ID   SRPC_PSEPU              Reviewed;         470 AA.
AC   O31101;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Solvent efflux pump outer membrane protein SrpC;
DE   Flags: Precursor;
GN   Name=srpC;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND EFFLUX PUMP SUBSTRATES.
RC   STRAIN=ATCC 700801 / S12;
RX   PubMed=9417051; DOI=10.1074/jbc.273.1.85;
RA   Kieboom J., Dennis J.J., de Bont J.A.M., Zylstra G.J.;
RT   "Identification and molecular characterization of an efflux pump involved
RT   in Pseudomonas putida S12 solvent tolerance.";
RL   J. Biol. Chem. 273:85-91(1998).
RN   [2]
RP   INDUCTION.
RC   STRAIN=ATCC 700801 / S12;
RX   PubMed=9852029; DOI=10.1128/jb.180.24.6769-6772.1998;
RA   Kieboom J., Dennis J.J., Zylstra G.J., de Bont J.A.M.;
RT   "Active efflux of organic solvents by Pseudomonas putida S12 is induced by
RT   solvents.";
RL   J. Bacteriol. 180:6769-6772(1998).
CC   -!- FUNCTION: The outer membrane component of an organic solvent efflux
CC       pump. Involved in export of a number of low log POW compounds including
CC       hexane (log POW 3.5), toluene (log POW 2.5) and dimethylphthalate (log
CC       POW 2.3). The solvent resistance phenotype has been postulated to
CC       depend on the operon expression level.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- INDUCTION: Low constitutive expression; the srpABC operon is further
CC       induced up to 17-fold by organic solvents (e.g. toluene and aliphatic
CC       solvents and alcohols) but not by antibiotics, heavy metals, pH,
CC       temperature, high NaCl or 60 mM acetic acid.
CC       {ECO:0000269|PubMed:9852029}.
CC   -!- SIMILARITY: Belongs to the outer membrane factor (OMF) (TC 1.B.17)
CC       family. {ECO:0000305}.
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DR   EMBL; AF029405; AAD12177.1; -; Genomic_DNA.
DR   RefSeq; WP_014003971.1; NZ_SPUU01000035.1.
DR   AlphaFoldDB; O31101; -.
DR   SMR; O31101; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR003423; OMP_efflux.
DR   InterPro; IPR010131; RND_efflux_OM_lipoprot_NodT.
DR   Pfam; PF02321; OEP; 2.
DR   TIGRFAMs; TIGR01845; outer_NodT; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   2: Evidence at transcript level;
KW   Cell outer membrane; Lipoprotein; Membrane; Palmitate; Signal;
KW   Transmembrane; Transmembrane beta strand; Transport.
FT   SIGNAL          1..16
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           17..470
FT                   /note="Solvent efflux pump outer membrane protein SrpC"
FT                   /id="PRO_0000031008"
FT   REGION          104..123
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        106..123
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   LIPID           17
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           17
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   470 AA;  51387 MW;  C67F5C669E9308D6 CRC64;
     MKFKSLPMFA LLMLGGCSLI PDYQQPAAPM QAQWPTGQAY GGQGDQRSIA TALPKAKEFF
     KDPALVRLLD AALENNRDLR IAAKNVESYR ALYRIQRAER FPTLDGQASG NRTRLPDDLS
     PTGDSRIDSQ YQVGLVTAYE LDLFGRIRSL SNQALEKYLA TEEAQRSVQI ALIGDVATTY
     FLWRTDQALL ELTEATLTSY VESLAMIESS AWAGTSSELD VRQARTLVNQ AQAQQALYTR
     RIAQDVNALE LLLGSKIPTD LPKNSPLAMS ALGKVPAGLP ADLLLNRPDI RSAEHQLMAA
     NANIGAARAA FFPRISLTAS AGSASSDLDG LFNSGSDSWS FAPQISVPIF NAGKLRANLD
     YAELQKDVGV ATYEKSIQTA FREVADGLAA RGTYGKQLSA QSELVDNYKA YFSLAQQRYD
     QGVDSYLTVL DAQRELFSSQ QKLLNDQLDQ INSEVQLYKA LGGGWSVSQN