ID   SRP54_ENTHI             Reviewed;         487 AA.
AC   O15821;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Signal recognition particle 54 kDa protein;
DE            Short=SRP54;
OS   Entamoeba histolytica.
OC   Eukaryota; Amoebozoa; Evosea; Archamoebae; Mastigamoebida; Entamoebidae;
OC   Entamoeba.
OX   NCBI_TaxID=5759;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 30459 / HM-1:IMSS;
RX   PubMed=9274882; DOI=10.1016/s0166-6851(97)00096-0;
RA   Ramos M.A., Mercado G.C., Salgado L.M., Sanchez-Lopez R., Stock R.P.,
RA   Lizardi P.M., Alagon A.;
RT   "Entamoeba histolytica contains a gene encoding a homologue to the 54 kDa
RT   subunit of the signal recognition particle.";
RL   Mol. Biochem. Parasitol. 88:225-235(1997).
CC   -!- FUNCTION: Binds to the signal sequence of presecretory protein when
CC       they emerge from the ribosomes and transfers them to TRAM
CC       (translocating chain-associating membrane protein).
CC   -!- SUBUNIT: Signal recognition particle consists of a 7S RNA molecule of
CC       300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19,
CC       SRP14 and SRP9. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- DOMAIN: Has a two domain structure: the G-domain binds GTP; the M-
CC       domain binds the 7S RNA in presence of SRP19 and also binds the signal
CC       sequence.
CC   -!- SIMILARITY: Belongs to the GTP-binding SRP family. SRP54 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AF386797; AAL50553.1; -; Genomic_DNA.
DR   AlphaFoldDB; O15821; -.
DR   SMR; O15821; -.
DR   STRING; 5759.rna_EHI_004750-1; -.
DR   VEuPathDB; AmoebaDB:EHI5A_201350; -.
DR   VEuPathDB; AmoebaDB:EHI7A_194930; -.
DR   VEuPathDB; AmoebaDB:EHI8A_228890; -.
DR   VEuPathDB; AmoebaDB:EHI_004750; -.
DR   VEuPathDB; AmoebaDB:KM1_291640; -.
DR   eggNOG; KOG0780; Eukaryota.
DR   GO; GO:0005786; C:signal recognition particle, endoplasmic reticulum targeting; IEA:UniProtKB-KW.
DR   GO; GO:0008312; F:7S RNA binding; IEA:InterPro.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IEA:InterPro.
DR   Gene3D; 1.10.260.30; -; 1.
DR   Gene3D; 1.20.120.140; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00306; SRP54; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR036891; Signal_recog_part_SRP54_M_sf.
DR   InterPro; IPR013822; Signal_recog_particl_SRP54_hlx.
DR   InterPro; IPR004125; Signal_recog_particle_SRP54_M.
DR   InterPro; IPR036225; SRP/SRP_N.
DR   InterPro; IPR022941; SRP54.
DR   InterPro; IPR006325; SRP54_euk.
DR   InterPro; IPR000897; SRP54_GTPase_dom.
DR   InterPro; IPR042101; SRP54_N_sf.
DR   PANTHER; PTHR11564; PTHR11564; 1.
DR   Pfam; PF00448; SRP54; 1.
DR   Pfam; PF02881; SRP54_N; 1.
DR   Pfam; PF02978; SRP_SPB; 1.
DR   SMART; SM00962; SRP54; 1.
DR   SMART; SM00963; SRP54_N; 1.
DR   SUPFAM; SSF47364; SSF47364; 1.
DR   SUPFAM; SSF47446; SSF47446; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01425; SRP54_euk; 1.
DR   PROSITE; PS00300; SRP54; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; GTP-binding; Nucleotide-binding; Ribonucleoprotein; RNA-binding;
KW   Signal recognition particle.
FT   CHAIN           1..487
FT                   /note="Signal recognition particle 54 kDa protein"
FT                   /id="PRO_0000101197"
FT   REGION          1..295
FT                   /note="G-domain"
FT   REGION          296..487
FT                   /note="M-domain"
FT   BINDING         108..115
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         190..194
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         248..251
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   487 AA;  53857 MW;  5377F3209F5A8851 CRC64;
     MVLSQLGSSL VTALRKMTSS TVVDEEVINT LLKEIETSLL GEDVNPIFIR QMVNNIKKKI
     NSEDIPDGID KRKLIKDSVF EELINLVDPK TEAFKPKKGK TCVLMMVGLQ GAGKTTTITK
     LALYYKNRGY KPAVVGADTF RAGAYEQLQM NAKRAGVPFF GIKEESDPVK VASEGVRTFR
     KEKNDIILVD TSGRHKQDKE LFKEMQSVRD AIKPDSIIFV MDGAIGQAAF GQAKAFKDAV
     EVGSVIITKL DGHSNGGGAL SAVAATKSPI IFIGTGEKVN EIEEFDAESF VRQLLGMGDL
     KGIAKLAKDF AENAEYKTMV KHLQEGTLTV RDWKEQLSNL QKMGQLGNIM QMIGLNHPMF
     QGGNIEKKFK VFMVILDSMT DRELDGSAKT MLNDESRIRR LARGSGRDIR EVNELFEQIK
     LFQQCIDRLP KAMRAQLGNC NAQPNEAAMM QQMQRMLPKG VNQAQLQQLM KQMNAAGLGG
     TGKKGKK