SRB8_YEAST
ID SRB8_YEAST Reviewed; 1427 AA.
AC P25648; D6VR82; P25647;
DT 01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1999, sequence version 2.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 12;
DE AltName: Full=Mediator complex subunit 12;
DE AltName: Full=Suppressor of RNA polymerase B 8;
GN Name=SRB8; Synonyms=GIG1, MED12, NUT6, SSN5; OrderedLocusNames=YCR081W;
GN ORFNames=YCR81W/YCR80W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7774808; DOI=10.1101/gad.9.8.897;
RA Hengartner C.J., Thompson C.M., Zhang J., Chao D.M., Liao S.-M.,
RA Koleske A.J., Okamura S., Young R.A.;
RT "Association of an activator with an RNA polymerase II holoenzyme.";
RL Genes Dev. 9:897-910(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=1574125; DOI=10.1038/357038a0;
RA Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M.,
RA Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G.,
RA Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A.,
RA Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M., Carcano C.,
RA Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M.,
RA Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C.,
RA Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F.,
RA Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C.,
RA Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E.,
RA Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P.,
RA Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J.,
RA Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P.,
RA Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M.,
RA Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P.,
RA Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G.,
RA Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E.,
RA Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F.,
RA Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L.,
RA Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J.,
RA Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M.,
RA Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A.,
RA Richterich P., Roberts A.B., Rodriguez F., Sanz E.,
RA Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J.,
RA Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I.,
RA Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M.,
RA Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M.,
RA Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D.,
RA Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K.,
RA Sgouros J.G.;
RT "The complete DNA sequence of yeast chromosome III.";
RL Nature 357:38-46(1992).
RN [3]
RP SEQUENCE REVISION.
RA Gromadka R.;
RL Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP IDENTIFICATION IN THE SRB8-11 COMPLEX, AND FUNCTION OF THE SRB8-11 COMPLEX.
RX PubMed=12200444; DOI=10.1074/jbc.m207195200;
RA Borggrefe T., Davis R., Erdjument-Bromage H., Tempst P., Kornberg R.D.;
RT "A complex of the Srb8, -9, -10, and -11 transcriptional regulatory
RT proteins from yeast.";
RL J. Biol. Chem. 277:44202-44207(2002).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP NOMENCLATURE.
RX PubMed=15175151; DOI=10.1016/j.molcel.2004.05.011;
RA Bourbon H.-M., Aguilera A., Ansari A.Z., Asturias F.J., Berk A.J.,
RA Bjoerklund S., Blackwell T.K., Borggrefe T., Carey M., Carlson M.,
RA Conaway J.W., Conaway R.C., Emmons S.W., Fondell J.D., Freedman L.P.,
RA Fukasawa T., Gustafsson C.M., Han M., He X., Herman P.K., Hinnebusch A.G.,
RA Holmberg S., Holstege F.C.P., Jaehning J.A., Kim Y.-J., Kuras L., Leutz A.,
RA Lis J.T., Meisterernest M., Naeaer A.M., Nasmyth K., Parvin J.D.,
RA Ptashne M., Reinberg D., Ronne H., Sadowski I., Sakurai H., Sipiczki M.,
RA Sternberg P.W., Stillman D.J., Strich R., Struhl K., Svejstrup J.Q.,
RA Tuck S., Winston F., Roeder R.G., Kornberg R.D.;
RT "A unified nomenclature for protein subunits of mediator complexes linking
RT transcriptional regulators to RNA polymerase II.";
RL Mol. Cell 14:553-557(2004).
RN [8]
RP TOPOLOGY OF THE MEDIATOR COMPLEX.
RX PubMed=15477388; DOI=10.1093/nar/gkh878;
RA Guglielmi B., van Berkum N.L., Klapholz B., Bijma T., Boube M.,
RA Boschiero C., Bourbon H.-M., Holstege F.C.P., Werner M.;
RT "A high resolution protein interaction map of the yeast Mediator complex.";
RL Nucleic Acids Res. 32:5379-5391(2004).
RN [9]
RP FUNCTION OF THE MEDIATOR COMPLEX.
RX PubMed=16076843; DOI=10.1074/jbc.m506067200;
RA Nair D., Kim Y., Myers L.C.;
RT "Mediator and TFIIH govern carboxyl-terminal domain-dependent transcription
RT in yeast extracts.";
RL J. Biol. Chem. 280:33739-33748(2005).
