ID   SRRA_STAAC              Reviewed;         241 AA.
AC   Q5HFT0;
DT   27-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Transcriptional regulatory protein SrrA;
DE   AltName: Full=Staphylococcal respiratory response protein A;
GN   Name=srrA; OrderedLocusNames=SACOL1535;
OS   Staphylococcus aureus (strain COL).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93062;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=COL;
RX   PubMed=15774886; DOI=10.1128/jb.187.7.2426-2438.2005;
RA   Gill S.R., Fouts D.E., Archer G.L., Mongodin E.F., DeBoy R.T., Ravel J.,
RA   Paulsen I.T., Kolonay J.F., Brinkac L.M., Beanan M.J., Dodson R.J.,
RA   Daugherty S.C., Madupu R., Angiuoli S.V., Durkin A.S., Haft D.H.,
RA   Vamathevan J.J., Khouri H., Utterback T.R., Lee C., Dimitrov G., Jiang L.,
RA   Qin H., Weidman J., Tran K., Kang K.H., Hance I.R., Nelson K.E.,
RA   Fraser C.M.;
RT   "Insights on evolution of virulence and resistance from the complete genome
RT   analysis of an early methicillin-resistant Staphylococcus aureus strain and
RT   a biofilm-producing methicillin-resistant Staphylococcus epidermidis
RT   strain.";
RL   J. Bacteriol. 187:2426-2438(2005).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=16859493; DOI=10.1111/j.1365-2958.2006.05290.x;
RA   Richardson A.R., Dunman P.M., Fang F.C.;
RT   "The nitrosative stress response of Staphylococcus aureus is required for
RT   resistance to innate immunity.";
RL   Mol. Microbiol. 61:927-939(2006).
RN   [3]
RP   INDUCTION DURING ANAEROBIC GROWTH.
RX   PubMed=17384184; DOI=10.1128/jb.00081-07;
RA   Fuchs S., Pane-Farre J., Kohler C., Hecker M., Engelmann S.;
RT   "Anaerobic gene expression in Staphylococcus aureus.";
RL   J. Bacteriol. 189:4275-4289(2007).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=24222487; DOI=10.1128/mbio.00696-13;
RA   Kinkel T.L., Roux C.M., Dunman P.M., Fang F.C.;
RT   "The Staphylococcus aureus SrrAB two-component system promotes resistance
RT   to nitrosative stress and hypoxia.";
RL   MBio 4:E00696-E00713(2013).
CC   -!- FUNCTION: Member of the two-component regulatory system SrrA/SrrB,
CC       which is involved in the global regulation of staphylococcal virulence
CC       factors in response to environmental oxygen levels as well as biofilm
CC       formation (PubMed:24222487). Also plays an essential role in host-
CC       derived nitric oxide resistance by regulating hmp/flavohemoglobin, an
CC       enzyme that detoxifies nitric oxide by converting it to nitrate
CC       (PubMed:16859493, PubMed:24222487). Functions as a transcription
CC       regulator by direct binding to promoter regions of target genes (By
CC       similarity). {ECO:0000250|UniProtKB:Q9L524,
CC       ECO:0000269|PubMed:16859493, ECO:0000269|PubMed:24222487}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: Up-regulated during anaerobic growth.
CC       {ECO:0000269|PubMed:17384184}.
CC   -!- PTM: Phosphorylated by SrrB. {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Inactivation significantly enhances
CC       staphylococcal nitric oxide susceptibility.
CC       {ECO:0000269|PubMed:16859493, ECO:0000269|PubMed:24222487}.
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DR   EMBL; CP000046; AAW36728.1; -; Genomic_DNA.
DR   RefSeq; WP_000064078.1; NC_002951.2.
DR   AlphaFoldDB; Q5HFT0; -.
DR   SMR; Q5HFT0; -.
DR   EnsemblBacteria; AAW36728; AAW36728; SACOL1535.
DR   KEGG; sac:SACOL1535; -.
DR   HOGENOM; CLU_000445_30_4_9; -.
DR   OMA; NYEFFGD; -.
DR   Proteomes; UP000000530; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   2: Evidence at transcript level;
KW   Activator; Cytoplasm; DNA-binding; Phosphoprotein; Repressor;
KW   Transcription; Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..241
FT                   /note="Transcriptional regulatory protein SrrA"
FT                   /id="PRO_0000081248"
FT   DOMAIN          4..117
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        133..233
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         53
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   241 AA;  28161 MW;  491354FD9AED6F97 CRC64;
     MSNEILIVDD EDRIRRLLKM YLERESFEIH EASNGQEAYE LAMENNYACI LLDLMLPEMD
     GIQVATKLRE HKQTPIIMLT AKGEETNRVE GFESGADDYI VKPFSPREVV LRVKALLRRT
     QSTTVEQSEP HARDVIEFKH LEIDNDAHRV LADNQEVNLT PKEYELLIYL AKTPNKVFDR
     EQLLKEVWHY EFYGDLRTVD THVKRLREKL NRVSSEAAHM IQTVWGVGYK FEVKSNDEPA
     K