SRK2_SPOLA
ID SRK2_SPOLA Reviewed; 362 AA.
AC P42688;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Tyrosine-protein kinase SRK2;
DE EC=2.7.10.2;
DE Flags: Fragment;
GN Name=SRK2;
OS Spongilla lacustris (Freshwater sponge).
OC Eukaryota; Metazoa; Porifera; Demospongiae; Heteroscleromorpha;
OC Spongillida; Spongillidae; Spongilla.
OX NCBI_TaxID=6055;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1378585;
RA Ottilie S., Raulf F., Barnekow A., Hannig G., Schartl M.;
RT "Multiple src-related kinase genes, srk1-4, in the fresh water sponge
RT Spongilla lacustris.";
RL Oncogene 7:1625-1630(1992).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-
CC [protein]; Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC COMP:10137, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858,
CC ChEBI:CHEBI:82620, ChEBI:CHEBI:456216; EC=2.7.10.2;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10028};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
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DR EMBL; X61602; CAA43799.1; -; mRNA.
DR PIR; S24551; S24551.
DR AlphaFoldDB; P42688; -.
DR SMR; P42688; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.30.505.10; -; 1.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR InterPro; IPR000980; SH2.
DR InterPro; IPR036860; SH2_dom_sf.
DR InterPro; IPR008266; Tyr_kinase_AS.
DR InterPro; IPR020635; Tyr_kinase_cat_dom.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR Pfam; PF00017; SH2; 1.
DR PRINTS; PR00401; SH2DOMAIN.
DR PRINTS; PR00109; TYRKINASE.
DR SMART; SM00252; SH2; 1.
DR SMART; SM00219; TyrKc; 1.
DR SUPFAM; SSF55550; SSF55550; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00109; PROTEIN_KINASE_TYR; 1.
DR PROSITE; PS50001; SH2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cytoplasm; Kinase; Nucleotide-binding; Phosphoprotein;
KW SH2 domain; Transferase; Tyrosine-protein kinase.
FT CHAIN <1..362
FT /note="Tyrosine-protein kinase SRK2"
FT /id="PRO_0000088158"
FT DOMAIN <1..70
FT /note="SH2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00191"
FT DOMAIN 95..348
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT ACT_SITE 214
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10028"
FT BINDING 101..109
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 123
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT NON_TER 1
SQ SEQUENCE 362 AA; 40937 MW; D8C549E9904EB6D0 CRC64;
TFLVRESESK PGDYSLSIQD GDNVKHYRIR KLDEGGFFIT RRAVFNTLKD LVQYYQNESD
GLCVNLRQPC RPLERPQIDL SHKTKDMWEI SRDSITLIRK LGAGQFGEVY QGLWNNSTPV
AVKTLKAGTM QPAAFLAEAQ IMKKLRHPKL IQLYAVCTQG EPVYIITELM SKGSLLDYLQ
GEAGALKLPQ LIDMAAQVAA GMAYLELHNY IHRDLAARNI LVGDNNICKV ADFGLARLIV
SDDYNATEGA KFPIKWTAPE AALFNRFSIK SDVWSFGILI TELVTYGRIP YPGMSNAEVL
QNLDKGYRMP CPVTTPESLY QIMLDCWKRN PADRPTFEAL QWRLEDFFVL DAGQYQHAAD
VL
