SRRM4_MOUSE
ID SRRM4_MOUSE Reviewed; 608 AA.
AC Q8BKA3; Q8R1S5; Q9CW37;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Serine/arginine repetitive matrix protein 4;
DE AltName: Full=Neural-specific serine/arginine repetitive splicing factor of 100 kDa;
DE Short=Neural-specific SR-related protein of 100 kDa;
DE Short=nSR100;
GN Name=Srrm4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, and Eye;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and FVB/N; TISSUE=Brain, and Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, RNA-BINDING, AND
RP PHOSPHORYLATION.
RX PubMed=19737518; DOI=10.1016/j.cell.2009.06.012;
RA Calarco J.A., Superina S., O'Hanlon D., Gabut M., Raj B., Pan Q.,
RA Skalska U., Clarke L., Gelinas D., van der Kooy D., Zhen M., Ciruna B.,
RA Blencowe B.J.;
RT "Regulation of vertebrate nervous system alternative splicing and
RT development by an SR-related protein.";
RL Cell 138:898-910(2009).
RN [4]
RP FUNCTION.
RX PubMed=21884984; DOI=10.1016/j.molcel.2011.08.014;
RA Raj B., O'Hanlon D., Vessey J.P., Pan Q., Ray D., Buckley N.J.,
RA Miller F.D., Blencowe B.J.;
RT "Cross-regulation between an alternative splicing activator and a
RT transcription repressor controls neurogenesis.";
RL Mol. Cell 43:843-850(2011).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, AND TISSUE
RP SPECIFICITY.
RX PubMed=25838543; DOI=10.1101/gad.256115.114;
RA Quesnel-Vallieres M., Irimia M., Cordes S.P., Blencowe B.J.;
RT "Essential roles for the splicing regulator nSR100/SRRM4 during nervous
RT system development.";
RL Genes Dev. 29:746-759(2015).
CC -!- FUNCTION: Splicing factor specifically required for neural cell
CC differentiation. Acts in conjunction with nPTB/PTBP2 by binding
CC directly to its regulated target transcripts and promotes neural-
CC specific exon inclusion in many genes that function in neural cell
CC differentiation. Required to promote the inclusion of neural-specific
CC exon 10 in nPTB/PTBP2, leading to increased expression of neural-
CC specific nPTB/PTBP2. Also promotes the inclusion of exon 16 in DAAM1 in
CC neuron extracts (PubMed:19737518). Promotes alternative splicing of
CC REST transcripts to produce REST isoform 2 (REST4) with greatly reduced
CC repressive activity, thereby activating expression of REST targets in
CC neural cells (PubMed:21884984). Plays an important role during
CC embryonic development as well as in the proper functioning of the adult
CC nervous system. Regulates alternative splicing events in genes with
CC important neuronal functions (PubMed:25838543).
CC {ECO:0000269|PubMed:19737518, ECO:0000269|PubMed:21884984,
CC ECO:0000269|PubMed:25838543}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:19737518}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BKA3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BKA3-2; Sequence=VSP_029639;
CC -!- TISSUE SPECIFICITY: Specifically expressed in neuronal cells (at
CC protein level). Expressed in adult nervous system and sensory organ
CC tissues. {ECO:0000269|PubMed:19737518, ECO:0000269|PubMed:25838543}.
CC -!- DEVELOPMENTAL STAGE: Expressed in both the central and peripheral
CC nervous system in developing mice. {ECO:0000269|PubMed:25838543}.
CC -!- PTM: Phosphorylated. {ECO:0000269|PubMed:19737518}.
CC -!- DISRUPTION PHENOTYPE: About 85% of the mutant mice die in the first few
CC hours after birth and few surviving animals display balance defects and
CC exhibit persistent tremors. Mice show impaired development of the
CC central and peripheral nervous systems in part by disrupting neurite
CC outgrowth, cortical layering in the forebrain, and axon guidance in the
CC corpus callosum. {ECO:0000269|PubMed:25838543}.
CC -!- SIMILARITY: Belongs to the nSR100 family. {ECO:0000305}.
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DR EMBL; AK005085; BAB23809.1; -; mRNA.
DR EMBL; AK053836; BAC35548.1; -; mRNA.
DR EMBL; BC024130; AAH24130.1; -; mRNA.
DR EMBL; BC056392; AAH56392.1; -; mRNA.
DR CCDS; CCDS19601.1; -. [Q8BKA3-1]
DR RefSeq; NP_081162.1; NM_026886.3. [Q8BKA3-1]
DR RefSeq; XP_006530519.1; XM_006530456.3. [Q8BKA3-1]
DR AlphaFoldDB; Q8BKA3; -.
DR BioGRID; 213139; 1.