RN [10]
RP FUNCTION.
RX PubMed=16109375; DOI=10.1016/j.molcel.2005.06.033;
RA van de Peppel J., Kettelarij N., van Bakel H., Kockelkorn T.T.J.P.,
RA van Leenen D., Holstege F.C.P.;
RT "Mediator expression profiling epistasis reveals a signal transduction
RT pathway with antagonistic submodules and highly specific downstream
RT targets.";
RL Mol. Cell 19:511-522(2005).
RN [11]
RP FUNCTION OF THE SRB8-11 COMPLEX.
RX PubMed=15601835; DOI=10.1128/mcb.25.1.114-123.2005;
RA Larschan E., Winston F.;
RT "The Saccharomyces cerevisiae Srb8-Srb11 complex functions with the SAGA
RT complex during Gal4-activated transcription.";
RL Mol. Cell. Biol. 25:114-123(2005).
CC -!- FUNCTION: Component of the SRB8-11 complex. The SRB8-11 complex is a
CC regulatory module of the Mediator complex which is itself involved in
CC regulation of basal and activated RNA polymerase II-dependent
CC transcription. The SRB8-11 complex may be involved in the
CC transcriptional repression of a subset of genes regulated by Mediator.
CC It may inhibit the association of the Mediator complex with RNA
CC polymerase II to form the holoenzyme complex. The SRB8-11 complex
CC phosphorylates the C-terminal domain (CTD) of the largest subunit of
CC RNA polymerase II RPB1 at serines 2 and 5.
CC {ECO:0000269|PubMed:12200444, ECO:0000269|PubMed:15601835,
CC ECO:0000269|PubMed:16076843, ECO:0000269|PubMed:16109375}.
CC -!- SUBUNIT: Component of the SRB8-11 complex which consists of SRB8,
CC SSN2/SRB9, SSN3/SRB10 and SSN8/SRB11. The SRB8-11 complex associates
CC with the Mediator complex. The SSN3/SRB10 and SSN8/SRB11 kinase-cyclin
CC pair also associate with the RNA polymerase II holoenzyme.
CC {ECO:0000269|PubMed:12200444}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 1730 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC {ECO:0000305}.
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DR EMBL; X59720; CAA42268.1; -; Genomic_DNA.
DR EMBL; BK006937; DAA07551.1; -; Genomic_DNA.
DR PIR; S74293; S74293.
DR RefSeq; NP_010004.1; NM_001178787.1.
DR PDB; 7KPV; EM; 3.80 A; C=1-1427.
DR PDB; 7KPW; EM; 4.90 A; C=1-1427.
DR PDB; 7KPX; EM; 4.40 A; C=1-1427.
DR PDBsum; 7KPV; -.
DR PDBsum; 7KPW; -.
DR PDBsum; 7KPX; -.
DR AlphaFoldDB; P25648; -.
DR SMR; P25648; -.
DR BioGRID; 31054; 151.
DR ComplexPortal; CPX-1853; CKM complex.
DR DIP; DIP-1018N; -.
DR IntAct; P25648; 14.
DR MINT; P25648; -.
DR STRING; 4932.YCR081W; -.
DR iPTMnet; P25648; -.
DR MaxQB; P25648; -.
DR PaxDb; P25648; -.
DR PRIDE; P25648; -.
DR EnsemblFungi; YCR081W_mRNA; YCR081W; YCR081W.
DR GeneID; 850442; -.
DR KEGG; sce:YCR081W; -.
DR SGD; S000000677; SRB8.
DR VEuPathDB; FungiDB:YCR081W; -.
DR eggNOG; KOG4522; Eukaryota.
DR HOGENOM; CLU_256280_0_0_1; -.
DR InParanoid; P25648; -.
DR OMA; EFIVYHQ; -.
DR BioCyc; YEAST:G3O-29377-MON; -.
DR PRO; PR:P25648; -.
DR Proteomes; UP000002311; Chromosome III.
DR RNAct; P25648; protein.
DR GO; GO:1990508; C:CKM complex; IPI:ComplexPortal.
