SRCA_MOUSE
ID SRCA_MOUSE Reviewed; 472 AA.
AC Q7TQ48; A6H5Y7; Q8BS43;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 07-OCT-2020, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Sarcalumenin {ECO:0000250|UniProtKB:P13666};
DE Flags: Precursor;
GN Name=Srl {ECO:0000312|MGI:MGI:2146620};
GN Synonyms=Sar {ECO:0000312|MGI:MGI:2146620};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC TISSUE=Skeletal muscle;
RA Yoshida M., Komazaki S., Nishi M., Saito M., Yanagisawa T., Takeshima H.;
RT "Generation and characterization of sarcalumenin-deficient mice.";
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Bone;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-304 (ISOFORM 2), AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Heart, Kidney, and Lung;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Sarcoplasmic reticulum lumen
CC {ECO:0000250|UniProtKB:P13666}. Sarcoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:P13666}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P13666}. Note=May associate with the
CC sarcoplasmic reticulum membrane, via a calcium-dependent mechanism.
CC {ECO:0000250|UniProtKB:P13666}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7TQ48-2; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7TQ48-1; Sequence=VSP_060764;
CC -!- PTM: N-glycosylated. {ECO:0000250|UniProtKB:P13666}.
CC -!- SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase
CC superfamily. Dynamin/Fzo/YdjA family. {ECO:0000255|PROSITE-
CC ProRule:PRU01055}.
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DR EMBL; AB113383; BAC78629.1; -; mRNA.
DR EMBL; AK036394; BAC29409.1; -; mRNA.
DR EMBL; BC145687; AAI45688.1; -; mRNA.
DR CCDS; CCDS27918.1; -. [Q7TQ48-1]
DR CCDS; CCDS84202.1; -. [Q7TQ48-2]
DR RefSeq; NP_001334091.1; NM_001347162.1. [Q7TQ48-2]
DR RefSeq; NP_780556.2; NM_175347.4. [Q7TQ48-1]
DR AlphaFoldDB; Q7TQ48; -.
DR SMR; Q7TQ48; -.
DR BioGRID; 223048; 6.
DR IntAct; Q7TQ48; 1.
DR STRING; 10090.ENSMUSP00000023161; -.
DR GlyGen; Q7TQ48; 2 sites.
DR iPTMnet; Q7TQ48; -.
DR PhosphoSitePlus; Q7TQ48; -.
DR CPTAC; non-CPTAC-3877; -.
DR MaxQB; Q7TQ48; -.
DR PaxDb; Q7TQ48; -.
DR PeptideAtlas; Q7TQ48; -.
DR PRIDE; Q7TQ48; -.
DR ProteomicsDB; 257361; -. [Q7TQ48-2]
DR ProteomicsDB; 257362; -. [Q7TQ48-2]
DR Antibodypedia; 55440; 44 antibodies from 13 providers.
DR DNASU; 106393; -.
DR Ensembl; ENSMUST00000023161; ENSMUSP00000023161; ENSMUSG00000022519. [Q7TQ48-1]
DR Ensembl; ENSMUST00000090500; ENSMUSP00000087986; ENSMUSG00000022519. [Q7TQ48-2]
DR GeneID; 106393; -.
DR KEGG; mmu:106393; -.
DR UCSC; uc007xzt.1; mouse. [Q7TQ48-2]
DR UCSC; uc007xzu.1; mouse. [Q7TQ48-2]
DR CTD; 6345; -.
DR MGI; MGI:2146620; Srl.
DR VEuPathDB; HostDB:ENSMUSG00000022519; -.
DR eggNOG; KOG1954; Eukaryota.
DR GeneTree; ENSGT00940000157834; -.
DR HOGENOM; CLU_017595_4_1_1; -.
DR InParanoid; Q7TQ48; -.
DR OMA; GIIPENR; -.
DR OrthoDB; 377342at2759; -.
DR PhylomeDB; Q7TQ48; -.
DR TreeFam; TF324008; -.
DR BioGRID-ORCS; 106393; 2 hits in 58 CRISPR screens.
DR ChiTaRS; Srl; mouse.
