SRL4_YEAST
ID SRL4_YEAST Reviewed; 281 AA.
AC Q03085; D6W3Y0;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Oxidoreductase-like protein SRL4;
DE AltName: Full=Suppressor of RAD53 and LCD1 4;
DE AltName: Full=Suppressor of RAD53 null lethality 4;
GN Name=SRL4; OrderedLocusNames=YPL033C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION.
RX PubMed=10051619; DOI=10.1073/pnas.96.5.2204;
RA Perkins E.L., Sterling J.F., Hashem V.I., Resnick M.A.;
RT "Yeast and human genes that affect the Escherichia coli SOS response.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:2204-2209(1999).
RN [4]
RP INDUCTION.
RX PubMed=17101777; DOI=10.1128/mcb.00439-06;
RA Lahav R., Gammie A., Tavazoie S., Rose M.D.;
RT "Role of transcription factor Kar4 in regulating downstream events in the
RT Saccharomyces cerevisiae pheromone response pathway.";
RL Mol. Cell. Biol. 27:818-829(2007).
RN [5]
RP DISRUPTION PHENOTYPE.
RX PubMed=18337697; DOI=10.1007/s12275-008-0013-6;
RA Choi D.H., Oh Y.M., Kwon S.H., Bae S.H.;
RT "The mutation of a novel Saccharomyces cerevisiae SRL4 gene rescues the
RT lethality of rad53 and lcd1 mutations by modulating dNTP levels.";
RL J. Microbiol. 46:75-80(2008).
CC -!- FUNCTION: May be involved in the regulation of dNTP production. Induces
CC the SOS system when expressed in E.coli, therefore, it may play a role
CC in DNA metabolism and/or in genome stability.
CC {ECO:0000269|PubMed:10051619}.
CC -!- INDUCTION: Expression is induced by the KAR4 transcription factor.
CC {ECO:0000269|PubMed:17101777}.
CC -!- DISRUPTION PHENOTYPE: Suppresses the lethality of LCD1 and RAD53
CC mutations. Leads to resistance to the chemicals that inhibit nucleotide
CC metabolism and increases dNTP levels in the presence of hydroxyurea.
CC {ECO:0000269|PubMed:18337697}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
CC -!- CAUTION: Related to oxidoreductases, but misses the conserved Thr
CC active site. Therefore it may not have any oxidoreductase activity.
CC {ECO:0000305}.
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DR EMBL; U44030; AAB68186.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11396.1; -; Genomic_DNA.
DR PIR; S62041; S62041.
DR RefSeq; NP_015292.1; NM_001183847.1.
DR AlphaFoldDB; Q03085; -.
DR SMR; Q03085; -.
DR BioGRID; 36145; 49.
DR IntAct; Q03085; 1.
DR STRING; 4932.YPL033C; -.
DR iPTMnet; Q03085; -.
DR PaxDb; Q03085; -.
DR EnsemblFungi; YPL033C_mRNA; YPL033C; YPL033C.
DR GeneID; 856074; -.
DR KEGG; sce:YPL033C; -.
DR SGD; S000005954; SRL4.
DR VEuPathDB; FungiDB:YPL033C; -.
DR eggNOG; ENOG502S2NP; Eukaryota.
DR HOGENOM; CLU_064112_0_0_1; -.
DR InParanoid; Q03085; -.
DR OMA; ESEAHWK; -.
DR BioCyc; YEAST:G3O-33948-MON; -.
DR Reactome; R-SCE-193144; Estrogen biosynthesis.
DR Reactome; R-SCE-2187335; The retinoid cycle in cones (daylight vision).
DR Reactome; R-SCE-5365859; RA biosynthesis pathway.
DR Reactome; R-SCE-8964572; Lipid particle organization.
DR PRO; PR:Q03085; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q03085; protein.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0009200; P:deoxyribonucleoside triphosphate metabolic process; IMP:SGD.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 2: Evidence at transcript level;
KW Reference proteome.
FT CHAIN 1..281
FT /note="Oxidoreductase-like protein SRL4"
FT /id="PRO_0000269762"
SQ SEQUENCE 281 AA; 31925 MW; 24207F3D054DE5B2 CRC64;
MKKTIYKVLV SFYQYVGLGK KFHPSHDTVL IIGGSSNELG IELCETFIED YHTKVINIDT
IDSINGKNAR RSEKLYTFIS CKDFSDIKCL EESMLYLQNL EIIPTVLINN MQEGIESTLL
KEDKFLRLDE ESLNEFEKIV RYNLQSVILI TKFCLSNIFP KVQAEAQEKA KGFYIVNISS
VLTLKPCKSG THFITSKCGI NSFHDGITSE LKLKDSNLNV KTLIAYLPSF ESEAHWKRLS
PSISKHLVHC LLEGRYGDTI LESKRSIGDI LLITGFKSSF T
