SRSA_DICDI
ID SRSA_DICDI Reviewed; 106 AA.
AC B0G168; A2V850;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 1.
DT 25-MAY-2022, entry version 56.
DE RecName: Full=Starvation responsive small protein A {ECO:0000303|PubMed:18673382};
GN Name=srsA {ECO:0000303|PubMed:18673382};
GN ORFNames=DDB_G0289521 {ECO:0000312|EMBL:EDR41041.1,
GN ECO:0000312|EMBL:EDR41042.1};
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689 {ECO:0000312|Proteomes:UP000002195};
RN [1] {ECO:0000312|EMBL:BAF47287.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, INDUCTION, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=AX2 {ECO:0000303|PubMed:18673382};
RX PubMed=18673382; DOI=10.1111/j.1432-0436.2008.00298.x;
RA Sasaki K., Chae S.C., Loomis W.F., Iranfar N., Amagai A., Maeda Y.;
RT "An immediate-early gene, srsA: its involvement in the starvation response
RT that initiates differentiation of Dictyostelium cells.";
RL Differentiation 76:1093-1103(2008).
RN [2] {ECO:0000312|EMBL:EDR41041.1, ECO:0000312|EMBL:EDR41042.1, ECO:0000312|Proteomes:UP000002195}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4 {ECO:0000312|EMBL:EDR41041.1, ECO:0000312|EMBL:EDR41042.1,
RC ECO:0000312|Proteomes:UP000002195};
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Involved in starvation response and aggregation stage of the
CC life cycle. May be involved in fruiting body morphogenesis and spore
CC formation. {ECO:0000269|PubMed:18673382}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=B0G168-1; Sequence=Displayed;
CC Name=2;
CC IsoId=B0G168-2; Sequence=VSP_059868, VSP_059869;
CC Name=3;
CC IsoId=B0G168-3; Sequence=VSP_059870;
CC -!- INDUCTION: By starvation. Rapidly and transiently expressed in the
CC first 5 min following the removal of nutrients. Expression declines
CC after 30 min and is undetectable 2 hr after nutrient removal.
CC {ECO:0000269|PubMed:18673382}.
CC -!- DISRUPTION PHENOTYPE: Cells show no aggregation after 11.5 hr of
CC starvation under submerged conditions in contrast to the wild-type.
CC Cells developed on agar exhibit 2 hr delay in aggregation and the
CC aggregation streams formed after 9 hr are larger than normal leading to
CC over-sized mounds. No significant difference in the time of conversion
CC of mounds to migrating slugs, but the larger slugs formed by the mutant
CC cells often subdivide before forming normal-sized fruiting bodies with
CC an apparently normal proportion of spores and stalk. Grp94 transcript
CC decreases more slowly following starvation compared to rapid within 30
CC min decrease of it in wild-type. Significantly reduced and delayed
CC expression of the aggregation stage adenylate cyclase acaA and cyclic
CC AMP receptor carA genes. Both are normally expressed shortly after the
CC initiation of differentiation upon starvation and they are necessary
CC for chemotactic aggregation. {ECO:0000269|PubMed:18673382}.
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DR EMBL; AB292732; BAF47287.1; -; mRNA.
DR EMBL; AAFI02000141; EDR41041.1; -; Genomic_DNA.
DR EMBL; AAFI02000141; EDR41042.1; -; Genomic_DNA.
DR RefSeq; XP_001733031.1; XM_001732979.1.
DR RefSeq; XP_001733032.1; XM_001732980.1.
DR AlphaFoldDB; B0G168; -.
DR STRING; 44689.DDB0237692; -.
DR PaxDb; B0G168; -.
DR EnsemblProtists; EDR41041; EDR41041; DDB_G0289521.
DR EnsemblProtists; EDR41042; EDR41042; DDB_G0289521.
DR GeneID; 8627189; -.
DR KEGG; ddi:DDB_G0289521; -.
DR dictyBase; DDB_G0289521; srsA.
DR eggNOG; ENOG502RIIM; Eukaryota.
DR HOGENOM; CLU_2228248_0_0_1; -.
DR InParanoid; B0G168; -.
DR OMA; RGSQLAN; -.
DR PRO; PR:B0G168; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031152; P:aggregation involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0098743; P:cell aggregation; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR GO; GO:0042594; P:response to starvation; IEP:UniProtKB.
DR GO; GO:0099120; P:socially cooperative development; IEP:UniProtKB.
DR GO; GO:0031288; P:sorocarp morphogenesis; IMP:dictyBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IMP:dictyBase.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Membrane; Reference proteome;
KW Stress response; Transmembrane; Transmembrane helix.
FT CHAIN 1..106
FT /note="Starvation responsive small protein A"
FT /id="PRO_0000445439"
FT TRANSMEM 15..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 42..57
FT /note="VDEKAHIGIGHFFKKR -> NLITNFFNSNNNNKKQ (in isoform 2)"
FT /evidence="ECO:0000269|PubMed:18673382"
FT /id="VSP_059868"
FT VAR_SEQ 58..106
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000269|PubMed:18673382"
FT /id="VSP_059869"
FT VAR_SEQ 71..106
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_059870"
SQ SEQUENCE 106 AA; 12100 MW; 1AF141A078E205BE CRC64;
MIAKVFKSIT PESYILLVNA GLISAYGVRI IFQSVKNDEG KVDEKAHIGI GHFFKKRGAQ
LVDGKNLTDI LKEKKKKKKK KKKINISNKL IIFKISRQLY SKLKLK
