SRNB_ECOLI
ID SRNB_ECOLI Reviewed; 68 AA.
AC P13970;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1990, sequence version 1.
DT 25-MAY-2022, entry version 108.
DE RecName: Full=Protein SrnB;
GN Name=srnB; OrderedLocusNames=ECOK12F004/ECOK12F005;
OS Escherichia coli (strain K12).
OG Plasmid F.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3032897; DOI=10.1128/jb.169.5.1836-1846.1987;
RA Saadi S., Maas W.K., Hill D.F., Bergquist P.L.;
RT "Nucleotide sequence analysis of RepFIC, a basic replicon present in IncFI
RT plasmids P307 and F, and its relation to the RepA replicon of IncFII
RT plasmids.";
RL J. Bacteriol. 169:1836-1846(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=YS12;
RA Akimoto S., Ono K., Ono T., Ohnishi Y.;
RT "Nucleotide sequence of the F plasmid gene srnB that promotes degradation
RT of stable RNA in Escherichia coli.";
RL FEMS Microbiol. Lett. 33:241-245(1986).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / CR63;
RA Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT "Complete nucleotide sequence of the F plasmid: its implications for
RT organization and diversification of plasmid genomes.";
RL Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Toxic component of a type I toxin-antitoxin (TA) system (By
CC similarity). Its normal function is believed to be effective plasmid
CC stabilization through postsegregational killing of cells that have lost
CC the F plasmid. Promotes degradation of stable RNA in E.coli (Ref.2).
CC {ECO:0000250|UniProtKB:P0ACG4, ECO:0000305|Ref.2}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P0ACG4}; Single-pass membrane protein
CC {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=srnB;
CC IsoId=P13970-1; Sequence=Displayed;
CC Name=srnB';
CC IsoId=P13970-2; Sequence=VSP_018748;
CC -!- SIMILARITY: Belongs to the Hok/Gef family. {ECO:0000305}.
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DR EMBL; M16168; AAA99006.1; -; Genomic_DNA.
DR EMBL; X14442; CAA32614.1; -; Genomic_DNA.
DR EMBL; M35279; AAA98078.1; -; Genomic_DNA.
DR EMBL; AP001918; BAA97874.1; -; Genomic_DNA.
DR EMBL; AP001918; BAA97875.1; -; Genomic_DNA.
DR PIR; C26870; C26870.
DR RefSeq; NP_061383.1; NC_002483.1. [P13970-1]
DR RefSeq; NP_061384.1; NC_002483.1. [P13970-2]
DR RefSeq; WP_001362723.1; NC_002483.1.
DR AlphaFoldDB; P13970; -.
DR SMR; P13970; -.
DR TCDB; 1.E.53.1.11; the toxic hok/gef protein (hok/gef) family.
DR PRIDE; P13970; -.
DR PATRIC; fig|83333.107.peg.599; -.
DR PRO; PR:P13970; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR000021; Hok/gef_toxin.
DR InterPro; IPR018084; Hok/gef_toxin_CS.
DR Pfam; PF01848; HOK_GEF; 1.
DR PRINTS; PR00281; HOKGEFTOXIC.
DR PROSITE; PS00556; HOK_GEF; 1.
PE 3: Inferred from homology;
KW Alternative initiation; Cell inner membrane; Cell membrane; Membrane;
KW Plasmid; Toxin-antitoxin system; Transmembrane; Transmembrane helix.
FT CHAIN 1..68
FT /note="Protein SrnB"
FT /id="PRO_0000013487"
FT TRANSMEM 23..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..19
FT /note="Missing (in isoform srnB')"
FT /evidence="ECO:0000305"
FT /id="VSP_018748"
SQ SEQUENCE 68 AA; 7792 MW; F6E4F31EE4BB93D6 CRC64;
MKYLNTTDCS LFLAERSKFM TKYALIGLLA VCATVLCFSL IFRERLCELN IHRGNTVVQV
TLAYEARK
