SRGEF_MOUSE
ID SRGEF_MOUSE Reviewed; 464 AA.
AC Q80YD6; Q0VDT0; Q3UGM8; Q3UKY0; Q9QXB7;
DT 31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2003, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Secretion-regulating guanine nucleotide exchange factor;
DE AltName: Full=Deafness locus-associated putative guanine nucleotide exchange factor;
DE Short=DelGEF;
DE AltName: Full=Guanine nucleotide exchange factor-related protein;
GN Name=Sergef; Synonyms=Delgef, Gnefr;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10571079; DOI=10.1016/s0014-5793(99)01333-2;
RA Uhlmann J., Wiemann S., Ponstingl H.;
RT "DelGEF, an RCC1-related protein encoded by a gene on chromosome 11p14
RT critical for two forms of hereditary deafness.";
RL FEBS Lett. 460:153-160(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Melanocyte, and Placenta;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Probable guanine nucleotide exchange factor (GEF), which may
CC be involved in the secretion process.
CC -!- SUBUNIT: Interacts with SEC5. The interaction occurs only in the
CC presence of magnesium or manganese and is stimulated by dCTP or GTP (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
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DR EMBL; AJ243952; CAB60840.1; -; mRNA.
DR EMBL; AK145819; BAE26671.1; -; mRNA.
DR EMBL; AK147848; BAE28179.1; -; mRNA.
DR EMBL; BC049603; AAH49603.2; -; mRNA.
DR EMBL; BC119533; AAI19534.1; -; mRNA.
DR EMBL; BC119534; AAI19535.1; -; mRNA.
DR CCDS; CCDS21279.1; -.
DR RefSeq; NP_038817.1; NM_013789.2.
DR AlphaFoldDB; Q80YD6; -.
DR SMR; Q80YD6; -.
DR STRING; 10090.ENSMUSP00000033127; -.
DR PhosphoSitePlus; Q80YD6; -.
DR EPD; Q80YD6; -.
DR MaxQB; Q80YD6; -.
DR PaxDb; Q80YD6; -.
DR PRIDE; Q80YD6; -.
DR ProteomicsDB; 257399; -.
DR Antibodypedia; 12179; 102 antibodies from 22 providers.
DR DNASU; 27414; -.
DR Ensembl; ENSMUST00000033127; ENSMUSP00000033127; ENSMUSG00000030839.
DR GeneID; 27414; -.
DR KEGG; mmu:27414; -.
DR UCSC; uc009gyp.2; mouse.
DR CTD; 26297; -.
DR MGI; MGI:1351630; Sergef.
DR VEuPathDB; HostDB:ENSMUSG00000030839; -.
DR eggNOG; KOG1426; Eukaryota.
DR GeneTree; ENSGT00940000160684; -.
DR HOGENOM; CLU_005210_0_3_1; -.
DR InParanoid; Q80YD6; -.
DR OMA; GYESELC; -.
DR OrthoDB; 1062377at2759; -.
DR PhylomeDB; Q80YD6; -.
DR TreeFam; TF330842; -.
DR BioGRID-ORCS; 27414; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Sergef; mouse.
DR PRO; PR:Q80YD6; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q80YD6; protein.
DR Bgee; ENSMUSG00000030839; Expressed in dentate gyrus of hippocampal formation granule cell and 206 other tissues.
DR ExpressionAtlas; Q80YD6; baseline and differential.
DR Genevisible; Q80YD6; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; ISA:MGI.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0050709; P:negative regulation of protein secretion; ISO:MGI.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR Gene3D; 2.130.10.30; -; 2.
DR InterPro; IPR009091; RCC1/BLIP-II.
DR InterPro; IPR000408; Reg_chr_condens.
DR Pfam; PF00415; RCC1; 3.
DR PRINTS; PR00633; RCCNDNSATION.
DR SUPFAM; SSF50985; SSF50985; 1.
DR PROSITE; PS00626; RCC1_2; 2.
DR PROSITE; PS50012; RCC1_3; 7.
PE 2: Evidence at transcript level;
KW Cytoplasm; Guanine-nucleotide releasing factor; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat.
FT CHAIN 1..464
FT /note="Secretion-regulating guanine nucleotide exchange
FT factor"
FT /id="PRO_0000206650"
FT REPEAT 15..67
FT /note="RCC1 1"
FT REPEAT 68..119
FT /note="RCC1 2"
FT REPEAT 120..171
FT /note="RCC1 3"
FT REPEAT 172..230
FT /note="RCC1 4"
FT REPEAT 231..283
FT /note="RCC1 5"
FT REPEAT 284..351
FT /note="RCC1 6"
FT REPEAT 352..402
FT /note="RCC1 7"
FT REGION 422..464
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 428..446
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 429
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UGK8"
FT CONFLICT 448
FT /note="P -> Q (in Ref. 2; BAE26671)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 464 AA; 49226 MW; 2090CEEA0C914046 CRC64;
MARESCASET VSAAAVLFAW GANSYGQLGL GHKEDVFLPQ QLSDFCQAGC IKSVTGGGGH
SAVVTDGGDL FVCGLNKDGQ LGLGHTEEVL RFTICKPLRG CPIRQVACGW DFTIMLTEKG
QVLSCGSNAF GQLGVPHGPR KCVVPQAIEC LREKVVCVAA GLRHALATTA TGSVFQWGTG
LASSGRRLCP GQNLPLFLTA KEPSRVTGLE NSTAVCAVAG SDHSASLTDT GELYVWGRNK
HGQLASRAVF LPLPQRIEAH YFQDEKVTAV WSGWTHLVAK TETGKVFTWG RADYGQLGRR
LEPPEAQKPV EQDSSLAFQG PQNSVPSPLH CLTGATEISC GSEHNLAVIR DKCCSWGWNE
HGMCGDGTES NVWTPTPVQA LPPSPSRLLL VGCGAGHSLA VCQLPAHPVP CQDLKVTCPL
PDDTENTESQ GAVDRDRLEG ETISDLNPDR TRNGGGGCES ETVQ
