SRD2_ARATH
ID SRD2_ARATH Reviewed; 375 AA.
AC Q8L627; F4HY74; Q9S7F0;
DT 01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=snRNA-activating protein complex subunit;
DE Short=SNAPc subunit;
DE AltName: Full=Protein SHOOT REDIFFERENTIATION DEFECTIVE 2;
GN Name=SRD2; OrderedLocusNames=At1g28560; ORFNames=F1K23.20, F3M18.1;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, AND MUTAGENESIS OF GLY-163.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=12232244; DOI=10.1104/pp.105.3.815;
RA Yasutani I., Ozawa S., Nishida T., Sugiyama M., Komamine A.;
RT "Isolation of temperature-sensitive mutants of Arabidopsis thaliana that
RT are defective in the redifferentiation of shoots.";
RL Plant Physiol. 105:815-822(1994).
RN [5]
RP FUNCTION, AND MUTAGENESIS OF GLY-163.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=9389671; DOI=10.1242/dev.125.1.135;
RA Ozawa S., Yasutani I., Fukuda H., Komamine A., Sugiyama M.;
RT "Organogenic responses in tissue culture of srd mutants of Arabidopsis
RT thaliana.";
RL Development 125:135-142(1998).
RN [6]
RP FUNCTION, AND MUTAGENESIS OF GLY-163.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=12881506; DOI=10.1093/pcp/pcg082;
RA Huang H.-J., Lin Y.-M., Huang D.-D., Takahashi T., Sugiyama M.;
RT "Protein tyrosine phosphorylation during phytohormone-stimulated cell
RT proliferation in Arabidopsis Hypocotyls.";
RL Plant Cell Physiol. 44:770-775(2003).
RN [7]
RP FUNCTION, MUTAGENESIS OF GLY-163, SUBCELLULAR LOCATION, AND INDUCTION BY
RP AUXIN AND CYTOKININ.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=16098103; DOI=10.1111/j.1365-313x.2005.02469.x;
RA Ohtani M., Sugiyama M.;
RT "Involvement of SRD2-mediated activation of snRNA transcription in the
RT control of cell proliferation competence in Arabidopsis.";
RL Plant J. 43:479-490(2005).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-163, TISSUE SPECIFICITY,
RP AND DEVELOPMENTAL STAGE.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=18064403; DOI=10.1007/s11103-007-9271-7;
RA Ohtani M., Demura T., Sugiyama M.;
RT "Differential requirement for the function of SRD2, an snRNA transcription
RT activator, in various stages of plant development.";
RL Plant Mol. Biol. 66:303-314(2008).
RN [9]
RP FUNCTION, MUTAGENESIS OF GLY-163, AND DEVELOPMENTAL STAGE.
RX PubMed=20965997; DOI=10.1093/pcp/pcq159;
RA Ohtani M., Demura T., Sugiyama M.;
RT "Particular significance of SRD2-dependent snRNA accumulation in polarized
RT pattern generation during lateral root development of Arabidopsis.";
RL Plant Cell Physiol. 51:2002-2012(2010).
CC -!- FUNCTION: Transcription activator of small-nuclear RNA genes (snRNA),
CC which have essential roles in pre-mRNA splicing and rRNA processing.
CC Essential protein involved in the establishment of apical meristems and
CC organ primordia, embryogenesis, cell differentiation and cell
CC proliferation, probably by modulating the establishment of auxin
CC gradients. Participates in the control of competence in cell
CC proliferation; required for the reinitiation of the progression of the
CC cell cycle and subsequent cell proliferation during cell
CC redifferentiation leading to callus formation, especially during the
CC first day of dedifferentiation (e.g. callus, root and shoot formation
CC from root and hypocotyl explants). {ECO:0000269|PubMed:12232244,
CC ECO:0000269|PubMed:12881506, ECO:0000269|PubMed:16098103,
CC ECO:0000269|PubMed:18064403, ECO:0000269|PubMed:20965997,
CC ECO:0000269|PubMed:9389671}.
CC -!- SUBUNIT: Part of the SNAPc complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16098103}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8L627-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8L627-2; Sequence=VSP_046407;
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, apical meristems,
CC developing leaves, roots, stipules and flowers.
CC {ECO:0000269|PubMed:18064403}.
CC -!- DEVELOPMENTAL STAGE: In young seedlings, accumulates in shoot and root
CC apical meristems (SAM and RAM, respectively), leaf primordia, and root
CC stele tissues. In roots, high levels throughout young root primordia
CC that later decrease at the arrest of cell division, and finally
CC increase again when the apical meristem become activated. In young
CC developing leaves, present in guard cells, vascular tissues, trichomes,
CC and trichome support cells to be later expressed uniformly in the
CC lamina. Gradually restricted to the leaf margin and fades out in fully
CC expanded leaves. In roots, detected in apices, stele tissues, and
CC lateral root primordia. In flowers, first observed over the floral bud,
CC and later restricted to vascular tissues in developing floral organs,
CC except for the pistil, where the expression is ubiquitous. In mature
CC flowers, disappears progressively from the pistil, but accumulates in
CC mature pollen and the filaments. Also present in pedicels. In
CC pollinated pistils, highly expressed in ovaries that contain developing
CC embryos. Present throughout embryogenesis, particularly in the lower
CC half of the embryo at the heart-shaped stage and in the radicle RAM
CC region at the cotyledonary stage. {ECO:0000269|PubMed:18064403,
CC ECO:0000269|PubMed:20965997}.
CC -!- INDUCTION: Transiently induced by auxin (2,4-D), with a synergistic
CC action of auxin and cytokinin on transcript levels.
CC {ECO:0000269|PubMed:16098103}.
