SRPP_ARATH
ID SRPP_ARATH Reviewed; 165 AA.
AC O81417;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Protein SEED AND ROOT HAIR PROTECTIVE PROTEIN {ECO:0000303|PubMed:28138059};
DE AltName: Full=Protein ROOT HAIR SPECIFIC 13 {ECO:0000303|PubMed:19448035};
DE Flags: Precursor;
GN Name=SRPP {ECO:0000303|PubMed:28138059};
GN Synonyms=RHS13 {ECO:0000303|PubMed:19448035};
GN OrderedLocusNames=At4g02270 {ECO:0000312|Araport:AT4G02270};
GN ORFNames=T2H3.4 {ECO:0000312|EMBL:AAC28181.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=19448035; DOI=10.1104/pp.109.140905;
RA Won S.-K., Lee Y.-J., Lee H.-Y., Heo Y.-K., Cho M., Cho H.-T.;
RT "Cis-element- and transcriptome-based screening of root hair-specific genes
RT and their functional characterization in Arabidopsis.";
RL Plant Physiol. 150:1459-1473(2009).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP SUBCELLULAR LOCATION, AND INDUCTION BY PHOSPHATE DEPRIVATION.
RC STRAIN=cv. Columbia, cv. No-0, and cv. NR23;
RX PubMed=28138059; DOI=10.1093/pcp/pcx008;
RA Tanaka N., Uno H., Okuda S., Gunji S., Ferjani A., Aoyama T., Maeshima M.;
RT "SRPP, a Cell Wall Protein is Involved in Development and Protection of
RT Seeds and Root Hairs in Arabidopsis thaliana.";
RL Plant Cell Physiol. 58:760-769(2017).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, INDUCTION BY PHOSPHATE
RP DEPRIVATION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=28837399; DOI=10.1080/15592324.2017.1368940;
RA Uno H., Tanaka-Takada N., Sato R., Maeshima M.;
RT "Enhancement of cell wall protein SRPP expression during emergent root hair
RT development in Arabidopsis.";
RL Plant Signal. Behav. 12:e1368940-e1368940(2017).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia, and cv. No-0;
RX PubMed=30673938; DOI=10.1007/s10265-018-01083-6;
RA Uno H., Tanaka-Takada N., Hattori M., Fukuda M., Maeshima M.;
RT "A cell-wall protein SRPP provides physiological integrity to the
RT Arabidopsis seed.";
RL J. Plant Res. 132:145-154(2019).
CC -!- FUNCTION: Contributes to cell wall structure in root hairs and seeds,
CC especially in phosphate (Pi) deprivation conditions or in the presence
CC of ethylene (PubMed:28138059, PubMed:28837399). Particularly important
CC in maternal tissues (pericarps and seed coats) during seed development,
CC especially under stress conditions (PubMed:28138059, PubMed:30673938).
CC Confers thermotolerance in seed germination rate (PubMed:30673938).
CC {ECO:0000269|PubMed:28138059, ECO:0000269|PubMed:28837399,
CC ECO:0000269|PubMed:30673938}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC {ECO:0000269|PubMed:28138059}.
CC -!- TISSUE SPECIFICITY: Root hair and seed specific expression
CC (PubMed:19448035, PubMed:28138059, PubMed:28837399). Also observed in
CC other tissues including siliques, roots and flowers (PubMed:28138059,
CC PubMed:28837399). {ECO:0000269|PubMed:19448035,
CC ECO:0000269|PubMed:28138059, ECO:0000269|PubMed:28837399}.
CC -!- DEVELOPMENTAL STAGE: In roots, observed in root tips and elongating
CC regions of the primary and lateral roots, especially in root hairs and
CC collet hairs (PubMed:28138059). In reproductive organs, present in
CC fruits and anthers (mostly in young anthers) (PubMed:28138059). Later
CC detected in the pericarps and receptacles during maturation of siliques
CC (PubMed:28138059). In siliques, expressed in the embryo (from the
CC initial globular to mature stages), funiculus and seed coat (in the
CC transparent inner integument) (PubMed:28138059). Expression levels are
CC coordinated with root hair cell elongation (PubMed:28837399).
