ID   SRPB_PSEPU              Reviewed;        1049 AA.
AC   O31100;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Solvent-resistant pump membrane transporter SrpB;
GN   Name=srpB;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND EFFLUX PUMP SUBSTRATES.
RC   STRAIN=ATCC 700801 / S12;
RX   PubMed=9417051; DOI=10.1074/jbc.273.1.85;
RA   Kieboom J., Dennis J.J., de Bont J.A.M., Zylstra G.J.;
RT   "Identification and molecular characterization of an efflux pump involved
RT   in Pseudomonas putida S12 solvent tolerance.";
RL   J. Biol. Chem. 273:85-91(1998).
RN   [2]
RP   INDUCTION.
RC   STRAIN=ATCC 700801 / S12;
RX   PubMed=9852029; DOI=10.1128/jb.180.24.6769-6772.1998;
RA   Kieboom J., Dennis J.J., Zylstra G.J., de Bont J.A.M.;
RT   "Active efflux of organic solvents by Pseudomonas putida S12 is induced by
RT   solvents.";
RL   J. Bacteriol. 180:6769-6772(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 700801 / S12;
RA   Kieboom J., Dennis J.J., Zylstra G.J., de Bont J.A.M.;
RL   Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The inner membrane transporter component of an organic
CC       solvent efflux pump. Involved in export of a number of low log POW
CC       compounds including hexane (log POW 3.5), toluene (log POW 2.5) and
CC       dimethylphthalate (log POW 2.3). The solvent resistance phenotype has
CC       been postulated to depend on the operon expression level.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- INDUCTION: Low constitutive expression; the srpABC operon is further
CC       induced up to 17-fold by organic solvents (e.g. toluene and aliphatic
CC       solvents and alcohols) but not by antibiotics, heavy metals, pH,
CC       temperature, high NaCl or 60 mM acetic acid.
CC       {ECO:0000269|PubMed:9852029}.
CC   -!- SIMILARITY: Belongs to the resistance-nodulation-cell division (RND)
CC       (TC 2.A.6) family. {ECO:0000305}.
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DR   EMBL; AF029405; AAD12176.1; -; Genomic_DNA.
DR   RefSeq; WP_014003970.1; NZ_SPUU01000035.1.
DR   AlphaFoldDB; O31100; -.
DR   SMR; O31100; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0042908; P:xenobiotic transport; IEA:InterPro.
DR   Gene3D; 3.30.2090.10; -; 2.
DR   InterPro; IPR027463; AcrB_DN_DC_subdom.
DR   InterPro; IPR001036; Acrflvin-R.
DR   InterPro; IPR004764; HAE1.
DR   PANTHER; PTHR32063; PTHR32063; 1.
DR   Pfam; PF00873; ACR_tran; 1.
DR   PRINTS; PR00702; ACRIFLAVINRP.
DR   SUPFAM; SSF82714; SSF82714; 2.
DR   TIGRFAMs; TIGR00915; 2A0602; 1.
PE   2: Evidence at transcript level;
KW   Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..1049
FT                   /note="Solvent-resistant pump membrane transporter SrpB"
FT                   /id="PRO_0000161855"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        339..359
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        366..386
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        392..412
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        440..460
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        470..490
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        542..562
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        871..891
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        895..915
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        927..947
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        973..993
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1008..1028
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1049 AA;  114110 MW;  A5A0782BE2BBCD81 CRC64;
     MSRFFIDRPI FAWVLAIVAM LAGALSLAKM PISQYPNIAA PAVSIQVSYP GASAQTVQDT
     VVQVIEQQLS GLDGFRYMSA ESASDGSMTI IVTFEQGTDP DIAQVQVQNK LQLATPRLPE
     EVQRQGLRVV KYQMNFFLVM SLVDRSGKLD NFDLGNLIAS QLQDPISRIP GVGDFQLFGS
     PYAMRIWLDP GKLNSYQLTP TDVASAIREQ NVQVSSGQLG GLPTRSGVQL NATVLGKTRM
     TTPSQFDEIL VKVNPDGSQV RVKDVGRAEL GADSFAISAQ YKDSPTASLA LRLSTGGNLL
     ETVDAVKKLM EQQKAYLPDG VEVIYPYDTT PVVEASIESV VHTIFEAVVL VFLVMYLFLQ
     SFRATLIPTL AVPVVLLATF ALLPYFGLNI NVLTMYAMVL AIGLLVDDAI VVVENVERLM
     HDEGLSPLEA TRKSMDQISG ALVGIGMVLS AVFVPMAFFG GSAGIIYQQF AITIVVCMGL
     SILVALVFTP ALCVTILKAP EGNSHHERKG FFGWFNRIFD RGTRRFERGV GAMLKGRGRY
     LLAFLLITGG TGYLFTQIPK AFLPNEDQGL MMIEVRTPAN ASAERTEGVL QEVRDYLAND
     EGALVEHFMT VNGFNFAGRG QNSGLVLITF KDWKERHGAG QDVFSIAQRA NQHFAKIKDA
     SVMAFVPPAI LEMGNAMGFN LYLQDNLGLG HEALMAARNQ FLQLASQNPK LQAVRPNGKD
     DEPQFQVNID DEKARALQVS IASINETMSA AWGSMYVNDF IDRGRVKRVY VQGEDISRIS
     PEDFDKWYVR NSLGQMVPFS AFATGEWVNG SPKLERYGGI SSLNILGEPA PGYSTGDAMI
     AIAEIMQQLP AGIGLSYTGL SYEEIQTGDQ APLLYALTVL IVFLCLAALY ESWSVPVSVI
     MVVPLGILGA VLATLWRDLT ADVYFQVGLM TTVGLSAKNA ILIVEFAKEL YEKEGYPIVK
     AAIEAAKLRL RPILMTSLAF TFGVLPMAIA SGAGAGSQHS IATGVVGGMI TATVLAVFFV
     PLFYVVVVKL FEGLMKRKPN AVKEVTHEV