SRL3_YEAST
ID SRL3_YEAST Reviewed; 246 AA.
AC P36167; D6VXF1;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Protein SRL3;
DE AltName: Full=RAD53 lethality suppressor protein 3;
GN Name=SRL3; OrderedLocusNames=YKR091W; ORFNames=YKR411;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8154186; DOI=10.1002/yea.320091209;
RA Bou G., Esteban P.F., Baladron V., Gonzalez G.A., Cantalejo J.G.,
RA Remacha M.A., Jimenez A., del Rey F., Ballesta J.P.G., Revuelta J.L.;
RT "The complete sequence of a 15,820 bp segment of Saccharomyces cerevisiae
RT chromosome XI contains the UBI2 and MPL1 genes and three new open reading
RT frames.";
RL Yeast 9:1349-1354(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-246.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=11914276; DOI=10.1101/gad.970902;
RA Kumar A., Agarwal S., Heyman J.A., Matson S., Heidtman M., Piccirillo S.,
RA Umansky L., Drawid A., Jansen R., Liu Y., Cheung K.-H., Miller P.,
RA Gerstein M., Roeder G.S., Snyder M.;
RT "Subcellular localization of the yeast proteome.";
RL Genes Dev. 16:707-719(2002).
RN [6]
RP IDENTIFICATION OF FRAMESHIFT.
RX PubMed=12748633; DOI=10.1038/nature01644;
RA Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT "Sequencing and comparison of yeast species to identify genes and
RT regulatory elements.";
RL Nature 423:241-254(2003).
RN [7]
RP PHOSPHORYLATION BY CDC28.
RX PubMed=14574415; DOI=10.1038/nature02062;
RA Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K.,
RA Shokat K.M., Morgan D.O.;
RT "Targets of the cyclin-dependent kinase Cdk1.";
RL Nature 425:859-864(2003).
RN [8]
RP INTERACTION WITH CLN2, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=15200949; DOI=10.1016/j.molcel.2004.05.025;
RA Archambault V., Chang E.J., Drapkin B.J., Cross F.R., Chait B.T.,
RA Rout M.P.;
RT "Targeted proteomic study of the cyclin-Cdk module.";
RL Mol. Cell 14:699-711(2004).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-212, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-212, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Weakly suppresses a RAD53 null mutation when overexpressed.
CC -!- SUBUNIT: Interacts with CLN2. {ECO:0000269|PubMed:15200949}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11914276}.
CC -!- PTM: Phosphorylated by CDC28, probably in association with G1 cyclin
CC CLN2. {ECO:0000269|PubMed:14574415}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA51945.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA82170.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X73541; CAA51945.1; ALT_FRAME; Genomic_DNA.
DR EMBL; Z28316; CAA82170.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AY558318; AAS56644.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09241.1; -; Genomic_DNA.
DR PIR; S38169; S38169.
DR RefSeq; NP_013017.2; NM_001179881.1.
DR AlphaFoldDB; P36167; -.
DR BioGRID; 34222; 167.
DR DIP; DIP-4750N; -.
DR IntAct; P36167; 7.
DR MINT; P36167; -.
DR STRING; 4932.YKR091W; -.
DR iPTMnet; P36167; -.
DR MaxQB; P36167; -.
DR PaxDb; P36167; -.
DR PRIDE; P36167; -.
DR EnsemblFungi; YKR091W_mRNA; YKR091W; YKR091W.
DR GeneID; 853966; -.
DR KEGG; sce:YKR091W; -.
DR SGD; S000001799; SRL3.
DR VEuPathDB; FungiDB:YKR091W; -.
DR HOGENOM; CLU_1070206_0_0_1; -.
DR InParanoid; P36167; -.
DR OMA; WSSHRRT; -.
DR BioCyc; YEAST:G3O-32054-MON; -.
DR PRO; PR:P36167; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P36167; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0033309; C:SBF transcription complex; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:SGD.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0071930; P:negative regulation of transcription involved in G1/S transition of mitotic cell cycle; IEA:InterPro.
DR GO; GO:0006139; P:nucleobase-containing compound metabolic process; IMP:SGD.
DR InterPro; IPR039198; Srl3/Whi5.
DR PANTHER; PTHR28246; PTHR28246; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..246
FT /note="Protein SRL3"
FT /id="PRO_0000203228"
FT REGION 49..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 200..229
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 202..229
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 212
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
SQ SEQUENCE 246 AA; 27466 MW; C305AAC89310E65D CRC64;
MFLKTPNWET VNETPKSRVL TINELISPNL DTESNSLLAT PARKYFKTSI SEAQDSPTSA
PSPDGNEDPT YQYNVQFHFP GPITPTTPRS KNAEMFPSPT PPLVSPTAVI EEENDDSVRE
FSRTLKSRLN CAMVKLSKEH EQVALIPPPP TEKIRKGSYS NKFAAKHRRC HSLDESKKFL
SSLEDSSAHA AFLKAISSKH AKSNRVDNVN VSPLRWSSHR RTQSTQENSL QEVVAIDTLL
KMSSSD