DR GO; GO:0016592; C:mediator complex; IDA:SGD.
DR GO; GO:0003713; F:transcription coactivator activity; IDA:SGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:0000411; P:positive regulation of transcription by galactose; IMP:SGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:0006468; P:protein phosphorylation; IDA:ComplexPortal.
DR InterPro; IPR019035; Mediator_Med12.
DR Pfam; PF09497; Med12; 1.
DR SMART; SM01281; Med12; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..1427
FT /note="Mediator of RNA polymerase II transcription subunit
FT 12"
FT /id="PRO_0000072183"
FT REGION 1042..1066
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1427 AA; 166861 MW; 776A2A9D82E331FE CRC64;
MNNGSGRYLL TPPDDLHPYV PSSKPQEQVY PDFKPWEHTA AEDQILANFV AKGFYHTPMV
NFESISARSS VHESLVTQSN ILSQQFDKII KIREDHINKI PSNSTTTLHG PGFQLPNRIT
LTDHRKETWL HELSSSHTSL VKIGKFIPHG LKRRQVIEQC YLKFIPLKRA IWLIKCCYFI
EWKSNHKKKR SNAAGADDAI SMHLLKDWTD TFVYILEKLI FDMTNHYNDS QQLRTWKRQI
SYFLKLLGNC YSLRLINKEI FHHWLVEFIN KMENFEFLPL SLHILMIFWN DICQIDTNAP
VAATITSSQK EPFFLVTKIT DMLLHKYYIV SSSKSMINDE NYIINDIKKN NKIKLNILKI
LSSLILKIFQ EQSLEVFIFP TSNWEIYKPL LFEIVSNADT NQNSDMKKKL ELISYRNESL
KNNSSIRNVI MSASNANDFQ LTIVTCKQFP KLSCIQLNCI DTQFTKLLDD NPTEFDWPTY
VDQNPLTMHK IIQLILWSIH PSRQFDHYES NQLVAKLLLL RINSTDEDLH EFQIEDAIWS
LVFQLAKNFS AQKRVVSYMM PSLYRLLNIL ITYGIIKVPT YIRKLISSGL LYLQDSNDKF
VHVQLLINLK ISPLMKSQYN MVLRNVMEYD VKFYEIFNFD QLVEITEQIK MRILSNDITN
LQLSKTPLSI KIMVAEWYLS HLCSGILSSV NRTVLLKIFK IFCIDLEVFH HFFKWIEFIV
YHQLLSDIES LEALMDILLC YQKLFSQFIN DHILFTKTFI FIYKKVLKEK DVPAYNVTSF
MPFWKFFMKN FPFVLKVDND LRIELQSVYN DEKLKTEKLK NDKSEVLKVY SMINNSNQAV
GQTWNFPEVF QVNIRFLLHN SEIIDTNTSK QFQKARNNVM LLIATNLKEY NKFMSIFLKR
KDFTNKNLIQ LISLKLLTFE VTQNVLGLEY IIRLLPINLE NNDGSYGLFL KYHKEQFIKS
NFEKILLTCY ELEKKYHGNE CEINYYEILL KILITYGSSP KLLATSTKII MLLLNDSVEN
SSNILEDILY YSTCPSETDL NDIPLGSGQP DNDTVVTNDD KSDDDDHTVD EIDHVEYYVM
MDFANLWVFQ AFTCFCIKKI MENNEPAMAM EDLKNFIFQI IEITNSNDLC SQIFDQLKDM
QTIEMITQIV EKDFCTSCLQ NNNQKIDDNY IVVVIEIITS LSMRFQRETS GMIVISMENY
HLLIKIIRQL SELNEGNLSK REIQIDAVLK IFSFHQDSIF QRIIADLSAD KPTSPFIDSI
CKLFDKISFN LRLKLFLYEI LSSLKSFAIY SSTIDAPAFH TSGKVELPKK LLNLPPFQVS
SFVKETKLHS GDYGEEEDAD QEESFSLNLG IGIVEIAHEN EQKWLIYDKK DHKYVCTFSM
EPYHFISNYN TKYTDDMATG SNDTTAFNDS CVNLSLFDAR FERKNPH
