SRL1_YEAST
ID SRL1_YEAST Reviewed; 210 AA.
AC Q08673; D6W2U9;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Cell wall protein SRL1;
DE Flags: Precursor;
GN Name=SRL1; OrderedLocusNames=YOR247W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9153759;
RX DOI=10.1002/(sici)1097-0061(199704)13:5<483::aid-yea105>3.0.co;2-u;
RA Poirey R., Jauniaux J.-C.;
RT "Sequencing analysis of a 36.8 kb fragment of yeast chromosome XV reveals
RT 26 open reading frames including SEC63, CDC31, SUG2, GCD1, RBL2, PNT1, PAC1
RT and VPH1.";
RL Yeast 13:483-487(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=11935221; DOI=10.1007/s00294-001-0264-9;
RA Terashima H., Fukuchi S., Nakai K., Arisawa M., Hamada K., Yabuki N.,
RA Kitada K.;
RT "Sequence-based approach for identification of cell wall proteins in
RT Saccharomyces cerevisiae.";
RL Curr. Genet. 40:311-316(2002).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=13679573; DOI=10.1073/pnas.2033246100;
RA Shepard K.A., Gerber A.P., Jambhekar A., Takizawa P.A., Brown P.O.,
RA Herschlag D., DeRisi J.L., Vale R.D.;
RT "Widespread cytoplasmic mRNA transport in yeast: identification of 22 bud-
RT localized transcripts using DNA microarray analysis.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:11429-11434(2003).
RN [7]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15165243; DOI=10.1111/j.1365-2958.2004.04064.x;
RA Hagen I., Ecker M., Lagorce A., Francois J.M., Sestak S., Rachel R.,
RA Grossmann G., Hauser N.C., Hoheisel J.D., Tanner W., Strahl S.;
RT "Sed1p and Srl1p are required to compensate for cell wall instability in
RT Saccharomyces cerevisiae mutants defective in multiple GPI-anchored
RT mannoproteins.";
RL Mol. Microbiol. 52:1413-1425(2004).
RN [8]
RP FUNCTION.
RX PubMed=15972461; DOI=10.1534/genetics.105.042101;
RA Kurischko C., Weiss G., Ottey M., Luca F.C.;
RT "A role for the Saccharomyces cerevisiae regulation of Ace2 and polarized
RT morphogenesis signaling network in cell integrity.";
RL Genetics 171:443-455(2005).
CC -!- FUNCTION: Required to stabilize the cell wall in the absence of
CC multiple GPI-anchored mannoproteins. {ECO:0000269|PubMed:15165243,
CC ECO:0000269|PubMed:15972461}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall. Cell surface.
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DR EMBL; Z75155; CAA99468.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA11015.1; -; Genomic_DNA.
DR PIR; S67140; S67140.
DR RefSeq; NP_014890.1; NM_001183666.1.
DR AlphaFoldDB; Q08673; -.
DR BioGRID; 34638; 54.
DR DIP; DIP-4789N; -.
DR IntAct; Q08673; 4.
DR MINT; Q08673; -.
DR STRING; 4932.YOR247W; -.
DR PaxDb; Q08673; -.
DR TopDownProteomics; Q08673; -.
DR EnsemblFungi; YOR247W_mRNA; YOR247W; YOR247W.
DR GeneID; 854421; -.
DR KEGG; sce:YOR247W; -.
DR SGD; S000005773; SRL1.
DR VEuPathDB; FungiDB:YOR247W; -.
DR eggNOG; ENOG502SV02; Eukaryota.
DR HOGENOM; CLU_1355342_0_0_1; -.
DR InParanoid; Q08673; -.
DR OMA; CAPVTEY; -.
DR BioCyc; YEAST:G3O-33740-MON; -.
DR PRO; PR:Q08673; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; Q08673; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR GO; GO:0005934; C:cellular bud tip; IDA:SGD.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0009277; C:fungal-type cell wall; IDA:SGD.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0031505; P:fungal-type cell wall organization; IMP:SGD.
DR GO; GO:0006139; P:nucleobase-containing compound metabolic process; IMP:SGD.
PE 1: Evidence at protein level;
KW Cell wall; Cell wall biogenesis/degradation; Glycoprotein;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..210
FT /note="Cell wall protein SRL1"
FT /id="PRO_0000270576"
FT REGION 80..99
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 118..142
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 23
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 174
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 200
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 206
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 210 AA; 22048 MW; EE5EF4D74B0EF306 CRC64;
MLQSVVFFAL LTFASSVSAI YSNNTVSTTT TLAPSYSLVP QETTISYADD TTTFFVTSTV
YSTSWFTSTS ATITNAASSS LSTSSASGSV TPESTHEITS TSTITSTLLL TLHDSTTLSP
SSTAASVSDE DSNNKDAKVK SFEQASTSNG CVPITKFVTV TNEPVTQYVT VTPNTTTQYV
TVTGAPSVTT TSPGNVQWYN TTSITNSTSW
