SRF3_CAEEL
ID SRF3_CAEEL Reviewed; 368 AA.
AC Q93890; O02348;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 5.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=UDP-galactose/UDP-N-acetylglucosamine transporter srf-3;
DE AltName: Full=Surface antigenicity abnormal 3;
GN Name=srf-3; ORFNames=M02B1.1;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 41-368, FUNCTION, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, MUTAGENESIS OF GLY-285, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=15123614; DOI=10.1074/jbc.m402429200;
RA Hoeflich J., Berninsone P., Goebel C., Gravato-Nobre M.J., Libby B.J.,
RA Darby C., Politz S.M., Hodgkin J., Hirschberg C.B., Baumeister R.;
RT "Loss of srf-3-encoded nucleotide sugar transporter activity in
RT Caenorhabditis elegans alters surface antigenicity and prevents bacterial
RT adherence.";
RL J. Biol. Chem. 279:30440-30448(2004).
RN [3]
RP FUNCTION.
RX PubMed=15452127; DOI=10.1074/jbc.m409557200;
RA Cipollo J.F., Awad A.M., Costello C.E., Hirschberg C.B.;
RT "srf-3, a mutant of Caenorhabditis elegans, resistant to bacterial
RT infection and to biofilm binding, is deficient in glycoconjugates.";
RL J. Biol. Chem. 279:52893-52903(2004).
CC -!- FUNCTION: Acts as a transporter of both UDP-galactose and UDP-N-
CC acetylglucosamine into the Golgi lumen. {ECO:0000269|PubMed:15123614,
CC ECO:0000269|PubMed:15452127}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC {ECO:0000269|PubMed:15123614}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15123614}.
CC -!- TISSUE SPECIFICITY: Expressed exclusively in pharyngeal cells g1 and
CC g2, lateral seam cells, spermatheca and vas deferens.
CC {ECO:0000269|PubMed:15123614}.
CC -!- DEVELOPMENTAL STAGE: In seam cells, expression is first detected in
CC late embryos, persists to the fourth larval stage and disappears in
CC adults. In glandular cells g1 and g2, expression begins in the first
CC larval stage and remains visible until adulthood.
CC {ECO:0000269|PubMed:15123614}.
CC -!- DISRUPTION PHENOTYPE: Worms display altered surface antigenicity which
CC prevents bacterial adherence. This is due to a deficiency in both
CC O- and N-linked glycans. {ECO:0000269|PubMed:15123614}.
CC -!- SIMILARITY: Belongs to the nucleotide-sugar transporter family. SLC35A
CC subfamily. {ECO:0000305}.
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DR EMBL; Z81102; CAB03205.4; -; Genomic_DNA.
DR EMBL; Z82288; CAB03205.4; JOINED; Genomic_DNA.
DR PIR; T23676; T23676.
DR RefSeq; NP_001255676.1; NM_001268747.1.
DR AlphaFoldDB; Q93890; -.
DR SMR; Q93890; -.
DR STRING; 6239.M02B1.1a; -.
DR TCDB; 2.A.7.12.4; the drug/metabolite transporter (dmt) superfamily.
DR EPD; Q93890; -.
DR PaxDb; Q93890; -.
DR PeptideAtlas; Q93890; -.
DR EnsemblMetazoa; M02B1.1a.1; M02B1.1a.1; WBGene00005153.
DR GeneID; 187393; -.
DR KEGG; cel:CELE_M02B1.1; -.
DR UCSC; M02B1.1; c. elegans.
DR CTD; 187393; -.
DR WormBase; M02B1.1a; CE43555; WBGene00005153; srf-3.
DR eggNOG; KOG2234; Eukaryota.
DR GeneTree; ENSGT00950000182827; -.
DR InParanoid; Q93890; -.
DR OMA; SIWMRNV; -.
DR OrthoDB; 703674at2759; -.
DR PhylomeDB; Q93890; -.
DR Reactome; R-CEL-4085001; Sialic acid metabolism.
DR Reactome; R-CEL-727802; Transport of nucleotide sugars.
DR PRO; PR:Q93890; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00005153; Expressed in adult organism and 3 other tissues.
DR ExpressionAtlas; Q93890; baseline and differential.
DR GO; GO:0005794; C:Golgi apparatus; IC:WormBase.
DR GO; GO:0030173; C:integral component of Golgi membrane; IDA:UniProtKB.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:WormBase.
DR GO; GO:0005459; F:UDP-galactose transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0005462; F:UDP-N-acetylglucosamine transmembrane transporter activity; IDA:UniProtKB.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0072334; P:UDP-galactose transmembrane transport; IDA:UniProtKB.
DR GO; GO:1990569; P:UDP-N-acetylglucosamine transmembrane transport; IDA:UniProtKB.
DR InterPro; IPR007271; Nuc_sug_transpt.
DR PANTHER; PTHR10231; PTHR10231; 1.
DR Pfam; PF04142; Nuc_sug_transp; 1.
DR PIRSF; PIRSF005799; UDP-gal_transpt; 1.
PE 1: Evidence at protein level;
KW Golgi apparatus; Membrane; Reference proteome; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..368
FT /note="UDP-galactose/UDP-N-acetylglucosamine transporter
FT srf-3"
FT /id="PRO_0000213363"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 235..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 317..337
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 285
FT /note="G->E: In e2680; resistant to infection by
FT M.nematophilum and prevents biofilm formation by
FT Y.pseudotuberculosis."
FT /evidence="ECO:0000269|PubMed:15123614"
SQ SEQUENCE 368 AA; 40733 MW; 26A1254C21DC7394 CRC64;
MMGRNDDAFH PLLHSTPESS VKIPVPLVSV GRESSQSKGN MKTAILIWLT LQNSIHTLLI
RYSRAREVDA MFVSTVAVWL TEVIKCFICL FLVAQEETPR RFIHALRTQI LEQPYDTLKV
CIPAMIYIVQ NNLFYVAASH LDAATFMITS QLKIFTAAIF TVIILRRSLN RTQWFALAVL
FVGVSLVQLQ GTKAKESSGE SPFVGFVAVV VACCLSGFAG IYFEKILKGS APVSLWMRNV
QMAVFSIPAS FSAIYMQDSK TVNEYGLLYG FDSIVWLTVL WYGVGGLSVA VCIKYADNIA
KNFATSVAII LSTIGSIFLF DFIPSFTFLL GASLVIFSIF LYSSHQSMVA ALGRLRGEIP
STKEAFCL