DR PRO; PR:Q7TQ48; -.
DR Proteomes; UP000000589; Chromosome 16.
DR RNAct; Q7TQ48; protein.
DR Bgee; ENSMUSG00000022519; Expressed in interventricular septum and 171 other tissues.
DR Genevisible; Q7TQ48; MM.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0033018; C:sarcoplasmic reticulum lumen; IEA:UniProtKB-SubCell.
DR GO; GO:0033017; C:sarcoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:InterPro.
DR GO; GO:0006897; P:endocytosis; IBA:GO_Central.
DR GO; GO:0016197; P:endosomal transport; IBA:GO_Central.
DR GO; GO:0014873; P:response to muscle activity involved in regulation of muscle adaptation; IMP:MGI.
DR GO; GO:0002115; P:store-operated calcium entry; IMP:MGI.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR045063; Dynamin_N.
DR InterPro; IPR031692; EHD_N.
DR InterPro; IPR030381; G_DYNAMIN_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF00350; Dynamin_N; 1.
DR Pfam; PF16880; EHD_N; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51718; G_DYNAMIN_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Glycoprotein; Membrane; Phosphoprotein;
KW Reference proteome; Sarcoplasmic reticulum; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..472
FT /note="Sarcalumenin"
FT /id="PRO_0000045427"
FT DOMAIN 89..330
FT /note="Dynamin-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 99..106
FT /note="G1 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 127..128
FT /note="G2 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 189..192
FT /note="G3 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 254..257
FT /note="G4 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 277
FT /note="G5 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT CARBOHYD 280
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 388
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 20
FT /note="A -> AELQVSASGGTEDVGNLLENHFSAGDASLEEKERALYADAAPGDKNL
FT LLHYPDGREAESLEQTPAGAPSTANGQGSETEASLSNTSAAESAPPGDVEGPGEEEEGP
FT HAAGTLPPGGIEGPEEERPQFSSGKEPGQEEAGVGVPTEGAASMEAEVQEGSSGVPEEL
FT QGLLGDGPMNEAAGTAESVVTQSSPPIEGKEAEGEGNTGSDHQSTELDGTQDAIPAGDE
FT SEAREGAEDKPQDYSLQSEDTATPGPDHQPTDLHAASDTASSGVEAEEGAEESKAEVDT
FT ESGEKVEDQGEPRPSLDAGSAIEANKQSEYTQARETENDSPEGQVSGMSEEGLAEASSE
FT EEGGEEDGSENGEVPFKEESGEDSGDGASSEEEGSTPGEAGEPQKTPGATGHEEEGPQL
FT DVEDLNTGFEGSKTQDIEAEASEERQQERGNPVIAQWE (in isoform 2)"
FT /id="VSP_060764"
FT MOD_RES Q7TQ48-1:304
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CARBOHYD Q7TQ48-1:103
FT /note="N-linked (GlcNAc) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 472 AA; 54287 MW; 054AF34A741D09C7 CRC64;
MKALLLLCCF LASLLLSGQA EVEDASEEAP LRDRSHIDKT LMLNEDKPAD DYSAVLQRLR
KIYHTSIKPL EQSYKYNELR QHEITDGEIT SKPMVLFLGP WSVGKSTMIN YLLGLEDTRY
QLYTGAEPTT SEFTVLMHGP KLKTIEGIVM AADSARSFSP LEKFGQNFLE KLIGIEVPHK
LLERVTFVDT PGIIENRKQQ ERGYPFNDVC QWFIDRADLI FVVFDPTKLD VGLELEMLFR
QLKGRESQIR IILNKADNLA TQMLMRVYGA LFWSLAPLIN VTEPPRVYVS SFWPQDYKPD
THRELFLKEE ISLLEDLNQV IENRLENKIA FIRQHAIRVR IHALLVDRYL QTYKDKMTFF
SDGELVFKDI VEDPDKFYIF KTILAKTNVS KFDLPNREAY KDFFGINPIS NFKLLSQQCS
YMGGCFLEKI ERAITQELPS LLGSIGLGKN PGAPNCDKTG CGETPKNRYK KH
