ID   SRP54_DICDI             Reviewed;         542 AA.
AC   Q75K18; Q552P1;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Signal recognition particle 54 kDa protein;
DE            Short=SRP54;
GN   Name=srp54; ORFNames=DDB_G0275455;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=AX2;
RX   PubMed=16926386; DOI=10.1074/mcp.m600113-mcp200;
RA   Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA   Soldati T.;
RT   "Proteomics fingerprinting of phagosome maturation and evidence for the
RT   role of a Galpha during uptake.";
RL   Mol. Cell. Proteomics 5:2228-2243(2006).
CC   -!- FUNCTION: Binds to the signal sequence of presecretory protein when
CC       they emerge from the ribosomes and transfers them to TRAM
CC       (translocating chain-associating membrane protein). {ECO:0000250}.
CC   -!- SUBUNIT: Signal recognition particle consists of a 7S RNA molecule and
CC       six protein subunits: srp72, srp68, srp54, srp19, srp14 and srp9.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- DOMAIN: Has a two domain structure: the G-domain binds GTP; the M-
CC       domain binds the 7S RNA in presence of SRP19 and also binds the signal
CC       sequence. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GTP-binding SRP family. SRP54 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000013; EAL69478.1; -; Genomic_DNA.
DR   RefSeq; XP_643640.1; XM_638548.1.
DR   AlphaFoldDB; Q75K18; -.
DR   SMR; Q75K18; -.
DR   STRING; 44689.DDB0232373; -.
DR   PaxDb; Q75K18; -.
DR   EnsemblProtists; EAL69478; EAL69478; DDB_G0275455.
DR   GeneID; 8620227; -.
DR   KEGG; ddi:DDB_G0275455; -.
DR   dictyBase; DDB_G0275455; srp54.
DR   eggNOG; KOG0780; Eukaryota.
DR   HOGENOM; CLU_009301_6_1_1; -.
DR   InParanoid; Q75K18; -.
DR   OMA; DTAGRHK; -.
DR   PhylomeDB; Q75K18; -.
DR   Reactome; R-DDI-1799339; SRP-dependent cotranslational protein targeting to membrane.
DR   PRO; PR:Q75K18; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR   GO; GO:0005786; C:signal recognition particle, endoplasmic reticulum targeting; IBA:GO_Central.
DR   GO; GO:0008312; F:7S RNA binding; IBA:GO_Central.
DR   GO; GO:0030942; F:endoplasmic reticulum signal peptide binding; IBA:GO_Central.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0006616; P:SRP-dependent cotranslational protein targeting to membrane, translocation; IBA:GO_Central.
DR   Gene3D; 1.10.260.30; -; 1.
DR   Gene3D; 1.20.120.140; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00306; SRP54; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR036891; Signal_recog_part_SRP54_M_sf.
DR   InterPro; IPR013822; Signal_recog_particl_SRP54_hlx.
DR   InterPro; IPR004125; Signal_recog_particle_SRP54_M.
DR   InterPro; IPR036225; SRP/SRP_N.
DR   InterPro; IPR022941; SRP54.
DR   InterPro; IPR006325; SRP54_euk.
DR   InterPro; IPR000897; SRP54_GTPase_dom.
DR   InterPro; IPR042101; SRP54_N_sf.
DR   PANTHER; PTHR11564; PTHR11564; 1.
DR   Pfam; PF00448; SRP54; 1.
DR   Pfam; PF02881; SRP54_N; 1.
DR   Pfam; PF02978; SRP_SPB; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00962; SRP54; 1.
DR   SMART; SM00963; SRP54_N; 1.
DR   SUPFAM; SSF47364; SSF47364; 1.
DR   SUPFAM; SSF47446; SSF47446; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01425; SRP54_euk; 1.
DR   PROSITE; PS00300; SRP54; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; GTP-binding; Nucleotide-binding; Reference proteome;
KW   Ribonucleoprotein; RNA-binding; Signal recognition particle.
FT   CHAIN           1..542
FT                   /note="Signal recognition particle 54 kDa protein"
FT                   /id="PRO_0000327678"
FT   REGION          1..295
FT                   /note="G-domain"
FT   REGION          296..542
FT                   /note="M-domain"
FT   BINDING         108..115
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         190..194
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         248..251
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   542 AA;  57610 MW;  F5AE74963AD00D20 CRC64;
     MVLADLGNQL SSALRSLNET TIVNEDTINQ LLKEVGNALS KSDVSMSLII QMRKNIKDKI
     KLDQMAAGLN KRKIIKQVVF DELIRLLDPG VPLWKPTKGK SNIVMFVGLQ GAGKTTSVTK
     LAYFYKKKGF STAIVCADTF RAGAFDQVRH NAAKAKIHYY GSETEKDPVV VARTGVDIFK
     KDGTEIIIVD TSGRHKQDSE LFEEMKQIET AVKPDNCIFV MDSSIGQAAY EQATAFRSSV
     KVGSIIITKM DGNSMGGGAI SAVAATNTPI IFIGTGEHLT DLELFDPSTF VSKLLGYGDM
     KGMLEKIKEV IPEDSTSLKE IAQGKFTLRS MQQQFQQIMQ LGPIDKLVQM IPGMNQLPQL
     QGNEGGLKLK AYINILDSLS EKELDGKKPI TQKRIITIAQ GSGRHPNEVV ELLEQHKTFE
     KLIGKGGPGG GLGSLMAGKG GPKNMEQAMK QMNANGGMQG LMNSLKGMGG MGDLAKMFGG
     GGGGGGGMPS MGDLAKMMGG MGGGGRGGGG MPNMGDLAKM MGGMGGGAGK GGQNGFPNLD
     LD