ID   SRRA_STAAU              Reviewed;         241 AA.
AC   Q9L524;
DT   27-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 2.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=Transcriptional regulatory protein SrrA;
DE   AltName: Full=Staphylococcal respiratory response protein A;
GN   Name=srrA;
OS   Staphylococcus aureus.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION.
RC   STRAIN=MN8;
RX   PubMed=11157922; DOI=10.1128/jb.183.4.1113-1123.2001;
RA   Yarwood J.M., McCormick J.K., Schlievert P.M.;
RT   "Identification of a novel two-component regulatory system that acts in
RT   global regulation of virulence factors of Staphylococcus aureus.";
RL   J. Bacteriol. 183:1113-1123(2001).
RN   [2]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=MN8;
RX   PubMed=15060046; DOI=10.1128/jb.186.8.2430-2438.2004;
RA   Pragman A.A., Yarwood J.M., Tripp T.J., Schlievert P.M.;
RT   "Characterization of virulence factor regulation by SrrAB, a two-component
RT   system in Staphylococcus aureus.";
RL   J. Bacteriol. 186:2430-2438(2004).
CC   -!- FUNCTION: Member of the two-component regulatory system SrrA/SrrB,
CC       which is involved in the global regulation of staphylococcal virulence
CC       factors in response to environmental oxygen levels as well as biofilm
CC       formation. Also plays an essential role in host-derived nitric oxide
CC       resistance by regulating hmp/flavohemoglobin, an enzyme that detoxifies
CC       nitric oxide by converting it to nitrate (By similarity). Functions as
CC       a transcription regulator by direct binding to promoter regions of
CC       target genes including agr, spa and tst promoters (PubMed:11157922).
CC       {ECO:0000250|UniProtKB:Q5HFT0, ECO:0000269|PubMed:11157922}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15060046}.
CC   -!- INDUCTION: Autoregulated. Expression is maximal during the
CC       postexponential phase of growth, particularly under microaerobic
CC       conditions. {ECO:0000269|PubMed:11157922}.
CC   -!- PTM: Phosphorylated by SrrB. {ECO:0000305}.
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DR   EMBL; AF260326; AAF70312.2; -; Genomic_DNA.
DR   PIR; D89928; D89928.
DR   RefSeq; WP_000064078.1; NZ_WYDB01000002.1.
DR   AlphaFoldDB; Q9L524; -.
DR   SMR; Q9L524; -.
DR   OMA; NYEFFGD; -.
DR   PHI-base; PHI:5466; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   2: Evidence at transcript level;
KW   Activator; Cytoplasm; DNA-binding; Phosphoprotein; Repressor;
KW   Transcription; Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..241
FT                   /note="Transcriptional regulatory protein SrrA"
FT                   /id="PRO_0000081247"
FT   DOMAIN          4..117
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        133..233
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         53
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   241 AA;  28161 MW;  491354FD9AED6F97 CRC64;
     MSNEILIVDD EDRIRRLLKM YLERESFEIH EASNGQEAYE LAMENNYACI LLDLMLPEMD
     GIQVATKLRE HKQTPIIMLT AKGEETNRVE GFESGADDYI VKPFSPREVV LRVKALLRRT
     QSTTVEQSEP HARDVIEFKH LEIDNDAHRV LADNQEVNLT PKEYELLIYL AKTPNKVFDR
     EQLLKEVWHY EFYGDLRTVD THVKRLREKL NRVSSEAAHM IQTVWGVGYK FEVKSNDEPA
     K