ID   SRP68_DICDI             Reviewed;         614 AA.
AC   Q1ZXE8;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Signal recognition particle subunit SRP68;
DE   AltName: Full=Signal recognition particle 68 kDa protein homolog;
GN   Name=srp68; ORFNames=DDB_G0285821;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Component of the signal recognition particle (SRP) complex, a
CC       ribonucleoprotein complex that mediates the cotranslational targeting
CC       of secretory and membrane proteins to the endoplasmic reticulum (ER)
CC       (By similarity). The SRP complex interacts with the signal sequence in
CC       nascent secretory and membrane proteins and directs them to the
CC       membrane of the ER (By similarity). The SRP complex targets the
CC       ribosome-nascent chain complex to the SRP receptor (SR), which is
CC       anchored in the ER, where SR compaction and GTPase rearrangement drive
CC       cotranslational protein translocation into the ER (By similarity).
CC       Binds the 7SL RNA, srp72 binds to this complex subsequently (By
CC       similarity). The SRP complex possibly participates in the elongation
CC       arrest function (By similarity). {ECO:0000250|UniProtKB:P38687,
CC       ECO:0000250|UniProtKB:Q9UHB9}.
CC   -!- SUBUNIT: Heterodimer with srp72 (By similarity). Srp68-srp72
CC       heterodimer formation is stabilized by the presence of 7SL RNA (By
CC       similarity). Component of a signal recognition particle (SRP) complex
CC       that consists of a 7SL RNA molecule and six protein subunits: srp72,
CC       srp68, srp54, srp19, srp14 and srp9 (By similarity). Within the SRP
CC       complex, interacts (via C-terminus) with srp72 (via N-terminus) (By
CC       similarity). {ECO:0000250|UniProtKB:Q00004,
CC       ECO:0000250|UniProtKB:Q9UHB9}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9UHB9}.
CC       Nucleus, nucleolus {ECO:0000250|UniProtKB:Q9UHB9}. Endoplasmic
CC       reticulum {ECO:0000250|UniProtKB:Q9UHB9}.
CC   -!- DOMAIN: The N-terminus is required for RNA-binding.
CC       {ECO:0000250|UniProtKB:Q9UHB9}.
CC   -!- SIMILARITY: Belongs to the SRP68 family. {ECO:0000305}.
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DR   EMBL; AAFI02000080; EAS66853.1; -; Genomic_DNA.
DR   RefSeq; XP_001134536.1; XM_001134536.1.
DR   AlphaFoldDB; Q1ZXE8; -.
DR   SMR; Q1ZXE8; -.
DR   STRING; 44689.DDB0232383; -.
DR   PaxDb; Q1ZXE8; -.
DR   EnsemblProtists; EAS66853; EAS66853; DDB_G0285821.
DR   GeneID; 8625304; -.
DR   KEGG; ddi:DDB_G0285821; -.
DR   dictyBase; DDB_G0285821; srp68.
DR   eggNOG; KOG2460; Eukaryota.
DR   HOGENOM; CLU_018649_0_1_1; -.
DR   InParanoid; Q1ZXE8; -.
DR   OMA; DERFIHI; -.
DR   PhylomeDB; Q1ZXE8; -.
DR   Reactome; R-DDI-1799339; SRP-dependent cotranslational protein targeting to membrane.
DR   PRO; PR:Q1ZXE8; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005786; C:signal recognition particle, endoplasmic reticulum targeting; ISS:dictyBase.
DR   GO; GO:0008312; F:7S RNA binding; IEA:InterPro.
DR   GO; GO:0030942; F:endoplasmic reticulum signal peptide binding; IEA:InterPro.
DR   GO; GO:0005047; F:signal recognition particle binding; IBA:GO_Central.
DR   GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IEA:InterPro.
DR   CDD; cd15481; SRP68-RBD; 1.
DR   Gene3D; 1.10.3450.40; -; 1.
DR   InterPro; IPR026258; SRP68.
DR   InterPro; IPR034652; SRP68-RBD.
DR   InterPro; IPR038253; SRP68_N_sf.
DR   PANTHER; PTHR12860; PTHR12860; 1.
DR   Pfam; PF16969; SRP68; 1.
DR   PIRSF; PIRSF038995; SRP68; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Endoplasmic reticulum; Nucleus; Reference proteome;
KW   Ribonucleoprotein; RNA-binding; Signal recognition particle.
FT   CHAIN           1..614
FT                   /note="Signal recognition particle subunit SRP68"
FT                   /id="PRO_0000327627"
SQ   SEQUENCE   614 AA;  70904 MW;  B232C1407F40707F CRC64;
     MATNISTSPK EDVPKEKFHL DILNFSQTSQ IQFGLRLQDY KKYRQYCSKR IQRLRSQLRK
     QYGKKNYVNK IVLNGGETEK QINDVRYLQI SLLKTERAWS YAMDLKAQFE KDNDSRIGFH
     MNRRFGKASR NSTQLYELCK LVADQYTIIE AHAYSSWMAS SLSLTKQDFK KALEEINISK
     TIYEKLSEQG DHSQKELYQK RIEETEPIIR FCLYNLKNEK NTSNKVSQNT LTIIEQSLEE
     LKNQENKKKS STSSTTTTTA TTTIEWKSKS IKVDEKLDEK LKIFNEFKKE NENKLPKNIN
     ASIDTTSNIS PIFNIYDKLV YNLINCETIV KNDLSTLVRV NLKNKSVKSE IEEQSTRILL
     AYIMYHKMKY LYERNSILIT MMQRVLDGEK VDESAIKKKV KKVTWNDLVR LSTHQVKVYT
     IMSESRDTDS NKEIARDQDA QLILLKSIRL YYIAVCLAKS LKFSETMAVL DRVLTNIQNV
     RKTNTKNQQI LNDTNELESN IKKQRSQIHA NSFIQQLSEN QDLKNQMSSL SIGGITTASG
     KSNDLISGLD SFDTSFLTEK KLVQLPPQLQ PVQAKPIFFD LAFNSVTFPS LDQRKKGSST
     GTPSKGLFGF WGRG