SRPB_YEAST
ID SRPB_YEAST Reviewed; 244 AA.
AC P36057; D6VX44;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 187.
DE RecName: Full=Signal recognition particle receptor subunit beta;
DE Short=SR-beta;
GN Name=SRP102; OrderedLocusNames=YKL154W; ORFNames=YKL609;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091859; DOI=10.1002/yea.320100005;
RA Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F.;
RT "DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci
RT of chromosome XI of Saccharomyces cerevisiae.";
RL Yeast 10:S35-S40(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 27-244 IN COMPLEX WITH GTP AND
RP SRP101, AND SUBUNIT.
RX PubMed=12654246; DOI=10.1016/s0092-8674(03)00161-2;
RA Schwartz T., Blobel G.;
RT "Structural basis for the function of the beta subunit of the eukaryotic
RT signal recognition particle receptor.";
RL Cell 112:793-803(2003).
CC -!- FUNCTION: Component of the signal recognition particle (SRP) complex
CC receptor (SR) (By similarity). Ensures, in conjunction with the SRP
CC complex, the correct targeting of the nascent secretory proteins to the
CC endoplasmic reticulum membrane system (By similarity). May mediate the
CC membrane association of SR (By similarity).
CC {ECO:0000250|UniProtKB:P47758}.
CC -!- SUBUNIT: Heterodimer of an alpha and a beta chain.
CC {ECO:0000269|PubMed:12654246}.
CC -!- INTERACTION:
CC P36057; P32916: SRP101; NbExp=5; IntAct=EBI-18091, EBI-18098;
CC P36057; P36057: SRP102; NbExp=2; IntAct=EBI-18091, EBI-18091;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:O13950}; Single-pass membrane protein
CC {ECO:0000255}.
CC -!- MISCELLANEOUS: Present with 8250 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the SRP receptor beta subunit family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA81499.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; Z26877; CAA81499.1; ALT_INIT; Genomic_DNA.
DR EMBL; Z28154; CAA81995.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09010.1; -; Genomic_DNA.
DR PIR; S37984; S37984.
DR RefSeq; NP_012768.1; NM_001179720.1.
DR PDB; 1NRJ; X-ray; 1.70 A; B=31-244.
DR PDB; 2GED; X-ray; 2.20 A; A/B=36-183.
DR PDBsum; 1NRJ; -.
DR PDBsum; 2GED; -.
DR AlphaFoldDB; P36057; -.
DR SMR; P36057; -.
DR BioGRID; 33983; 426.
DR ComplexPortal; CPX-780; Signal recognition particle receptor complex.
DR DIP; DIP-4753N; -.
DR IntAct; P36057; 15.
DR MINT; P36057; -.
DR STRING; 4932.YKL154W; -.
DR TCDB; 3.A.5.8.1; the general secretory pathway (sec) family.
DR iPTMnet; P36057; -.
DR MaxQB; P36057; -.
DR PaxDb; P36057; -.
DR PRIDE; P36057; -.
DR EnsemblFungi; YKL154W_mRNA; YKL154W; YKL154W.
DR GeneID; 853702; -.
DR KEGG; sce:YKL154W; -.
DR SGD; S000001637; SRP102.
DR VEuPathDB; FungiDB:YKL154W; -.
DR eggNOG; KOG0090; Eukaryota.
DR GeneTree; ENSGT00940000167928; -.
DR HOGENOM; CLU_091084_1_0_1; -.
DR InParanoid; P36057; -.
DR OMA; NKQDVAM; -.
DR BioCyc; YEAST:G3O-31924-MON; -.
DR EvolutionaryTrace; P36057; -.
DR PRO; PR:P36057; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P36057; protein.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IDA:SGD.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0005785; C:signal recognition particle receptor complex; IPI:SGD.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; ISS:SGD.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0005047; F:signal recognition particle binding; IMP:SGD.
DR GO; GO:0045047; P:protein targeting to ER; IMP:SGD.
DR GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IC:SGD.
DR GO; GO:0006617; P:SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition; IC:ComplexPortal.
DR CDD; cd04105; SR_beta; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR019009; SRP_receptor_beta_su.
DR PANTHER; PTHR11485:SF34; PTHR11485:SF34; 1.
DR Pfam; PF09439; SRPRB; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Endoplasmic reticulum; GTP-binding; Membrane;
KW Nucleotide-binding; Receptor; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..244
FT /note="Signal recognition particle receptor subunit beta"
FT /id="PRO_0000101230"
FT TRANSMEM 7..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT BINDING 45..53
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000269|PubMed:12654246"
FT BINDING 66..69
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000269|PubMed:12654246"
FT BINDING 90
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000269|PubMed:12654246"
FT BINDING 154..157
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000269|PubMed:12654246"
FT STRAND 40..44
FT /evidence="ECO:0007829|PDB:1NRJ"
FT HELIX 51..60
FT /evidence="ECO:0007829|PDB:1NRJ"
FT STRAND 73..75
FT /evidence="ECO:0007829|PDB:1NRJ"
FT HELIX 78..80
FT /evidence="ECO:0007829|PDB:1NRJ"
FT STRAND 84..87
FT /evidence="ECO:0007829|PDB:1NRJ"
FT HELIX 92..95
FT /evidence="ECO:0007829|PDB:1NRJ"
FT HELIX 96..105
FT /evidence="ECO:0007829|PDB:1NRJ"
FT HELIX 106..108
FT /evidence="ECO:0007829|PDB:1NRJ"
FT STRAND 109..117
FT /evidence="ECO:0007829|PDB:1NRJ"
FT HELIX 126..142
FT /evidence="ECO:0007829|PDB:1NRJ"
FT STRAND 149..154
FT /evidence="ECO:0007829|PDB:1NRJ"
FT HELIX 164..187
FT /evidence="ECO:0007829|PDB:1NRJ"
FT HELIX 214..216
FT /evidence="ECO:0007829|PDB:1NRJ"
FT STRAND 217..219
FT /evidence="ECO:0007829|PDB:1NRJ"
FT STRAND 221..225
FT /evidence="ECO:0007829|PDB:1NRJ"
FT TURN 228..231
FT /evidence="ECO:0007829|PDB:1NRJ"
FT HELIX 234..243
FT /evidence="ECO:0007829|PDB:1NRJ"
SQ SEQUENCE 244 AA; 26974 MW; EA4AE9126B2A04B5 CRC64;
MLSNTLIIAC LLVIGTTIAL IAVQKASSKT GIKQKSYQPS IIIAGPQNSG KTSLLTLLTT
DSVRPTVVSQ EPLSAADYDG SGVTLVDFPG HVKLRYKLSD YLKTRAKFVK GLIFMVDSTV
DPKKLTTTAE FLVDILSITE SSCENGIDIL IACNKSELFT ARPPSKIKDA LESEIQKVIE
RRKKSLNEVE RKINEEDYAE NTLDVLQSTD GFKFANLEAS VVAFEGSINK RKISQWREWI
DEKL
