPARIGDSV YPDFYPWKRS
     TDEEDARMVD NLQKGYAEPP SVPHESQSAK KSIASLLRIK SNMTALSCFI ITAMHIRVDT
     CRITTPGTFK PPPRVTLTDH KREAWLKDLA NPAVPLRKLS RTIPHGIRNK VLLDQCCAKR
     IAPSRAVWFA RCVGANELRG LKRKGHHQMG NMSEAIWLQE WTECVVDVLQ RSSVAFLRAE
     GEPQARQTAK QNFAYMWVVG LLSDDNTKDT KAGSGVAPGT GASTTSKLSS VEKLPVAILI
     IRTFWKPLLT FPNLAKKLAQ NLLAELSKLE ADAHSDKPFY QPTIKALSTL VNALFDAQPD
     AFVMPSKWVQ LGSTFEAIVS DSESTRVRNE ALVISDTPKT RRTRNKQAVI ISILDAARAP
     FDKTLFNQIV DTRADFGTII QTILAWAVSD SRVSVERVFI ASHLVSQLSE GVDGDAVPSH
     IVQFLLSVKS HLDVRIDDLY ALVTELCVLD IFQPQDYVRS LISSGVLYIS RLSEWANVQL
     DILGNLPLVA VPSSCQVQVR YMVRKVAKYA SYNENELLER TNNALRVLLP GVFDSQAEDV
     DMSSSSSSLS DSTNIPYSEF TQNTRIVVAE NLYTSLCAAV ERGYVPTTQE FAQYQLILEN
     LHAWRLMCAS IQLIVPKCTQ SHLLYFLASA TRYQWQAFAC IADMPALVKV FMHQYRGLRK
     VRVSRELWDL VQFSAQQLPE LKPELETLLK NSSSPQLSPI SEVTPQTDSE ATKDVNLPQA
     LNDLLLHSSD VRTSCKMLAN YKDDSQFSNQ LMTWLRDQEV SLQLLQTLVF LVVYECTCIE
     KVGDLFLQIH NLRDMSGGSR LLLSLIARDL TQEYSLSTTE AFSLQFQRKL FADQHPRIYL
     RLVAQYLFER EVFQDPQWLD DVDTFCKEVA VHHPALFSQL IVDPVIEPDN AVAASSLQQL
     LNHLLKLDLG TGTSLADDLQ QLITHVTPFN MFLAQTQLRL LFAHRGRISK DPVAMDTSPE
     LTINSTTPST DTTDEDVSVL FRVLSDPGLL KLPPFFGDLF SELPGELKGR ILARAETTFL
     TSPNFPRVGE DNIVSLLIEL ADSLADSVTE DVCKTQTFAL SSGLQRLVEA SVREPHPPGL
     AEGISMFLKI AMIHEKSLMA SEDAEVTMLR NHVVEGLQAL LDSPFVATTP DLKATLQDTW
     TALKSELSEV AEPANSAPSK AGPTRDLLLY STANDAFSEV TVRSFDLLEE SNPTMGVNDA
     ALNLALFNAT IEKRIPIAWN NGHKRQLVDG GRGGRKTARG TDSSLVPTIL EGIPRCMDEY
     SNHWELFFCF YLYTMRASEV HDLRANRASS FERRASSVEL LA