DR IntAct; Q8BKA3; 1.
DR MINT; Q8BKA3; -.
DR STRING; 10090.ENSMUSP00000075488; -.
DR iPTMnet; Q8BKA3; -.
DR PhosphoSitePlus; Q8BKA3; -.
DR MaxQB; Q8BKA3; -.
DR PaxDb; Q8BKA3; -.
DR PRIDE; Q8BKA3; -.
DR ProteomicsDB; 257405; -. [Q8BKA3-1]
DR Antibodypedia; 56195; 55 antibodies from 15 providers.
DR DNASU; 68955; -.
DR Ensembl; ENSMUST00000076124; ENSMUSP00000075488; ENSMUSG00000063919. [Q8BKA3-1]
DR Ensembl; ENSMUST00000139425; ENSMUSP00000144367; ENSMUSG00000063919. [Q8BKA3-2]
DR GeneID; 68955; -.
DR KEGG; mmu:68955; -.
DR UCSC; uc008zfb.1; mouse. [Q8BKA3-2]
DR UCSC; uc008zfc.1; mouse. [Q8BKA3-1]
DR CTD; 84530; -.
DR MGI; MGI:1916205; Srrm4.
DR VEuPathDB; HostDB:ENSMUSG00000063919; -.
DR eggNOG; ENOG502QSPB; Eukaryota.
DR GeneTree; ENSGT00730000111247; -.
DR HOGENOM; CLU_032561_1_0_1; -.
DR InParanoid; Q8BKA3; -.
DR OMA; PRTEPQN; -.
DR PhylomeDB; Q8BKA3; -.
DR BioGRID-ORCS; 68955; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Srrm4; mouse.
DR PRO; PR:Q8BKA3; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8BKA3; protein.
DR Bgee; ENSMUSG00000063919; Expressed in embryonic brain and 108 other tissues.
DR ExpressionAtlas; Q8BKA3; baseline and differential.
DR Genevisible; Q8BKA3; MM.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0003729; F:mRNA binding; IDA:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IMP:UniProtKB.
DR GO; GO:0006397; P:mRNA processing; IDA:UniProtKB.
DR GO; GO:0007399; P:nervous system development; IMP:UniProtKB.
DR GO; GO:0042551; P:neuron maturation; IBA:GO_Central.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IMP:UniProtKB.
DR GO; GO:0043484; P:regulation of RNA splicing; IDA:UniProtKB.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR GO; GO:0007605; P:sensory perception of sound; IMP:MGI.
DR InterPro; IPR029360; SRRM_C.
DR Pfam; PF15230; SRRM_C; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Differentiation; mRNA processing; mRNA splicing;
KW Nucleus; Phosphoprotein; Reference proteome; RNA-binding.
FT CHAIN 1..608
FT /note="Serine/arginine repetitive matrix protein 4"
FT /id="PRO_0000311912"
FT REGION 34..246
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 261..608
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 80..94
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 107..187
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 198..217
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 273..305
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 318..343
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 371..417
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 424..456
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 457..479
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 491..508
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 517..608
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..488
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_029639"
SQ SEQUENCE 608 AA; 67860 MW; 16DBEF71B68C301C CRC64;
MASLQQGEKQ LFEKFWKGTF KAVATPRPES IIVASITARK PMPRTEPQSS LLLPDQDGPS
EKLGQHLAPE ALGTNSWGRE KACRELDPAR AHSASQDRDP TPPPSSRGKK KKKKSTRKKR
RRSPSYSPSP VKKKKKKSSK KHKRHRSFSK KRRHSSCSPK SKRREEKRHK KQSRSRKSHR
HRHHRCPSRS QSSELRSPSC ESRHRGRSPE EGRKSRRTHS RRCSKNHCKV SPDARSSHLP
SQPLPRLGFL SARGVITGSG SAADLFSKSA SPLAATRGRS QEYDSGNDTS SPPSTQTSSA
RSRGQEKGSP GGDLSKSRDL NCGNTSDSGN SFTTSSPQNK GAVLETVSPA CRSRESRGFQ
SPCLQCAEVK KSSLVPSTAR SSPIKECSRS SSYTSTRSSS PSSRSPNPRA SPRYTRSRST
SSEKRSYSRS PSYSSKSGKR SPPSRSSRSR RSPSYSRYSP SRERDLKYGE KEPQPRERAR
RRRRSYSPMR KRRRDSPSHL EARRITSARK RPIPYYRPSP SSSSSLSSAS SWYSSSSSSS
SSSSRSPSRS YSRSRSPSRS HSSRSQTRSR TRTSRSSSSR SLSLGSRSRS RNRSLSYSSA
ESYASTRR