CC {ECO:0000269|PubMed:28138059, ECO:0000269|PubMed:28837399}.
CC -!- INDUCTION: Accumulates in root hairs within five days under phosphate
CC (Pi) deficient conditions (at protein level).
CC {ECO:0000269|PubMed:28138059, ECO:0000269|PubMed:28837399}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal conditions
CC (PubMed:28138059). Short and abnormally shaped root hairs due to the
CC suppression of cell growth and cell death, and withered seeds, both
CC having a tendency to necrotize and thick cell walls with abnormal
CC structure in phosphate (Pi) deficient conditions or after ethylene
CC treatment (PubMed:28138059, PubMed:28837399). Production of dark brown
CC shrunken seeds unable to germinate, especially in low humidity
CC (PubMed:30673938). {ECO:0000269|PubMed:28138059,
CC ECO:0000269|PubMed:28837399, ECO:0000269|PubMed:30673938}.
CC -!- SIMILARITY: Belongs to the plant proline-rich protein superfamily.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF075597; AAC28181.1; -; Genomic_DNA.
DR EMBL; AL161494; CAB80720.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE82149.1; -; Genomic_DNA.
DR EMBL; BT006378; AAP21186.1; -; mRNA.
DR EMBL; AK227972; BAE99939.1; -; mRNA.
DR PIR; T01421; T01421.
DR RefSeq; NP_192136.1; NM_116460.4.
DR AlphaFoldDB; O81417; -.
DR SMR; O81417; -.
DR STRING; 3702.AT4G02270.1; -.
DR PaxDb; O81417; -.
DR PRIDE; O81417; -.
DR ProteomicsDB; 177112; -.
DR EnsemblPlants; AT4G02270.1; AT4G02270.1; AT4G02270.
DR GeneID; 828083; -.
DR Gramene; AT4G02270.1; AT4G02270.1; AT4G02270.
DR KEGG; ath:AT4G02270; -.
DR Araport; AT4G02270; -.
DR TAIR; locus:2137814; AT4G02270.
DR eggNOG; ENOG502SZUR; Eukaryota.
DR HOGENOM; CLU_033592_0_0_1; -.
DR InParanoid; O81417; -.
DR OMA; QSTRTGC; -.
DR OrthoDB; 1370614at2759; -.
DR PhylomeDB; O81417; -.
DR PRO; PR:O81417; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O81417; baseline and differential.
DR GO; GO:0071944; C:cell periphery; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0009505; C:plant-type cell wall; IDA:TAIR.
DR GO; GO:0016036; P:cellular response to phosphate starvation; IMP:UniProtKB.
DR GO; GO:0009873; P:ethylene-activated signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0010286; P:heat acclimation; IMP:UniProtKB.
DR GO; GO:0009832; P:plant-type cell wall biogenesis; IMP:TAIR.
DR GO; GO:0009723; P:response to ethylene; IMP:UniProtKB.
DR GO; GO:0090547; P:response to low humidity; IMP:UniProtKB.
DR GO; GO:0080147; P:root hair cell development; IMP:UniProtKB.
DR GO; GO:0048316; P:seed development; IMP:UniProtKB.
DR GO; GO:0009826; P:unidimensional cell growth; IEP:UniProtKB.
PE 1: Evidence at protein level;
KW Cell wall; Developmental protein; Ethylene signaling pathway;
KW Reference proteome; Secreted; Signal; Stress response.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..165
FT /note="Protein SEED AND ROOT HAIR PROTECTIVE PROTEIN"
FT /id="PRO_5014306609"
SQ SEQUENCE 165 AA; 18103 MW; AFD19945C15706C7 CRC64;
MAFSRLSFAA SLIVFSSLII SSVAYYGNEA DPETGKLIPI AVEGIIKCKS GGKTYPIQGA
TARIACVKVD AYGNELVPIS ILSSKTDAKG YFIATIFPSQ LRAGRTVTKC KTYLYKSPLA
DCDFPTDVNK GVRGQPLSTY RILQDKSFKL YWAGPFFYTS EPTYY
