SRMB_HAEIN
ID SRMB_HAEIN Reviewed; 439 AA.
AC P44701;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=ATP-dependent RNA helicase SrmB {ECO:0000255|HAMAP-Rule:MF_00967};
DE EC=3.6.4.13 {ECO:0000255|HAMAP-Rule:MF_00967};
GN Name=srmB {ECO:0000255|HAMAP-Rule:MF_00967}; OrderedLocusNames=HI_0422;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
CC -!- FUNCTION: DEAD-box RNA helicase involved in the assembly of the 50S
CC ribosomal subunit at low temperature. Exhibits RNA-stimulated ATP
CC hydrolysis and RNA unwinding activity. {ECO:0000255|HAMAP-
CC Rule:MF_00967}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00967};
CC -!- SUBUNIT: Interacts with the 50S ribosomal subunit. {ECO:0000255|HAMAP-
CC Rule:MF_00967}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00967}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. SrmB subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00967}.
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DR EMBL; L42023; AAC22078.1; -; Genomic_DNA.
DR PIR; H64066; H64066.
DR RefSeq; NP_438583.1; NC_000907.1.
DR RefSeq; WP_005693742.1; NC_000907.1.
DR AlphaFoldDB; P44701; -.
DR SMR; P44701; -.
DR STRING; 71421.HI_0422; -.
DR EnsemblBacteria; AAC22078; AAC22078; HI_0422.
DR KEGG; hin:HI_0422; -.
DR PATRIC; fig|71421.8.peg.442; -.
DR eggNOG; COG0513; Bacteria.
DR HOGENOM; CLU_003041_28_3_6; -.
DR OMA; YDIELYQ; -.
DR PhylomeDB; P44701; -.
DR BioCyc; HINF71421:G1GJ1-437-MON; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000027; P:ribosomal large subunit assembly; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 2.
DR HAMAP; MF_00967; DEAD_helicase_SrmB; 1.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR028621; DEAD_helicase_SrmB.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; Helicase; Hydrolase; Nucleotide-binding;
KW Reference proteome; Ribosome biogenesis.
FT CHAIN 1..439
FT /note="ATP-dependent RNA helicase SrmB"
FT /id="PRO_0000055110"
FT DOMAIN 35..209
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00967"
FT DOMAIN 237..387
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00967"
FT REGION 381..439
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 4..32
FT /note="Q motif"
FT MOTIF 157..160
FT /note="DEAD box"
FT COMPBIAS 381..414
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 415..429
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 48..55
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00967"
SQ SEQUENCE 439 AA; 49805 MW; 7543942CE35B20C4 CRC64;
MNLSQFEQFD LSPELLKALE KKGYSRPTAI QMEAIPAAME ESDVLGSAPT GTGKTAAFLL
PALQHLLDYP RRKPGPPRIL VLTPTRELAM QVAEQAEELA QFTHLNIATI TGGVAYQNHG
DVFNTNQDLV VATPGRLLQY IKEENFDCRS VEMLIFDEAD RMLQMGFGQD AEKIAAETRW
RKQTLLFSAT LEGELLVDFA ERLLNDPVKV DAEPSRRERK KINQWYYHAD SNEHKIKLLA
RFIETEEVTR GIVFIRRRED ARELSETLRK RGIRSAYLEG EMAQTQRNNA IDKLKSGIVT
VLVATDVAAR GIDIDDVSHV MNFDLPYSAD TYLHRIGRTA RAGKKGTAVS FVEAHDYKLL
GKIKRYTEEI LKARILAGLE PRTKPPKDGE VKSVSKKQKA RIKEKREEKK KTEAKKKVKL
RHKDTKNIGK RRKPSNSNV
