ID   SRP54_MYCPN             Reviewed;         450 AA.
AC   P75054;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Signal recognition particle protein {ECO:0000255|HAMAP-Rule:MF_00306};
DE   AltName: Full=Fifty-four homolog {ECO:0000255|HAMAP-Rule:MF_00306};
GN   Name=ffh {ECO:0000255|HAMAP-Rule:MF_00306}; OrderedLocusNames=MPN_061;
GN   ORFNames=MP093;
OS   Mycoplasma pneumoniae (strain ATCC 29342 / M129) (Mycoplasmoides
OS   pneumoniae).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=272634;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29342 / M129;
RX   PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA   Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.;
RT   "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT   pneumoniae.";
RL   Nucleic Acids Res. 24:4420-4449(1996).
CC   -!- FUNCTION: Involved in targeting and insertion of nascent membrane
CC       proteins into the cytoplasmic membrane. Binds to the hydrophobic signal
CC       sequence of the ribosome-nascent chain (RNC) as it emerges from the
CC       ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic
CC       membrane where it interacts with the SRP receptor FtsY.
CC       {ECO:0000255|HAMAP-Rule:MF_00306}.
CC   -!- SUBUNIT: Part of the signal recognition particle protein translocation
CC       system, which is composed of SRP and FtsY. {ECO:0000255|HAMAP-
CC       Rule:MF_00306}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00306}.
CC       Note=The SRP-RNC complex is targeted to the cytoplasmic membrane.
CC       {ECO:0000255|HAMAP-Rule:MF_00306}.
CC   -!- DOMAIN: Composed of three domains: the N-terminal N domain, which is
CC       responsible for interactions with the ribosome, the central G domain,
CC       which binds GTP, and the C-terminal M domain, which binds the RNA and
CC       the signal sequence of the RNC. {ECO:0000255|HAMAP-Rule:MF_00306}.
CC   -!- SIMILARITY: Belongs to the GTP-binding SRP family. SRP54 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00306}.
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DR   EMBL; U00089; AAB95741.1; -; Genomic_DNA.
DR   PIR; S73419; S73419.
DR   RefSeq; NP_109749.1; NC_000912.1.
DR   RefSeq; WP_010874418.1; NC_000912.1.
DR   AlphaFoldDB; P75054; -.
DR   SMR; P75054; -.
DR   IntAct; P75054; 4.
DR   STRING; 272634.MPN_061; -.
DR   EnsemblBacteria; AAB95741; AAB95741; MPN_061.
DR   KEGG; mpn:MPN_061; -.
DR   PATRIC; fig|272634.6.peg.62; -.
DR   HOGENOM; CLU_009301_6_0_14; -.
DR   OMA; DTAGRHK; -.
DR   BioCyc; MPNE272634:G1GJ3-97-MON; -.
DR   Proteomes; UP000000808; Chromosome.
DR   GO; GO:0048500; C:signal recognition particle; IEA:UniProtKB-UniRule.
DR   GO; GO:0008312; F:7S RNA binding; IEA:InterPro.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IEA:InterPro.
DR   Gene3D; 1.10.260.30; -; 1.
DR   Gene3D; 1.20.120.140; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   HAMAP; MF_00306; SRP54; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR036891; Signal_recog_part_SRP54_M_sf.
DR   InterPro; IPR013822; Signal_recog_particl_SRP54_hlx.
DR   InterPro; IPR004125; Signal_recog_particle_SRP54_M.
DR   InterPro; IPR004780; SRP.
DR   InterPro; IPR036225; SRP/SRP_N.
DR   InterPro; IPR022941; SRP54.
DR   InterPro; IPR000897; SRP54_GTPase_dom.
DR   InterPro; IPR042101; SRP54_N_sf.
DR   PANTHER; PTHR11564; PTHR11564; 1.
DR   PANTHER; PTHR11564:SF7; PTHR11564:SF7; 1.
DR   Pfam; PF00448; SRP54; 1.
DR   Pfam; PF02881; SRP54_N; 1.
DR   Pfam; PF02978; SRP_SPB; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00962; SRP54; 1.
DR   SMART; SM00963; SRP54_N; 1.
DR   SUPFAM; SSF47364; SSF47364; 1.
DR   SUPFAM; SSF47446; SSF47446; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00959; ffh; 1.
DR   PROSITE; PS00300; SRP54; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; GTP-binding; Nucleotide-binding; Reference proteome;
KW   Ribonucleoprotein; RNA-binding; Signal recognition particle.
FT   CHAIN           1..450
FT                   /note="Signal recognition particle protein"
FT                   /id="PRO_0000101161"
FT   BINDING         106..113
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00306"
FT   BINDING         188..192
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00306"
FT   BINDING         246..249
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00306"
SQ   SEQUENCE   450 AA;  50141 MW;  7EFE42ECEA4FE679 CRC64;
     MFKSMISSIV MRSMQKKINA QTISETDVQA VLKEIRIALL DADVNLLVVK NFIKAIREQT
     VGQTVEPGQD LQKWLLKVIK QELINILSQP NQEITSKRPL KVMVVGLQGS GKTTTCGKLA
     VWLKKQFQQK AMLVALDIYR PAAIDQLATL AEQTESVFFA KGTQAPDQTT KEAVKTFKES
     GCQAIICDTA GRLQTNQELM DELVAIKNEL HPDEILMVVD GLSGQEIINV AKEFHNRLKL
     TGFIITKLDS DARAGAALSL TSLLEVPIKL MGTSEKLTGL EQFHPERIAS RILGLGDVMT
     LVEKAEEVFD KQSLTKTVSK MFLGKMDLED LLLYMEQMNQ MGSVSSIVKM LPGNLTVSDD
     HVESIEAKVK LWKVLINSMT REERRHPKLI NRDPSRKQRI IKGSGRKMDE LNKLMKEWSK
     LQTKTAEMGR MLKGGKNPFS GFGGLGGLGF