CC -!- DISRUPTION PHENOTYPE: Lethal. {ECO:0000269|PubMed:18064403}.
CC -!- SIMILARITY: Belongs to the SNAPC3/SRD2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF16748.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAF24550.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007508; AAF24550.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC010155; AAF16748.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30992.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30993.1; -; Genomic_DNA.
DR EMBL; AY099624; AAM20475.1; -; mRNA.
DR EMBL; BT003404; AAO30067.1; -; mRNA.
DR PIR; D86411; D86411.
DR RefSeq; NP_001117373.2; NM_001123901.2. [Q8L627-2]
DR RefSeq; NP_174178.2; NM_102624.4. [Q8L627-1]
DR AlphaFoldDB; Q8L627; -.
DR STRING; 3702.AT1G28560.2; -.
DR PaxDb; Q8L627; -.
DR PRIDE; Q8L627; -.
DR ProteomicsDB; 226710; -. [Q8L627-1]
DR EnsemblPlants; AT1G28560.1; AT1G28560.1; AT1G28560. [Q8L627-1]
DR EnsemblPlants; AT1G28560.2; AT1G28560.2; AT1G28560. [Q8L627-2]
DR GeneID; 839756; -.
DR Gramene; AT1G28560.1; AT1G28560.1; AT1G28560. [Q8L627-1]
DR Gramene; AT1G28560.2; AT1G28560.2; AT1G28560. [Q8L627-2]
DR KEGG; ath:AT1G28560; -.
DR Araport; AT1G28560; -.
DR TAIR; locus:2018683; AT1G28560.
DR eggNOG; KOG2664; Eukaryota.
DR HOGENOM; CLU_041861_2_0_1; -.
DR InParanoid; Q8L627; -.
DR PhylomeDB; Q8L627; -.
DR PRO; PR:Q8L627; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8L627; baseline and differential.
DR Genevisible; Q8L627; AT.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0019185; C:snRNA-activating protein complex; IBA:GO_Central.
DR GO; GO:0003681; F:bent DNA binding; IBA:GO_Central.
DR GO; GO:0001006; F:RNA polymerase III type 3 promoter sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009926; P:auxin polar transport; IMP:UniProtKB.
DR GO; GO:0009734; P:auxin-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0060184; P:cell cycle switching; IMP:UniProtKB.
DR GO; GO:0043697; P:cell dedifferentiation; IMP:UniProtKB.
DR GO; GO:0071365; P:cellular response to auxin stimulus; IEP:UniProtKB.
DR GO; GO:0071368; P:cellular response to cytokinin stimulus; IEP:UniProtKB.
DR GO; GO:0010311; P:lateral root formation; IMP:UniProtKB.
DR GO; GO:0009942; P:longitudinal axis specification; IMP:UniProtKB.
DR GO; GO:0010014; P:meristem initiation; IMP:UniProtKB.
DR GO; GO:1905392; P:plant organ morphogenesis; IMP:TAIR.
DR GO; GO:0009945; P:radial axis specification; IMP:UniProtKB.
DR GO; GO:0009301; P:snRNA transcription; IMP:UniProtKB.
DR GO; GO:0042795; P:snRNA transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0042796; P:snRNA transcription by RNA polymerase III; IBA:GO_Central.
DR InterPro; IPR022042; snRNA-activating_su3.
DR PANTHER; PTHR13421; PTHR13421; 1.
DR Pfam; PF12251; SNAPC3; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Auxin signaling pathway; Cell cycle;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..375
FT /note="snRNA-activating protein complex subunit"
FT /id="PRO_0000422095"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 26
FT /note="H -> HRASSQYISKVF (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_046407"
FT MUTAGEN 163
FT /note="G->R: In srd2-1; temperature sensitive plants with
FT severe suppression of seedling growth and impaired shoot
FT redifferentiation in root explants at 27 degrees Celsius
FT but not at 22 degrees Celsius, however, reduced lateral
FT root formation at any temperature. Loss of reinitiation of
FT the progression of the cell cycle at the restrictive
FT temperature (e.g. 27 degrees Celsius) accompanied with a
FT global reduction in protein tyrosine phosphorylation and
FT impaired small-nuclear RNA transcription. Reduced trichome
FT branching, disordered vascular network and abnormal guard
FT cell shapes are also observed. Altered organization of
FT cells in root primordia, with abnormal apical/basal and
FT radial axes, and persistance of primordial cell division
FT resulting in the formation of abnormal hemispherical
FT laterals."
FT /evidence="ECO:0000269|PubMed:12232244,
FT ECO:0000269|PubMed:12881506, ECO:0000269|PubMed:16098103,
FT ECO:0000269|PubMed:18064403, ECO:0000269|PubMed:20965997,
FT ECO:0000269|PubMed:9389671"
SQ SEQUENCE 375 AA; 43527 MW; 10042F5371F35E52 CRC64;
MAMKEAFPED YLSQEELEPS LNVSHHENPL AGRAKRKRTV KNTEVKKRTL KNTEVMKMTE
KKTEEAYLVK VEQLAKLKQK QEEDKAAVTL HCFSKTSETG KDVVAPPEGF EQMQSLRFID
NNYTKLKPSD IQGQVDPLFP EVILCVEIYN SRKVKTQEFL VLGRQMLTEL KDNIHCATDQ
VMQKAGKYDP SGYFLIEDVF HNDLRNPSAK DYSYPILDWL WNSKDEALKK WECVLTGELQ
KKQKLVLGEA KSVDLPRYRT ADMQSTHFCD IRFRVGASYV YCHQGDCKHT IVIRDMRMSH
PEDVQNRAAY PIMFWPKRRI QKCGVCKIKR ASKVAVDDKW ASENSSYFCD VCFELLHSEE
GPLNCDFPVF DYVHE
