SRP14_ARATH
ID SRP14_ARATH Reviewed; 121 AA.
AC O04421; O22839; Q53YG7;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 2.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Signal recognition particle 14 kDa protein;
DE Short=SRP14;
GN Name=SRP14; OrderedLocusNames=At2g43640; ORFNames=F18O19.25;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia; TISSUE=Root;
RA Bui N., Wolff N., Strub K.;
RL Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the signal recognition particle (SRP) complex, a
CC ribonucleoprotein complex that mediates the cotranslational targeting
CC of secretory and membrane proteins to the endoplasmic reticulum (ER)
CC (By similarity). SRP9 together with SRP14 and the Alu portion of the
CC SRP RNA, constitutes the elongation arrest domain of SRP (By
CC similarity). The complex of SRP9 and SRP14 is required for SRP RNA
CC binding (By similarity). {ECO:0000250|UniProtKB:P37108}.
CC -!- SUBUNIT: Heterodimer with SRP9; binds RNA as heterodimer (By
CC similarity). Component of a signal recognition particle (SRP) complex
CC that consists of a 7SL RNA molecule of 300 nucleotides and six protein
CC subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9 (By similarity).
CC {ECO:0000250|UniProtKB:P16255, ECO:0000250|UniProtKB:P37108}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=O04421-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the SRP14 family. {ECO:0000305}.
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DR EMBL; Y10116; CAA71202.1; -; mRNA.
DR EMBL; AC002333; AAB64042.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10298.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10299.1; -; Genomic_DNA.
DR EMBL; BT003088; AAO23653.1; -; mRNA.
DR EMBL; AK227403; BAE99407.1; -; mRNA.
DR EMBL; AY088850; AAM67157.1; -; mRNA.
DR PIR; F84868; F84868.
DR RefSeq; NP_001078050.1; NM_001084581.2. [O04421-1]
DR RefSeq; NP_181892.1; NM_129926.2. [O04421-1]
DR AlphaFoldDB; O04421; -.
DR SMR; O04421; -.
DR BioGRID; 4302; 2.
DR IntAct; O04421; 2.
DR STRING; 3702.AT2G43640.1; -.
DR PaxDb; O04421; -.
DR PRIDE; O04421; -.
DR ProteomicsDB; 226736; -. [O04421-1]
DR EnsemblPlants; AT2G43640.1; AT2G43640.1; AT2G43640. [O04421-1]
DR EnsemblPlants; AT2G43640.2; AT2G43640.2; AT2G43640. [O04421-1]
DR GeneID; 818966; -.
DR Gramene; AT2G43640.1; AT2G43640.1; AT2G43640. [O04421-1]
DR Gramene; AT2G43640.2; AT2G43640.2; AT2G43640. [O04421-1]
DR KEGG; ath:AT2G43640; -.
DR Araport; AT2G43640; -.
DR TAIR; locus:2044029; AT2G43640.
DR eggNOG; KOG1761; Eukaryota.
DR HOGENOM; CLU_094309_2_0_1; -.
DR InParanoid; O04421; -.
DR OMA; RFNGHNK; -.
DR PhylomeDB; O04421; -.
DR PRO; PR:O04421; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O04421; baseline and differential.
DR Genevisible; O04421; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005786; C:signal recognition particle, endoplasmic reticulum targeting; IBA:GO_Central.
DR GO; GO:0008312; F:7S RNA binding; IEA:InterPro.
DR GO; GO:0030942; F:endoplasmic reticulum signal peptide binding; IEA:InterPro.
DR GO; GO:0045047; P:protein targeting to ER; IBA:GO_Central.
DR GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IEA:InterPro.
DR Gene3D; 3.30.720.10; -; 1.
DR InterPro; IPR003210; Signal_recog_particle_SRP14.
DR InterPro; IPR009018; Signal_recog_particle_SRP9/14.
DR PANTHER; PTHR12013; PTHR12013; 1.
DR Pfam; PF02290; SRP14; 1.
DR SUPFAM; SSF54762; SSF54762; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Reference proteome; Ribonucleoprotein;
KW RNA-binding; Signal recognition particle.
FT CHAIN 1..121
FT /note="Signal recognition particle 14 kDa protein"
FT /id="PRO_0000135193"
FT REGION 93..121
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 99..121
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 116
FT /note="T -> P (in Ref. 1; CAA71202)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 121 AA; 13781 MW; 216D2AA83B2227DD CRC64;
MVLLQLDPFL NELTSMFEKS KEKGSVWVTL KRSSLKSKVQ KRKLSSVGES IEYRCLIRAT
DGKKTVSTSV GAKDHQRFQA SYATILKAHM TALKKRERKD RKKSTEAEKK ESTSTTKSKK
L
