SRK3_SPOLA
ID SRK3_SPOLA Reviewed; 334 AA.
AC P42689;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Tyrosine-protein kinase SRK3;
DE EC=2.7.10.2;
DE Flags: Fragment;
GN Name=SRK3;
OS Spongilla lacustris (Freshwater sponge).
OC Eukaryota; Metazoa; Porifera; Demospongiae; Heteroscleromorpha;
OC Spongillida; Spongillidae; Spongilla.
OX NCBI_TaxID=6055;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1378585;
RA Ottilie S., Raulf F., Barnekow A., Hannig G., Schartl M.;
RT "Multiple src-related kinase genes, srk1-4, in the fresh water sponge
RT Spongilla lacustris.";
RL Oncogene 7:1625-1630(1992).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-
CC [protein]; Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC COMP:10137, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858,
CC ChEBI:CHEBI:82620, ChEBI:CHEBI:456216; EC=2.7.10.2;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10028};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
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DR EMBL; X61603; CAA43800.1; -; mRNA.
DR PIR; S24552; S24552.
DR AlphaFoldDB; P42689; -.
DR SMR; P42689; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.30.505.10; -; 1.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR InterPro; IPR000980; SH2.
DR InterPro; IPR036860; SH2_dom_sf.
DR InterPro; IPR008266; Tyr_kinase_AS.
DR InterPro; IPR020635; Tyr_kinase_cat_dom.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR PRINTS; PR00109; TYRKINASE.
DR SMART; SM00219; TyrKc; 1.
DR SUPFAM; SSF55550; SSF55550; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00109; PROTEIN_KINASE_TYR; 1.
DR PROSITE; PS50001; SH2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cytoplasm; Kinase; Nucleotide-binding; Phosphoprotein;
KW SH2 domain; Transferase; Tyrosine-protein kinase.
FT CHAIN <1..334
FT /note="Tyrosine-protein kinase SRK3"
FT /id="PRO_0000088159"
FT DOMAIN <1..42
FT /note="SH2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00191"
FT DOMAIN 66..321
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT ACT_SITE 186
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10028"
FT BINDING 72..80
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 94
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT NON_TER 1
SQ SEQUENCE 334 AA; 37880 MW; 2AFEB9C409817AA2 CRC64;
IRTLDDGGFY MANRISFPTL QNLVSHYMMD ADGLAQRLSR PCSRANIPIT SGLSYKDEWE
IDRTTIQLQR KLGQGNFGEV WAGVWNGTTA VAVKTLKPDT MEVKDFVQEA QVMKKIHHPN
LLQLYAVCTI GEPIYIVTEL MKYGSMLEYL KHGEGKNITL HQMVDMSAQI ASGMTYLEAH
SYIHRDLAAR NILVGEGNVC KVADFGLARV IKEDIYNPRE GTKFPIKWTA PEAALYNRFT
IKSDVWSFGV LISEIVTHGR MPYPGMTNRQ VLEAVDRGYR MPCPEGCPDP LYKIMLSCWK
HEPDDRPTFE SLKNLLEDYY VSAAEGSYRE PAPR
