SRS12_HUMAN
ID SRS12_HUMAN Reviewed; 261 AA.
AC Q8WXF0; B2RA22; Q5T7K0; Q8WW25;
DT 24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Serine/arginine-rich splicing factor 12;
DE AltName: Full=35 kDa SR repressor protein;
DE Short=SRrp35;
DE AltName: Full=Splicing factor, arginine/serine-rich 13B;
DE AltName: Full=Splicing factor, arginine/serine-rich 19;
GN Name=SRSF12; Synonyms=SFRS13B, SFRS19, SRRP35;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=11684676; DOI=10.1074/jbc.m103967200;
RA Cowper A.E., Caceres J.F., Mayeda A., Screaton G.R.;
RT "Serine-arginine (SR) protein-like factors that antagonize authentic SR
RT proteins and regulate alternative splicing.";
RL J. Biol. Chem. 276:48908-48914(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Splicing factor that seems to antagonize SR proteins in pre-
CC mRNA splicing regulation. {ECO:0000269|PubMed:11684676}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in testis. {ECO:0000269|PubMed:11684676}.
CC -!- SIMILARITY: Belongs to the splicing factor SR family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAW48561.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF449428; AAL57515.1; -; mRNA.
DR EMBL; AL353135; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471051; EAW48561.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AK314008; BAG36719.1; -; mRNA.
DR EMBL; BC021715; AAH21715.1; -; mRNA.
DR CCDS; CCDS47459.1; -.
DR RefSeq; NP_542781.3; NM_080743.4.
DR RefSeq; XP_016865781.1; XM_017010292.1.
DR AlphaFoldDB; Q8WXF0; -.
DR SMR; Q8WXF0; -.
DR BioGRID; 126428; 127.
DR IntAct; Q8WXF0; 32.
DR STRING; 9606.ENSP00000414302; -.
DR GlyGen; Q8WXF0; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q8WXF0; -.
DR PhosphoSitePlus; Q8WXF0; -.
DR SwissPalm; Q8WXF0; -.
DR BioMuta; SRSF12; -.
DR DMDM; 47606193; -.
DR EPD; Q8WXF0; -.
DR jPOST; Q8WXF0; -.
DR MassIVE; Q8WXF0; -.
DR MaxQB; Q8WXF0; -.
DR PaxDb; Q8WXF0; -.
DR PeptideAtlas; Q8WXF0; -.
DR PRIDE; Q8WXF0; -.
DR ProteomicsDB; 75022; -.
DR Antibodypedia; 54701; 26 antibodies from 12 providers.
DR DNASU; 135295; -.
DR Ensembl; ENST00000452027.3; ENSP00000414302.2; ENSG00000154548.9.
DR GeneID; 135295; -.
DR KEGG; hsa:135295; -.
DR MANE-Select; ENST00000452027.3; ENSP00000414302.2; NM_080743.5; NP_542781.3.
DR UCSC; uc063qaq.1; human.
DR CTD; 135295; -.
DR DisGeNET; 135295; -.
DR GeneCards; SRSF12; -.
DR HGNC; HGNC:21220; SRSF12.
DR HPA; ENSG00000154548; Tissue enhanced (testis).
DR neXtProt; NX_Q8WXF0; -.
DR OpenTargets; ENSG00000154548; -.
DR PharmGKB; PA165618223; -.
DR VEuPathDB; HostDB:ENSG00000154548; -.
DR eggNOG; KOG0118; Eukaryota.
DR GeneTree; ENSGT00940000160519; -.
DR HOGENOM; CLU_012062_10_2_1; -.
DR InParanoid; Q8WXF0; -.
DR OMA; CSPADHR; -.
DR OrthoDB; 1533297at2759; -.
DR PhylomeDB; Q8WXF0; -.
DR TreeFam; TF351864; -.
DR PathwayCommons; Q8WXF0; -.
DR SignaLink; Q8WXF0; -.
DR BioGRID-ORCS; 135295; 9 hits in 1068 CRISPR screens.
DR GenomeRNAi; 135295; -.
DR Pharos; Q8WXF0; Tbio.
DR PRO; PR:Q8WXF0; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q8WXF0; protein.
DR Bgee; ENSG00000154548; Expressed in cortical plate and 115 other tissues.
DR ExpressionAtlas; Q8WXF0; baseline and differential.
DR Genevisible; Q8WXF0; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0016607; C:nuclear speck; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR GO; GO:0050733; F:RS domain binding; NAS:UniProtKB.
DR GO; GO:0051082; F:unfolded protein binding; NAS:UniProtKB.
DR GO; GO:0000395; P:mRNA 5'-splice site recognition; IDA:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0048025; P:negative regulation of mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; IDA:UniProtKB.
DR GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; NAS:UniProtKB.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR034477; SRSF12.
DR PANTHER; PTHR23147:SF3; PTHR23147:SF3; 1.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 2: Evidence at transcript level;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome; RNA-binding.
FT CHAIN 1..261
FT /note="Serine/arginine-rich splicing factor 12"
FT /id="PRO_0000081960"
FT DOMAIN 10..88
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 86..261
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 93..113
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 128..146
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 150..171
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 172..188
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 189..210
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 243..261
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 63
FT /note="D -> G (in Ref. 5; AAH21715)"
FT /evidence="ECO:0000305"
FT CONFLICT 149
FT /note="Q -> K (in Ref. 5; AAH21715)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 261 AA; 30512 MW; C1473A5760462E54 CRC64;
MSRYTRPPNT SLFIRNVADA TRPEDLRREF GRYGPIVDVY IPLDFYTRRP RGFAYVQFED
VRDAEDALYN LNRKWVCGRQ IEIQFAQGDR KTPGQMKSKE RHPCSPSDHR RSRSPSQRRT
RSRSSSWGRN RRRSDSLKES RHRRFSYSQS KSRSKSLPRR STSARQSRTP RRNFGSRGRS
RSKSLQKRSK SIGKSQSSSP QKQTSSGTKS RSHGRHSDSI ARSPCKSPKG YTNSETKVQT
AKHSHFRSHS RSRSYRHKNS W
