ID   SRPA_PSEPU              Reviewed;         382 AA.
AC   O31099;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Solvent efflux pump periplasmic linker SrpA;
DE   Flags: Precursor;
GN   Name=srpA;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND EFFLUX PUMP SUBSTRATES.
RC   STRAIN=ATCC 700801 / S12;
RX   PubMed=9417051; DOI=10.1074/jbc.273.1.85;
RA   Kieboom J., Dennis J.J., de Bont J.A.M., Zylstra G.J.;
RT   "Identification and molecular characterization of an efflux pump involved
RT   in Pseudomonas putida S12 solvent tolerance.";
RL   J. Biol. Chem. 273:85-91(1998).
RN   [2]
RP   INDUCTION.
RC   STRAIN=ATCC 700801 / S12;
RX   PubMed=9852029; DOI=10.1128/jb.180.24.6769-6772.1998;
RA   Kieboom J., Dennis J.J., Zylstra G.J., de Bont J.A.M.;
RT   "Active efflux of organic solvents by Pseudomonas putida S12 is induced by
RT   solvents.";
RL   J. Bacteriol. 180:6769-6772(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 700801 / S12;
RA   Kieboom J., Dennis J.J., Zylstra G.J., de Bont J.A.M.;
RL   Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The periplasmic linker protein component of an organic
CC       solvent efflux pump. Involved in export of a number of low log POW
CC       compounds including hexane (log POW 3.5), toluene (log POW 2.5) and
CC       dimethylphthalate (log POW 2.3). The solvent resistance phenotype has
CC       been postulated to depend on the operon expression level.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- INDUCTION: Low constitutive expression; the srpABC operon is further
CC       induced up to 17-fold by organic solvents (e.g. toluene and aliphatic
CC       solvents and alcohols) but not by antibiotics, heavy metals, pH,
CC       temperature, high NaCl or 60 mM acetic acid.
CC       {ECO:0000269|PubMed:9852029}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF029405; AAD12178.1; -; Genomic_DNA.
DR   AlphaFoldDB; O31099; -.
DR   SMR; O31099; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR043602; CusB_dom_1.
DR   InterPro; IPR032317; HlyD_D23.
DR   InterPro; IPR006143; RND_pump_MFP.
DR   Pfam; PF00529; CusB_dom_1; 1.
DR   Pfam; PF16576; HlyD_D23; 1.
DR   TIGRFAMs; TIGR01730; RND_mfp; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   2: Evidence at transcript level;
KW   Cell inner membrane; Cell membrane; Coiled coil; Lipoprotein; Membrane;
KW   Palmitate; Signal; Transport.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           24..382
FT                   /note="Solvent efflux pump periplasmic linker SrpA"
FT                   /id="PRO_0000018716"
FT   COILED          98..127
FT                   /evidence="ECO:0000255"
FT   LIPID           24
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           24
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   382 AA;  41429 MW;  8B7AB0A2DC4C440A CRC64;
     MRQIRSPRAL RVIPLTALML ISGCGEKEQV SSATPPPDVG VYTVRAQALT LTTDLPGRTS
     AFRVAEVRPQ VSGILQKRSF VEGAEVKLGQ QLYQIDPRTY EAQLRRAEAN RTSAQNLARR
     YETLLKTKAV SKQQYDDALA AWKQAEADYQ VARIDVQYTR VLSPISGRIG RSTVTEGALV
     TNGQAQSLAT VTQLDPIYVD VTQPITKLLG LQKALESGRL QKTGENQAEV SLTLDDGSAY
     PLPGTLKFSE VSVDPTTGSV TLRAEFPNPN RKLLPGMFVH ALLKEGVQNA AILVPQQAIS
     RDTRGVPSVW VVKADNTVES REIQTLRTVG NAWLISNGVT EGERIITEGV QRVRSGIAVN
     AVEAKNVNLV DGFAATTEAS AN