SRP21_YEAST
ID SRP21_YEAST Reviewed; 167 AA.
AC P32342; D6VXG8;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Signal recognition particle subunit SRP21;
DE AltName: Full=Signal recognition particle 21 kDa protein;
GN Name=SRP21; OrderedLocusNames=YKL122C; ORFNames=YKL527;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1514329; DOI=10.1002/yea.320080410;
RA Colleaux L., Richard G.-F., Thierry A., Dujon B.;
RT "Sequence of a segment of yeast chromosome XI identifies a new
RT mitochondrial carrier, a new member of the G protein family, and a protein
RT with the PAAKK motif of the H1 histones.";
RL Yeast 8:325-336(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP PROTEIN SEQUENCE OF 2-15; 45-55; 75-85 AND 107-120, IDENTIFICATION IN THE
RP SRP COMPLEX, AND ACETYLATION AT SER-2.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7925282; DOI=10.1002/j.1460-2075.1994.tb06759.x;
RA Brown J.D., Hann B.C., Medzihradszky K.F., Niwa M., Burlingame A.L.,
RA Walter P.;
RT "Subunits of the Saccharomyces cerevisiae signal recognition particle
RT required for its functional expression.";
RL EMBO J. 13:4390-4400(1994).
RN [6]
RP FUNCTION OF THE SRP COMPLEX.
RX PubMed=8707814; DOI=10.1083/jcb.134.2.269;
RA Ng D.T., Brown J.D., Walter P.;
RT "Signal sequences specify the targeting route to the endoplasmic reticulum
RT membrane.";
RL J. Cell Biol. 134:269-278(1996).
RN [7]
RP ASSEMBLY OF THE SRP COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=11352936; DOI=10.1083/jcb.153.4.745;
RA Grosshans H., Deinert K., Hurt E.C., Simos G.;
RT "Biogenesis of the signal recognition particle (SRP) involves import of SRP
RT proteins into the nucleolus, assembly with the SRP-RNA, and Xpo1p-mediated
RT export.";
RL J. Cell Biol. 153:745-762(2001).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=17287358; DOI=10.1073/pnas.0607084104;
RA Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L.,
RA Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
RT "Analysis of phosphorylation sites on proteins from Saccharomyces
RT cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
RN [10]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Signal-recognition-particle (SRP) assembly has a crucial role
CC in targeting secretory proteins to the rough endoplasmic reticulum (ER)
CC membrane. SRP is required for the cotranslational protein translocation
CC for ER import and preferentially recognizes strongly hydrophobic signal
CC sequences. It is involved in targeting the nascent chain-ribosome (RNC)
CC complex to the ER and is proposed to participate in the arrest of
CC nascent chain elongation during membrane targeting.
CC {ECO:0000269|PubMed:8707814}.
CC -!- SUBUNIT: Fungal signal recognition particle (SRP) complex consists of a
CC 7S RNA molecule (scR1) and at least six protein subunits: SRP72, SRP68,
CC SRP54, SEC65, SRP21 and SRP14. At least SRP14, SRP21, SRP68 and SRP72
CC are proposed to get assembled together with scR1 RNA as a pre-SRP
CC complex in the nucleolus which is exported to the cytoplasm.
CC {ECO:0000269|PubMed:7925282}.
CC -!- INTERACTION:
CC P32342; P29478: SEC65; NbExp=5; IntAct=EBI-17984, EBI-16641;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus
CC {ECO:0000269|PubMed:11352936}.
CC -!- MISCELLANEOUS: Present with 3400 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; S44213; AAB23073.1; -; Genomic_DNA.
DR EMBL; Z28122; CAA81963.1; -; Genomic_DNA.
DR EMBL; AY558352; AAS56678.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09038.1; -; Genomic_DNA.
DR PIR; S25360; S25360.
DR RefSeq; NP_012800.1; NM_001179688.1.
DR AlphaFoldDB; P32342; -.
DR SMR; P32342; -.
DR BioGRID; 34013; 26.
DR ComplexPortal; CPX-609; Signal recognition particle.
DR DIP; DIP-4863N; -.
DR IntAct; P32342; 5.
DR MINT; P32342; -.
DR STRING; 4932.YKL122C; -.
DR iPTMnet; P32342; -.
DR MaxQB; P32342; -.
DR PaxDb; P32342; -.
DR PRIDE; P32342; -.
DR EnsemblFungi; YKL122C_mRNA; YKL122C; YKL122C.
DR GeneID; 853737; -.
DR KEGG; sce:YKL122C; -.
DR SGD; S000001605; SRP21.
DR VEuPathDB; FungiDB:YKL122C; -.
DR eggNOG; ENOG502S2CK; Eukaryota.
DR HOGENOM; CLU_104695_1_0_1; -.
DR InParanoid; P32342; -.
DR OMA; EPNSGKC; -.
DR BioCyc; YEAST:G3O-31905-MON; -.
DR Reactome; R-SCE-1799339; SRP-dependent cotranslational protein targeting to membrane.
DR PRO; PR:P32342; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P32342; protein.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0005786; C:signal recognition particle, endoplasmic reticulum targeting; IDA:SGD.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IDA:SGD.
DR GO; GO:0006617; P:SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition; IC:ComplexPortal.
DR InterPro; IPR039432; SRP9_dom.
DR Pfam; PF05486; SRP9-21; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Direct protein sequencing; Nucleus;
KW Reference proteome; Ribonucleoprotein; RNA-binding;
KW Signal recognition particle.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:7925282,
FT ECO:0007744|PubMed:22814378"
FT CHAIN 2..167
FT /note="Signal recognition particle subunit SRP21"
FT /id="PRO_0000135239"
FT REGION 123..167
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 138..154
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0000269|PubMed:7925282,
FT ECO:0007744|PubMed:22814378"
SQ SEQUENCE 167 AA; 18425 MW; 19C85378074C6F46 CRC64;
MSVKPIDNYI TNSVRLFEVN PSQTLFSISY KPPTQKTDTK VSFRTHNSHL SLNYKFTTNK
SKDVSRLLSA LGPRGVSITP GKIEKIAQSK KKNNKIKESS KKIKGKSIQD IVGLATLIVN
TDVEKSDPAA KKTATEPKQK ANAVQNNNGN SAASKKKKNK NKGKKKR
