ID   SRP54_MYCMY             Reviewed;         447 AA.
AC   Q01442;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Signal recognition particle protein {ECO:0000255|HAMAP-Rule:MF_00306};
DE   AltName: Full=Fifty-four homolog {ECO:0000255|HAMAP-Rule:MF_00306};
GN   Name=ffh {ECO:0000255|HAMAP-Rule:MF_00306}; Synonyms=srpM54;
OS   Mycoplasma mycoides.
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=2102;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INTERACTION WITH RNA.
RX   PubMed=1280809; DOI=10.1093/nar/20.21.5763;
RA   Samuelsson T.B.;
RT   "A Mycoplasma protein homologous to mammalian SRP54 recognizes a highly
RT   conserved domain of SRP RNA.";
RL   Nucleic Acids Res. 20:5763-5770(1992).
CC   -!- FUNCTION: Involved in targeting and insertion of nascent membrane
CC       proteins into the cytoplasmic membrane. Binds to the hydrophobic signal
CC       sequence of the ribosome-nascent chain (RNC) as it emerges from the
CC       ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic
CC       membrane where it interacts with the SRP receptor FtsY.
CC       {ECO:0000255|HAMAP-Rule:MF_00306}.
CC   -!- SUBUNIT: Part of the signal recognition particle protein translocation
CC       system, which is composed of SRP and FtsY (By similarity). Interacts
CC       with RNA. {ECO:0000255|HAMAP-Rule:MF_00306,
CC       ECO:0000269|PubMed:1280809}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00306}.
CC       Note=The SRP-RNC complex is targeted to the cytoplasmic membrane.
CC       {ECO:0000255|HAMAP-Rule:MF_00306}.
CC   -!- DOMAIN: Composed of three domains: the N-terminal N domain, which is
CC       responsible for interactions with the ribosome, the central G domain,
CC       which binds GTP, and the C-terminal M domain, which binds the RNA and
CC       the signal sequence of the RNC. {ECO:0000255|HAMAP-Rule:MF_00306}.
CC   -!- SIMILARITY: Belongs to the GTP-binding SRP family. SRP54 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00306}.
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DR   EMBL; M91593; AAA25441.1; -; Genomic_DNA.
DR   PIR; S35481; S35481.
DR   AlphaFoldDB; Q01442; -.
DR   SMR; Q01442; -.
DR   PRIDE; Q01442; -.
DR   GO; GO:0048500; C:signal recognition particle; IEA:UniProtKB-UniRule.
DR   GO; GO:0008312; F:7S RNA binding; IEA:InterPro.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IEA:InterPro.
DR   Gene3D; 1.10.260.30; -; 1.
DR   Gene3D; 1.20.120.140; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00306; SRP54; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR036891; Signal_recog_part_SRP54_M_sf.
DR   InterPro; IPR013822; Signal_recog_particl_SRP54_hlx.
DR   InterPro; IPR004125; Signal_recog_particle_SRP54_M.
DR   InterPro; IPR004780; SRP.
DR   InterPro; IPR022941; SRP54.
DR   InterPro; IPR000897; SRP54_GTPase_dom.
DR   InterPro; IPR042101; SRP54_N_sf.
DR   PANTHER; PTHR11564; PTHR11564; 1.
DR   PANTHER; PTHR11564:SF7; PTHR11564:SF7; 1.
DR   Pfam; PF00448; SRP54; 1.
DR   Pfam; PF02881; SRP54_N; 1.
DR   Pfam; PF02978; SRP_SPB; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00962; SRP54; 1.
DR   SMART; SM00963; SRP54_N; 1.
DR   SUPFAM; SSF47446; SSF47446; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00959; ffh; 1.
DR   PROSITE; PS00300; SRP54; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; GTP-binding; Nucleotide-binding; Ribonucleoprotein; RNA-binding;
KW   Signal recognition particle.
FT   CHAIN           1..447
FT                   /note="Signal recognition particle protein"
FT                   /id="PRO_0000101160"
FT   BINDING         108..115
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00306"
FT   BINDING         190..194
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00306"
FT   BINDING         248..251
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00306"
SQ   SEQUENCE   447 AA;  50100 MW;  24A71128D4041D43 CRC64;
     MGFGDFLSKR MQKSIEKNMK NSTLNEENIK ETLKEIRLSL LEADVNIEAA KEIINNVKQK
     ALGGYISEGA SAHQQMIKIV HEELVNILGK ENAPLDINKK PSVVMMVGLQ GSGKTTTANK
     LAYLLNKKNK KKVLLVGLDI YRPGAIEQLV QLGQKTNTQV FEKGKQDPVK TAEQALEYAK
     ENNFDVVILD TAGRLQVDQV LMKELDNLKK KTSPNEILLV VDGMSGQEII NVTNEFNDKL
     KLSGVVVTKL DGDARGGATL SISYLTKLPI KFIGEGEGYN ALAAFYPKRM ADRLMGMGDI
     ETLFERAVEN IDERSIQKTM NRMFLGQFDL EDLRNQLAQI AKMGSLNKLM KMLPINKVSE
     SQIQEAQRKL AVFSILMDSM TLKERRDPRV LKAISRKNRI IKGSGRSEKE FNELINSFEK
     GKKQVLEITK MIKSGRMPNL SKGGFKF