SRFA_DICDI
ID SRFA_DICDI Reviewed; 418 AA.
AC Q54TY7; O76853;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Serum response factor homolog A;
DE AltName: Full=DsSRF;
GN Name=srfA; ORFNames=DDB_G0281387;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=AX4;
RX PubMed=9729488; DOI=10.1242/dev.125.19.3801;
RA Escalante R., Sastre L.;
RT "A serum response factor homolog is required for spore differentiation in
RT Dictyostelium.";
RL Development 125:3801-3808(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP EXPRESSION, AND DEVELOPMENTAL STAGE.
RX PubMed=11437439; DOI=10.1006/dbio.2001.0303;
RA Escalante R., Vicente J.J., Moreno N., Sastre L.;
RT "The MADS-box gene srfA is expressed in a complex pattern under the control
RT of alternative promoters and is essential for different aspects of
RT Dictyostelium development.";
RL Dev. Biol. 235:314-329(2001).
RN [4]
RP REGULATION BY PROTEIN KINASE A.
RC STRAIN=AX4;
RX PubMed=12204259; DOI=10.1016/s0925-4773(02)00203-4;
RA Escalante R., Sastre L.;
RT "Regulated expression of the MADS-box transcription factor SrfA mediates
RT activation of gene expression by protein kinase A during Dictyostelium
RT sporulation.";
RL Mech. Dev. 117:201-208(2002).
RN [5]
RP FUNCTION IN SPORE STABILITY, AND DISRUPTION PHENOTYPE.
RC STRAIN=AX4;
RX PubMed=14706699; DOI=10.1016/j.mod.2003.11.001;
RA Escalante R., Yamada Y., Cotter D., Sastre L., Sameshima M.;
RT "The MADS-box transcription factor SrfA is required for actin cytoskeleton
RT organization and spore coat stability during Dictyostelium sporulation.";
RL Mech. Dev. 121:51-56(2004).
CC -!- FUNCTION: Required for proper slug migration, spore differentiation and
CC stalk differentiation (under nonbuffered conditions). Could be involved
CC in late events of spore maturation necessary for spore stability.
CC {ECO:0000269|PubMed:14706699}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251}.
CC -!- DEVELOPMENTAL STAGE: Expressed in prestalk, prespore and spore cells.
CC Expression of mRNA starts at 8 hours of development when loose
CC aggregates form and remains constant until the onset of culmination at
CC 20 hours when a significant increase is observed.
CC {ECO:0000269|PubMed:11437439, ECO:0000269|PubMed:9729488}.
CC -!- INDUCTION: Induced by the protein kinase A (PKA) which is activated
CC during culmination. This leads to the expression of srfA in prespore
CC cells.
CC -!- DISRUPTION PHENOTYPE: Cells have an abnormal spore morphology and show
CC a greatly reduced spore viability. No defects in the overall morphology
CC of the structures were observed during development, except for a delay
CC of 3-4 hours in culmination. Prestalk gene expression is normal.
CC Expression of the prespore marker cotB is unaffected whereas expression
CC of the spore gene spiA is greatly reduced. Mutant spore show no
CC formation of mature actin rods. The spore outer layer gets wavier as
CC the spore ages and suffers a progressive degradation.
CC {ECO:0000269|PubMed:14706699, ECO:0000269|PubMed:9729488}.
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DR EMBL; AJ224893; CAA12197.1; -; Genomic_DNA.
DR EMBL; AAFI02000041; EAL66675.1; -; Genomic_DNA.
DR RefSeq; XP_640674.1; XM_635582.1.
DR AlphaFoldDB; Q54TY7; -.
DR SMR; Q54TY7; -.
DR STRING; 44689.DDB0214892; -.
DR PaxDb; Q54TY7; -.
DR EnsemblProtists; EAL66675; EAL66675; DDB_G0281387.
DR GeneID; 8623058; -.
DR KEGG; ddi:DDB_G0281387; -.
DR dictyBase; DDB_G0281387; srfA.
DR eggNOG; KOG0015; Eukaryota.
DR HOGENOM; CLU_657923_0_0_1; -.
DR InParanoid; Q54TY7; -.
DR OMA; NINGLDM; -.
DR Reactome; R-DDI-9031628; NGF-stimulated transcription.
DR PRO; PR:Q54TY7; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005634; C:nucleus; IC:dictyBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:dictyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0031247; P:actin rod assembly; IMP:dictyBase.
DR GO; GO:0031154; P:culmination involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0042331; P:phototaxis; IMP:dictyBase.
DR GO; GO:0043945; P:positive regulation of asexual sporulation resulting in formation of a cellular spore; IMP:dictyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:dictyBase.
DR GO; GO:0042244; P:spore wall assembly; IMP:dictyBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IMP:dictyBase.
DR CDD; cd00266; MADS_SRF_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033897; MADS_SRF-like.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..418
FT /note="Serum response factor homolog A"
FT /id="PRO_0000327938"
FT DOMAIN 67..127
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT REGION 14..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 143..170
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 301..351
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 388..418
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..40
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 305..351
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 388..412
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 253
FT /note="Q -> QQ (in Ref. 1; CAA12197)"
FT /evidence="ECO:0000305"
FT CONFLICT 343
FT /note="N -> NN (in Ref. 1; CAA12197)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 418 AA; 47546 MW; 8C86042A75C82D8A CRC64;
MSLSTVSNHI INPLGNVVYG NPSSPTSTSS SSSLTPTSTN EEMIKKEDSG TSEPSSPSTG
ECNGKKAGRR KIKIEFIDDK SRRHITFSKR KAGIMKKAYE LSTLTGTQVL LLVASETGHV
YTFATAKLQP LITRPEGKNL IQSCLNTPDN PNSPSMANQN SNNNSNLLQQ QQQQQLQQQQ
QLQQQQQQQQ QQQQQQQQQQ QQQQAAQQAV QQQQQQQAAQ QQAAQQQQLQ LQQQQQQQQQ
QQQQQQQQQQ QQQLQQLQQQ QLLQQQQHQQ IQQHQQQQQY INSNGYPETI YDIQDKDIER
RLGKDNNNNN NNNNNNINNN NNNNNSNNNS GNNNSNNNNN NNNKNNSNNN SQYIQQQNLN
IDLNYSNPNV YPIGNYFQQT VQSRYIQSSS SASSSPASPN QFNYSNHSMP LNNQFPPC
