SRSF3_BOVIN
ID SRSF3_BOVIN Reviewed; 164 AA.
AC Q3SZR8;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Serine/arginine-rich splicing factor 3;
DE AltName: Full=Splicing factor, arginine/serine-rich 3;
GN Name=SRSF3; Synonyms=SFRS3;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Crossbred X Angus; TISSUE=Liver;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Splicing factor that specifically promotes exon-inclusion
CC during alternative splicing. Interaction with YTHDC1, a RNA-binding
CC protein that recognizes and binds N6-methyladenosine (m6A)-containing
CC RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements
CC adjacent to m6A sites, leading to exon-inclusion during alternative
CC splicing. Also functions as export adapter involved in mRNA nuclear
CC export. Binds mRNA which is thought to be transferred to the NXF1-NXT1
CC heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-
CC binding activity. Involved in nuclear export of m6A-containing mRNAs
CC via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-
CC containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear
CC export. RNA-binding is semi-sequence specific.
CC {ECO:0000250|UniProtKB:P84103}.
CC -!- SUBUNIT: Interacts with CPSF6. Interacts with RBMY1A1. Interacts with
CC SREK1/SFRS12. Interacts with NXF1. Interacts with YTHDC1, leading to
CC recruitment to RNA elements adjacent to m6A sites.
CC {ECO:0000250|UniProtKB:P84103}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P84103}. Nucleus
CC speckle {ECO:0000250|UniProtKB:P84103}. Cytoplasm
CC {ECO:0000250|UniProtKB:P84103}. Note=Recruited to nuclear speckles
CC following interaction with YTHDC1. {ECO:0000250|UniProtKB:P84103}.
CC -!- PTM: Phosphorylated by CLK1, CLK2, CLK3 and CLK4. Extensively
CC phosphorylated on serine residues in the RS domain.
CC {ECO:0000250|UniProtKB:P84104}.
CC -!- SIMILARITY: Belongs to the splicing factor SR family. {ECO:0000305}.
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DR EMBL; BC102735; AAI02736.1; -; mRNA.
DR RefSeq; NP_001029872.1; NM_001034700.2.
DR AlphaFoldDB; Q3SZR8; -.
DR SMR; Q3SZR8; -.
DR IntAct; Q3SZR8; 1.
DR STRING; 9913.ENSBTAP00000049550; -.
DR PaxDb; Q3SZR8; -.
DR PeptideAtlas; Q3SZR8; -.
DR PRIDE; Q3SZR8; -.
DR Ensembl; ENSBTAT00000051953; ENSBTAP00000049550; ENSBTAG00000040006.
DR GeneID; 540276; -.
DR KEGG; bta:540276; -.
DR CTD; 6428; -.
DR VEuPathDB; HostDB:ENSBTAG00000040006; -.
DR VGNC; VGNC:35301; SRSF3.
DR eggNOG; KOG0107; Eukaryota.
DR GeneTree; ENSGT00910000144115; -.
DR HOGENOM; CLU_012062_20_2_1; -.
DR InParanoid; Q3SZR8; -.
DR OMA; EMHRDSC; -.
DR OrthoDB; 1533297at2759; -.
DR TreeFam; TF351858; -.
DR Proteomes; UP000009136; Chromosome 23.
DR Bgee; ENSBTAG00000040006; Expressed in spermatid and 108 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016607; C:nuclear speck; ISS:UniProtKB.
DR GO; GO:0043274; F:phospholipase binding; IEA:Ensembl.
DR GO; GO:0070878; F:primary miRNA binding; IEA:Ensembl.
DR GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR GO; GO:1990830; P:cellular response to leukemia inhibitory factor; IEA:Ensembl.
DR GO; GO:0006406; P:mRNA export from nucleus; ISS:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR GO; GO:0031053; P:primary miRNA processing; IEA:Ensembl.
DR GO; GO:0048024; P:regulation of mRNA splicing, via spliceosome; ISS:UniProtKB.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Cytoplasm; mRNA processing; mRNA splicing; mRNA transport;
KW Nucleus; Phosphoprotein; Reference proteome; Repeat; RNA-binding;
KW Transport.
FT CHAIN 1..164
FT /note="Serine/arginine-rich splicing factor 3"
FT /id="PRO_0000287723"
FT DOMAIN 10..83
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REPEAT 119..133
FT /note="B-1"
FT REPEAT 149..164
FT /note="B-2"
FT REGION 1..90
FT /note="Sufficient for interaction with NXF1"
FT /evidence="ECO:0000250"
FT REGION 81..164
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 119..164
FT /note="2 X approximate repeats, basic"
FT COMPBIAS 95..109
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 110..124
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 125..164
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:P84103"
FT MOD_RES 5
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P84103"
FT MOD_RES 23
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:P84103"
SQ SEQUENCE 164 AA; 19330 MW; 02F0A5EE33FF28A0 CRC64;
MHRDSCPLDC KVYVGNLGNN GNKTELERAF GYYGPLRSVW VARNPPGFAF VEFEDPRDAA
DAVRELDGRT LCGCRVRVEL SNGEKRSRNR GPPPSWGRRP RDDYRRRSPP PRRRSPRRRS
FSRSRSRSLS RDRRRERSLS RERNHKPSRS FSRSRSRSRS NERK
