SRFC_DICDI
ID SRFC_DICDI Reviewed; 1048 AA.
AC Q55F37;
DT 24-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 22-JAN-2014, sequence version 2.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Transcription factor mef2A;
DE AltName: Full=Myocyte enhancer factor 2A;
DE AltName: Full=Serum factor response C;
GN Name=mef2A; Synonyms=sfrC; ORFNames=DDB_G0268920;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP REVISION OF GENE MODEL, INDUCTION, DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=23577638; DOI=10.1186/1471-213x-13-12;
RA Galardi-Castilla M., Fernandez-Aguado I., Suarez T., Sastre L.;
RT "Mef2A, a homologue of animal Mef2 transcription factors, regulates cell
RT differentiation in Dictyostelium discoideum.";
RL BMC Dev. Biol. 13:12-12(2013).
CC -!- FUNCTION: Transcription factor that regulates cell differentiation
CC during development. Seems to negatively regulate prestalk gene
CC expression and positively regulate prespore gene expression.
CC {ECO:0000269|PubMed:23577638}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251}.
CC -!- INDUCTION: Induced during development in prespore cells.
CC {ECO:0000269|PubMed:23577638}.
CC -!- DISRUPTION PHENOTYPE: Leads to slower growth when feeded on bacteria,
CC fewer slugs with lower migration, decreased spore production, reduced
CC cell differentiation, and slightly abnormal prestalk differentiation.
CC {ECO:0000269|PubMed:23577638}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAL73049.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AAFI02000004; EAL73049.1; ALT_INIT; Genomic_DNA.
DR RefSeq; XP_646833.1; XM_641741.1.
DR AlphaFoldDB; Q55F37; -.
DR SMR; Q55F37; -.
DR STRING; 44689.DDB0220491; -.
DR PaxDb; Q55F37; -.
DR EnsemblProtists; EAL73049; EAL73049; DDB_G0268920.
DR GeneID; 8616515; -.
DR KEGG; ddi:DDB_G0268920; -.
DR dictyBase; DDB_G0268920; mef2A.
DR eggNOG; KOG0014; Eukaryota.
DR InParanoid; Q55F37; -.
DR PRO; PR:Q55F37; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0010468; P:regulation of gene expression; IMP:dictyBase.
DR GO; GO:1901261; P:regulation of sorocarp spore cell differentiation; IMP:dictyBase.
DR GO; GO:0031285; P:regulation of sorocarp stalk cell differentiation; IMP:dictyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00265; MADS_MEF2_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033896; MADS_MEF2-like.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Differentiation; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..1048
FT /note="Transcription factor mef2A"
FT /id="PRO_0000388785"
FT DOMAIN 1..61
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT REGION 74..263
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 294..339
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 386..812
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 916..1048
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 249..304
FT /evidence="ECO:0000255"
FT COMPBIAS 74..89
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 114..263
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 294..317
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 386..434
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 449..525
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 537..704
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 716..812
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 916..1033
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1034..1048
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1048 AA; 117228 MW; F92CC06D688788EB CRC64;
MGRNKITIEK ISNERNRQAT FTKRKNGLIK KAMELSILCD CEIAMIVFSS NNKLFQYSSR
DMDKLLIRYT DNTDNTRKNL TNQDYNRVFG NKKSKNDDED GDDDGDEDLG ETPTTPHGNL
NAHPIGSMEN NNNNNNNNNN NNNNNNNNIN NNNNNNNNSH HNNNNGNNNN NNNNNNNNNS
NNNSNNNNSN NGNSNNNNNG NNANHNMHHH NGNSANISPM QMSQQANNNN SNNNNNNGNN
NSNSNSNNNN NSNGYQQQQQ AAQQAVQQAQ MAQQMHLQQQ QQYQQLQHIQ QQQQQQHQQQ
QQNMGPNGYN HQQQQLQHQS PPPPPQQQQQ QMQHSQQQQN MNGIIGHHSP VIVGNGVSLL
QPSKWSASPY GEDLAPLTPR SASYKGIYGN QYPPQMQPVV NSNNNSNGNS NSMNNGISSP
PINQQNQQNQ KPPIMNKPGG GGQPMYYDYN GYPQQQQPPQ NYNSNGGYWG QNVSSPPPPQ
QQQSANPYIQ QQQQPQHQSP YYHGNYSPQQ QSPVLNSQNG HHSSPMHPHQ MHHQQHQHQQ
HPQMQQQQQQ QQQHQQHPQM QQIQQQQHPQ MQQHQQHQQQ HPQMQQQHMN NHQINHHHLN
SSPEINSQKN VHSSPLIMNS NNNNNNNNNN HHGNDNNNNN NNNSNNNNNN NNSNNNNNIN
NNINSNSNNN SNVNNGNNNN SGKVYNNNNN SNNGNNNNNK PEQTIPVKIE KEHSSSPTIP
EQPSINVSTS SNSAHVFNNI TSNSNNNNNN NNNNNNNNNN TNNNNNNNNN NNNNNNTNNI
NSNNNSSNTN VNVINNSSSS STPPISPANI NTQSPAISPA LVPVVTSLNP QGEETSKPKF
KKSLNLSIVI PEVGNSKYPP PQCKLTPINV EATKQPDIPT LPSPRDFYPE INLHHDNVSL
TPNYWGGWHQ TPRGSLLLTG NGGSNNSNSS NNNNNNNNNN NNTNNNNISG NGSSSSSSSS
TQNPNGFLGC LSPRSFNFLN ENNNNNNNNS SSGNNNNNNN NNNNNNNNNN NNNNNNNNNN
NNNNNNNSNP DESDQSFNRK RKSMEPKN
