SRS1B_DANRE
ID SRS1B_DANRE Reviewed; 245 AA.
AC Q6NYA0; Q502R8; Q6PH14;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Serine/arginine-rich splicing factor 1B;
DE AltName: Full=Splicing factor, arginine/serine-rich 1;
DE AltName: Full=Splicing factor, arginine/serine-rich 1B;
GN Name=srsf1b; Synonyms=sfrs1, sfrs1b;
GN ORFNames=zgc:111894, zgc:65898, zgc:76897;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo, and Olfactory epithelium;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 64-245.
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
CC -!- FUNCTION: May play a role in preventing exon skipping, ensuring the
CC accuracy of splicing and regulating alternative splicing.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q07955}. Nucleus
CC speckle {ECO:0000250|UniProtKB:Q07955}. Note=In nuclear speckles.
CC Shuttles between the nucleus and the cytoplasm.
CC {ECO:0000250|UniProtKB:Q07955}.
CC -!- SIMILARITY: Belongs to the splicing factor SR family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH56752.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR EMBL; BC056752; AAH56752.1; ALT_SEQ; mRNA.
DR EMBL; BC066682; AAH66682.1; -; mRNA.
DR EMBL; BC095586; AAH95586.1; -; mRNA.
DR EMBL; AL929224; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_956887.2; NM_200593.2.
DR AlphaFoldDB; Q6NYA0; -.
DR SMR; Q6NYA0; -.
DR STRING; 7955.ENSDARP00000102203; -.
DR PaxDb; Q6NYA0; -.
DR Ensembl; ENSDART00000143566; ENSDARP00000116886; ENSDARG00000017843.
DR GeneID; 393565; -.
DR KEGG; dre:393565; -.
DR CTD; 393565; -.
DR ZFIN; ZDB-GENE-040426-1467; srsf1b.
DR eggNOG; KOG0105; Eukaryota.
DR GeneTree; ENSGT00940000155585; -.
DR HOGENOM; CLU_012062_34_0_1; -.
DR InParanoid; Q6NYA0; -.
DR OrthoDB; 1321443at2759; -.
DR PhylomeDB; Q6NYA0; -.
DR Reactome; R-DRE-159236; Transport of Mature mRNA derived from an Intron-Containing Transcript.
DR Reactome; R-DRE-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-DRE-72165; mRNA Splicing - Minor Pathway.
DR Reactome; R-DRE-72187; mRNA 3'-end processing.
DR Reactome; R-DRE-73856; RNA Polymerase II Transcription Termination.
DR PRO; PR:Q6NYA0; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 21.
DR Bgee; ENSDARG00000017843; Expressed in testis and 26 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016607; C:nuclear speck; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0000380; P:alternative mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR CDD; cd12597; RRM1_SRSF1; 1.
DR Gene3D; 3.30.70.330; -; 2.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR034520; SRSF1_RRM1.
DR Pfam; PF00076; RRM_1; 2.
DR SMART; SM00360; RRM; 2.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 2.
PE 2: Evidence at transcript level;
KW Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Repeat; RNA-binding.
FT CHAIN 1..245
FT /note="Serine/arginine-rich splicing factor 1B"
FT /id="PRO_0000081914"
FT DOMAIN 15..90
FT /note="RRM 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT DOMAIN 120..194
FT /note="RRM 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 89..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 192..245
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 204..222
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 231..245
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 53
FT /note="P -> Q (in Ref. 1; AAH95586)"
FT /evidence="ECO:0000305"
FT CONFLICT 216
FT /note="S -> G (in Ref. 1; AAH95586)"
FT /evidence="ECO:0000305"
FT CONFLICT 244..245
FT /note="RT -> PVCFLSSLVCWVFMI (in Ref. 1; AAH95586)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 245 AA; 27475 MW; 99F04687D0AEA25F CRC64;
MSGGVIRGPA GNNDCRIYVG NLPPDIRTKD VEDVFYKYGA IRDIDLKNRR GGPPFAFVEF
EDPRDAEDAV YGRDGYDYDG YRLRVEFPRS GRGGGRGGGG GGGVGAPRGR YGPPSRRSEY
RVIVSGLPPS GSWQDLKDHM REAGDVCYAD VFRDGTGVVE FVRKEDMTYA VRKLDNTKFR
SHEGETAYIR VKVDGPRSPS YGRSRSRSRS RSRSRSNSRS RSYSPRRSRG SPRYSPRHSR
SRSRT
