ID   SRP54_ACIAM             Reviewed;         451 AA.
AC   P70722;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1999, sequence version 2.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Signal recognition particle 54 kDa protein {ECO:0000255|HAMAP-Rule:MF_00306};
DE            Short=SRP54 {ECO:0000255|HAMAP-Rule:MF_00306};
DE   Flags: Fragment;
GN   Name=srp54 {ECO:0000255|HAMAP-Rule:MF_00306}; Synonyms=ffh;
OS   Acidianus ambivalens (Desulfurolobus ambivalens).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Acidianus.
OX   NCBI_TaxID=2283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Lei 10 / DSM 3772 / JCM 9191;
RX   PubMed=9914525; DOI=10.1046/j.1432-1327.1999.00065.x;
RA   Moll R., Schaefer G., Schmidtke S.;
RT   "Domain structure, GTP-hydrolyzing activity and 7S RNA binding of Acidianus
RT   ambivalens ffh-homologous protein suggest an SRP-like complex in archaea.";
RL   Eur. J. Biochem. 259:441-448(1999).
CC   -!- FUNCTION: Involved in targeting and insertion of nascent membrane
CC       proteins into the cytoplasmic membrane. Binds to the hydrophobic signal
CC       sequence of the ribosome-nascent chain (RNC) as it emerges from the
CC       ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic
CC       membrane where it interacts with the SRP receptor FtsY.
CC       {ECO:0000255|HAMAP-Rule:MF_00306}.
CC   -!- SUBUNIT: Part of the signal recognition particle protein translocation
CC       system, which is composed of SRP and FtsY. Archaeal SRP consists of a
CC       7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and
CC       SRP19. {ECO:0000255|HAMAP-Rule:MF_00306}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00306}.
CC       Note=The SRP-RNC complex is targeted to the cytoplasmic membrane.
CC       {ECO:0000255|HAMAP-Rule:MF_00306}.
CC   -!- DOMAIN: Composed of three domains: the N-terminal N domain, which is
CC       responsible for interactions with the ribosome, the central G domain,
CC       which binds GTP, and the C-terminal M domain, which binds the RNA and
CC       the signal sequence of the RNC. {ECO:0000255|HAMAP-Rule:MF_00306}.
CC   -!- SIMILARITY: Belongs to the GTP-binding SRP family. SRP54 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00306}.
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DR   EMBL; Y08735; CAA69991.1; -; Genomic_DNA.
DR   PDB; 1J8M; X-ray; 2.00 A; F=1-292.
DR   PDB; 1J8Y; X-ray; 2.00 A; F=1-292.
DR   PDBsum; 1J8M; -.
DR   PDBsum; 1J8Y; -.
DR   AlphaFoldDB; P70722; -.
DR   SMR; P70722; -.
DR   EvolutionaryTrace; P70722; -.
DR   GO; GO:0048500; C:signal recognition particle; IEA:InterPro.
DR   GO; GO:0008312; F:7S RNA binding; IEA:InterPro.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IEA:InterPro.
DR   Gene3D; 1.10.260.30; -; 1.
DR   Gene3D; 1.20.120.140; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00306; SRP54; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR036891; Signal_recog_part_SRP54_M_sf.
DR   InterPro; IPR013822; Signal_recog_particl_SRP54_hlx.
DR   InterPro; IPR004125; Signal_recog_particle_SRP54_M.
DR   InterPro; IPR036225; SRP/SRP_N.
DR   InterPro; IPR022941; SRP54.
DR   InterPro; IPR000897; SRP54_GTPase_dom.
DR   InterPro; IPR042101; SRP54_N_sf.
DR   PANTHER; PTHR11564; PTHR11564; 1.
DR   Pfam; PF00448; SRP54; 1.
DR   Pfam; PF02881; SRP54_N; 1.
DR   Pfam; PF02978; SRP_SPB; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00962; SRP54; 1.
DR   SMART; SM00963; SRP54_N; 1.
DR   SUPFAM; SSF47364; SSF47364; 1.
DR   SUPFAM; SSF47446; SSF47446; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; GTP-binding; Nucleotide-binding;
KW   Ribonucleoprotein; RNA-binding; Signal recognition particle.
FT   CHAIN           <1..451
FT                   /note="Signal recognition particle 54 kDa protein"
FT                   /id="PRO_0000101171"
FT   BINDING         105..112
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00306"
FT   BINDING         187..191
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00306"
FT   BINDING         247..250
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00306"
FT   NON_TER         1
FT   HELIX           4..15
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   HELIX           21..38
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   HELIX           43..59
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   HELIX           68..83
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   STRAND          95..104
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   HELIX           113..124
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   STRAND          129..133
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   STRAND          137..139
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   HELIX           140..152
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   HELIX           165..178
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   STRAND          182..187
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   HELIX           197..211
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   STRAND          214..221
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   HELIX           222..228
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   HELIX           229..237
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   STRAND          242..247
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   HELIX           249..251
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   HELIX           255..263
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   TURN            264..266
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   STRAND          269..273
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   STRAND          275..277
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   STRAND          281..283
FT                   /evidence="ECO:0007829|PDB:1J8M"
FT   HELIX           286..291
FT                   /evidence="ECO:0007829|PDB:1J8M"
SQ   SEQUENCE   451 AA;  50381 MW;  EAA6F2D901748CEA CRC64;
     SKLLDNLRDA VRKFLTGSSS YDKAVEDFIK ELQKSLISAD VNVKLVFSLT NKIKERLKNE
     KPPTYIERRE WFIKIVYDEL SNLFGGDKEP KVIPDKIPYV IMLVGVQGTG KTTTAGKLAY
     FYKKKGFKVG LVGADVYRPA ALEQLQQLGQ QIGVPVYGEP GEKDAVGIAK RGVEKFLSEK
     MEIIIVDTAG RHGYGEEAAL LEEMKNIYEA IKPDEVTLVI DASIGQKAYD LASKFNQASK
     IGTIIITKMD GTAKGGGALS AVAATGATIK FIGTGEKIDE LEVFNPRRFV ARILGMGDIE
     TILEKIKEVE NYDKMQKKME EVISGKGKLT LRDVYNQLIA LRKMGPLSKL FQLLPGIGML
     GQIPEDQLKV GEEKMRKWLA IMNSMTYEEL DNPSIIDKSR MRRIALGSGT EIEDVKELIE
     HYNLMQRTIK MLKRRKKDVE KLLGQFGGES T