SRYB_DROME
ID SRYB_DROME Reviewed; 356 AA.
AC P07665; Q9VAB5;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2005, sequence version 2.
DT 03-AUG-2022, entry version 184.
DE RecName: Full=Serendipity locus protein beta;
GN Name=Sry-beta; Synonyms=Sry-b; ORFNames=CG7938;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=Oregon-R;
RX PubMed=3935797; DOI=10.1016/0022-2836(85)90265-7;
RA Vincent A., Colot H.V., Rosbash M.;
RT "Sequence and structure of the serendipity locus of Drosophila
RT melanogaster. A densely transcribed region including a blastoderm-specific
RT gene.";
RL J. Mol. Biol. 186:149-166(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP DNA-BINDING, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=2081456; DOI=10.1242/dev.110.1.141;
RA Payre F., Noselli S., Lefrere V., Vincent A.;
RT "The closely related Drosophila sry beta and sry delta zinc finger proteins
RT show differential embryonic expression and distinct patterns of binding
RT sites on polytene chromosomes.";
RL Development 110:141-149(1990).
RN [6]
RP DNA-BINDING SPECIFICITY.
RX PubMed=1868833; DOI=10.1002/j.1460-2075.1991.tb07793.x;
RA Payre F., Vincent A.;
RT "Genomic targets of the serendipity beta and delta zinc finger proteins and
RT their respective DNA recognition sites.";
RL EMBO J. 10:2533-2541(1991).
RN [7]
RP DNA-BINDING SPECIFICITY.
RX PubMed=1732741; DOI=10.1128/mcb.12.2.724-733.1992;
RA Noselli S., Payre F., Vincent A.;
RT "Zinc fingers and other domains cooperate in binding of Drosophila sry beta
RT and delta proteins at specific chromosomal sites.";
RL Mol. Cell. Biol. 12:724-733(1992).
CC -!- FUNCTION: Binds to the consensus DNA sequence 5'-YCAGAGATGCGCA-3'.
CC -!- SUBUNIT: Binds chromatin; requires N-terminal regions to form protein-
CC protein contacts, in addition to DNA specific recognition by the zinc
CC fingers.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:2081456}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC Abundant in early embryos and present in very low amounts at every
CC stage of the life-cycle. {ECO:0000269|PubMed:2081456,
CC ECO:0000269|PubMed:3935797}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA26896.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; X03121; CAA26896.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AE014297; AAF56998.1; -; Genomic_DNA.
DR EMBL; AY118322; AAM48351.1; -; mRNA.
DR PIR; A23351; A23351.
DR RefSeq; NP_001303451.1; NM_001316522.1.
DR RefSeq; NP_524579.1; NM_079840.3.
DR AlphaFoldDB; P07665; -.
DR SMR; P07665; -.
DR BioGRID; 68426; 20.
DR IntAct; P07665; 14.
DR STRING; 7227.FBpp0084922; -.
DR PaxDb; P07665; -.
DR PRIDE; P07665; -.
DR EnsemblMetazoa; FBtr0085556; FBpp0084922; FBgn0003511.
DR EnsemblMetazoa; FBtr0346861; FBpp0312401; FBgn0003511.
DR GeneID; 43570; -.
DR KEGG; dme:Dmel_CG7938; -.
DR CTD; 43570; -.
DR FlyBase; FBgn0003511; Sry-beta.
DR VEuPathDB; VectorBase:FBgn0003511; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000162287; -.
DR HOGENOM; CLU_776751_0_0_1; -.
DR InParanoid; P07665; -.
DR OMA; LECRYCS; -.
DR PhylomeDB; P07665; -.
DR BioGRID-ORCS; 43570; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 43570; -.
DR PRO; PR:P07665; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0003511; Expressed in stage 5 embryo (Drosophila) and 23 other tissues.
DR Genevisible; P07665; DM.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005705; C:polytene chromosome interband; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 3.
DR SMART; SM00355; ZnF_C2H2; 6.
DR SUPFAM; SSF57667; SSF57667; 3.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 5.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 5.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..356
FT /note="Serendipity locus protein beta"
FT /id="PRO_0000047049"
FT ZN_FING 171..193
FT /note="C2H2-type 1; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 201..223
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 229..251
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 257..279
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 286..308
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 315..337
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
SQ SEQUENCE 356 AA; 41709 MW; 6B5F2206A20CE8D4 CRC64;
MSSTRPFCFV CGKEKSVGVF QLIEGCIVPG TFKPIKDILK YFEKIINQRL ELLPNSAACR
DCLEYLFNYD RLVRNLSQVQ RQIADALLGC RQVEGKAETK QQAAKRARVQ VPAFKIVQAT
ALKEPERQPG EEDECEEFMK EEMLDEEFQF SEPDDSMPSS EEEFFTETTE IPCHICGEMF
SSQEVLERHI KADTCQKSEQ ATCNVCGLKV KDDEVLDLHM NLHEGKTELE CRYCDKKFSH
KRNVLRHMEV HWDKKKYQCD KCGERFSLSW LMYNHLMRHD AEENALICEV CHQQFKTKRT
YKHHLRTHQT DRPRYPCPDC EKSFVDKYTL KVHKRVHQPV EKPESAEAKE ATVTFF
