SRF_DROME
ID SRF_DROME Reviewed; 449 AA.
AC Q24535; Q9W182;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Serum response factor homolog;
DE Short=dSRF;
DE AltName: Full=Protein blistered;
GN Name=bs; Synonyms=Serf; ORFNames=CG3411;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RX PubMed=7600954; DOI=10.1242/dev.120.4.743;
RA Affolter M., Montagne J., Walldorf U., Groppe J.C., Kloter U., Larosa M.,
RA Gehring W.J.;
RT "The Drosophila SRF homolog is expressed in a subset of tracheal cells and
RT maps within a genomic region required for tracheal development.";
RL Development 120:743-753(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=8787734; DOI=10.1242/dev.122.9.2589;
RA Montagne J., Groppe J., Guillemin K., Krasnow M.A., Gehring W.J.,
RA Affolter M.;
RT "The Drosophila serum response factor gene is required for the formation of
RT intervein tissue of the wing and is allelic to blistered.";
RL Development 122:2589-2597(1996).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=10940622; DOI=10.1016/s0925-4773(00)00373-7;
RA Nussbaumer U., Halder G., Groppe J., Affolter M., Montagne J.;
RT "Expression of the blistered/DSRF gene is controlled by different
RT morphogens during Drosophila trachea and wing development.";
RL Mech. Dev. 96:27-36(2000).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-156, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Required for the formation of intervein tissue of the wing.
CC Acts in a dosage-dependent manner to suppress wing vein formation and
CC promote development of intervein cells. Might play a role in the proper
CC formation and maintenance of the trachea. {ECO:0000269|PubMed:7600954,
CC ECO:0000269|PubMed:8787734}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251}.
CC -!- TISSUE SPECIFICITY: After germ band retraction, high levels of zygotic
CC expression are observed in a distinct subset of peripheral tracheal
CC cells distributed throughout the embryo and low levels in somatic
CC muscle. Expressed in the future intervein tissue of the wing imaginal
CC disk from the third instar larvae until eclosion of the adult fly (at
CC protein level). {ECO:0000269|PubMed:10940622,
CC ECO:0000269|PubMed:8787734}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC {ECO:0000269|PubMed:7600954}.
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DR EMBL; X77532; CAA54670.1; -; mRNA.
DR EMBL; AE013599; AAF47195.1; -; Genomic_DNA.
DR EMBL; AY095071; AAM11399.1; -; mRNA.
DR PIR; S42825; S42825.
DR RefSeq; NP_001286837.1; NM_001299908.1.
DR RefSeq; NP_726438.1; NM_166667.3.
DR AlphaFoldDB; Q24535; -.
DR SMR; Q24535; -.
DR BioGRID; 63464; 36.
DR IntAct; Q24535; 1.
DR STRING; 7227.FBpp0072178; -.
DR iPTMnet; Q24535; -.
DR PaxDb; Q24535; -.
DR DNASU; 37890; -.
DR EnsemblMetazoa; FBtr0072271; FBpp0072178; FBgn0004101.
DR EnsemblMetazoa; FBtr0343314; FBpp0309973; FBgn0004101.
DR GeneID; 37890; -.
DR KEGG; dme:Dmel_CG3411; -.
DR CTD; 37890; -.
DR FlyBase; FBgn0004101; bs.
DR VEuPathDB; VectorBase:FBgn0004101; -.
DR eggNOG; KOG0015; Eukaryota.
DR HOGENOM; CLU_045186_0_0_1; -.
DR InParanoid; Q24535; -.
DR OMA; ANIPPKM; -.
DR PhylomeDB; Q24535; -.
DR Reactome; R-DME-5663220; RHO GTPases Activate Formins.
DR SignaLink; Q24535; -.
DR BioGRID-ORCS; 37890; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 37890; -.
DR PRO; PR:Q24535; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0004101; Expressed in egg cell and 32 other tissues.
DR ExpressionAtlas; Q24535; baseline and differential.
DR Genevisible; Q24535; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0140297; F:DNA-binding transcription factor binding; IPI:FlyBase.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0007475; P:apposition of dorsal and ventral imaginal disc-derived wing surfaces; IMP:FlyBase.
DR GO; GO:0060446; P:branching involved in open tracheal system development; IMP:FlyBase.
DR GO; GO:0030154; P:cell differentiation; IMP:UniProtKB.
DR GO; GO:0007476; P:imaginal disc-derived wing morphogenesis; IMP:UniProtKB.
DR GO; GO:0008586; P:imaginal disc-derived wing vein morphogenesis; IMP:FlyBase.
DR GO; GO:0002168; P:instar larval development; IMP:UniProtKB.
DR GO; GO:0007424; P:open tracheal system development; IEP:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0035209; P:pupal development; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007614; P:short-term memory; IMP:FlyBase.
DR GO; GO:0030431; P:sleep; IMP:FlyBase.
DR GO; GO:0007430; P:terminal branching, open tracheal system; IMP:FlyBase.
DR GO; GO:0035220; P:wing disc development; IMP:UniProtKB.
DR CDD; cd00266; MADS_SRF_like; 1.
DR Gene3D; 3.40.1810.10; -; 1.
DR InterPro; IPR033897; MADS_SRF-like.
DR InterPro; IPR002100; TF_MADSbox.
DR InterPro; IPR036879; TF_MADSbox_sf.
DR Pfam; PF00319; SRF-TF; 1.
DR PRINTS; PR00404; MADSDOMAIN.
DR SMART; SM00432; MADS; 1.
DR SUPFAM; SSF55455; SSF55455; 1.
DR PROSITE; PS00350; MADS_BOX_1; 1.
DR PROSITE; PS50066; MADS_BOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Developmental protein; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..449
FT /note="Serum response factor homolog"
FT /id="PRO_0000199426"
FT DOMAIN 167..225
FT /note="MADS-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT REGION 23..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 270..360
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 418..449
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 70..112
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 271..286
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 318..358
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 418..433
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 156
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 17
FT /note="S -> R (in Ref. 1; CAA54670)"
FT /evidence="ECO:0000305"
FT CONFLICT 312
FT /note="S -> SS (in Ref. 1; CAA54670)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 449 AA; 47621 MW; BB9C52FA6BF35D06 CRC64;
MDPTRGDSRY NLNYSMSSIG LSLADPADMY GNPALGAGRP PSGGLLQNMG GVPPMQRPNP
AAGAPPAPQC QTLHSPQHAS QQQQQQQQQQ QQHQQQQQQQ QQHPQQQRGL KRSGSDCYED
HHRSSGGLTL QGLDNVASGA VDDSVDNYNP LPNKKSPPAN GKKTKGRVKI KMEYIDNKLR
RYTTFSKRKT GIMKKAYELS TLTGTQVMLL VASETGHVYT FATRKLQPMI TSEAGKQLIQ
TCLNSPDPPS VGGGDQRMSA TGFEETELSY NIADEDSKDD RSPTSSGNES DDSSDVEMPA
EAAEVATKLP ASKTEVSAPP AASCSAATAS SGHKTMPALN YQTDTNSGPS TSTAAGGGGS
ADSKYVYSAA SIANIPQKML RQLIQSGHLQ VHAEEDGNQY VTIPLSSTAA NLIKSNKLTA
SGSGASGSGT PVKNDASADK PLTIKQEFD
