SRYD_DROME
ID SRYD_DROME Reviewed; 433 AA.
AC P07664; Q9VAB3;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2005, sequence version 2.
DT 03-AUG-2022, entry version 192.
DE RecName: Full=Serendipity locus protein delta;
GN Name=Sry-delta; Synonyms=Sry-d; ORFNames=CG17958;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=Oregon-R;
RX PubMed=3935797; DOI=10.1016/0022-2836(85)90265-7;
RA Vincent A., Colot H.V., Rosbash M.;
RT "Sequence and structure of the serendipity locus of Drosophila
RT melanogaster. A densely transcribed region including a blastoderm-specific
RT gene.";
RL J. Mol. Biol. 186:149-166(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 339-433, AND DEVELOPMENTAL STAGE.
RX PubMed=6329730; DOI=10.1002/j.1460-2075.1984.tb01920.x;
RA Vincent A., O'Connell P., Gray M.R., Rosbash M.;
RT "Drosophila maternal and embryo mRNAs transcribed from a single
RT transcription unit use alternate combinations of exons.";
RL EMBO J. 3:1003-1013(1984).
RN [6]
RP DNA-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=2081456; DOI=10.1242/dev.110.1.141;
RA Payre F., Noselli S., Lefrere V., Vincent A.;
RT "The closely related Drosophila sry beta and sry delta zinc finger proteins
RT show differential embryonic expression and distinct patterns of binding
RT sites on polytene chromosomes.";
RL Development 110:141-149(1990).
RN [7]
RP NUCLEAR LOCALIZATION SIGNAL.
RX PubMed=1849091; DOI=10.1016/0014-5793(91)80229-v;
RA Noselli S., Vincent A.;
RT "A Drosophila nuclear localisation signal included in an 18 amino acid
RT fragment from the serendipity delta zinc finger protein.";
RL FEBS Lett. 280:167-170(1991).
RN [8]
RP DNA-BINDING SPECIFICITY.
RX PubMed=1868833; DOI=10.1002/j.1460-2075.1991.tb07793.x;
RA Payre F., Vincent A.;
RT "Genomic targets of the serendipity beta and delta zinc finger proteins and
RT their respective DNA recognition sites.";
RL EMBO J. 10:2533-2541(1991).
RN [9]
RP FUNCTION, DNA-BINDING, AND HOMODIMERIZATION.
RX PubMed=9154812; DOI=10.1128/mcb.17.6.3137;
RA Payre F., Buono P., Vanzo N., Vincent A.;
RT "Two types of zinc fingers are required for dimerization of the serendipity
RT delta transcriptional activator.";
RL Mol. Cell. Biol. 17:3137-3145(1997).
RN [10]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=9858707; DOI=10.1016/s0925-4773(98)00159-2;
RA Ruez C., Payre F., Vincent A.;
RT "Transcriptional control of Drosophila bicoid by Serendipity delta:
RT cooperative binding sites, promoter context, and co-evolution.";
RL Mech. Dev. 78:125-134(1998).
CC -!- FUNCTION: Transcriptional activator that controls bicoid gene
CC expression during oogenesis. Found in transcriptionally active cells.
CC Binds to specific sites on polytene chromosomes of third instar larvae.
CC Binds to the consensus DNA sequence 5'-YTAGAGATGGRAA-3'.
CC {ECO:0000269|PubMed:9154812, ECO:0000269|PubMed:9858707}.
CC -!- SUBUNIT: Binds DNA as a homodimer. N-terminal regions of the protein
CC are required, in addition to the zinc fingers, for the specificity of
CC chromatin-binding.
CC -!- INTERACTION:
CC P07664; Q7JN06: BEAF-32; NbExp=4; IntAct=EBI-498092, EBI-134484;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:2081456}.
CC -!- TISSUE SPECIFICITY: Predominantly localized to the sub- and
CC supraesophagal ganglia and the ventral nerve cord in the embryo, after
CC dorsal closure. {ECO:0000269|PubMed:2081456}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically. Found
CC in ovaries and abundant in early embryos. Also found in variable
CC amounts at every stage of the life cycle. {ECO:0000269|PubMed:2081456,
CC ECO:0000269|PubMed:3935797, ECO:0000269|PubMed:6329730}.
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DR EMBL; X03121; CAA26898.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF57000.1; -; Genomic_DNA.
DR EMBL; AY069691; AAL39836.1; -; mRNA.
DR EMBL; X00555; CAA25221.1; -; Genomic_DNA.
DR PIR; C23351; C23351.
DR RefSeq; NP_524581.1; NM_079842.3.
DR PDB; 7POH; X-ray; 2.85 A; A/B=1-90.
DR PDBsum; 7POH; -.
DR AlphaFoldDB; P07664; -.
DR SMR; P07664; -.
DR BioGRID; 68428; 10.
DR IntAct; P07664; 8.
DR STRING; 7227.FBpp0084924; -.
DR PaxDb; P07664; -.
DR DNASU; 43572; -.
DR EnsemblMetazoa; FBtr0085558; FBpp0084924; FBgn0003512.
DR GeneID; 43572; -.
DR KEGG; dme:Dmel_CG17958; -.
DR CTD; 43572; -.
DR FlyBase; FBgn0003512; Sry-delta.
DR VEuPathDB; VectorBase:FBgn0003512; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000164700; -.
DR HOGENOM; CLU_776751_0_0_1; -.
DR InParanoid; P07664; -.
DR OMA; HINEDHS; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; P07664; -.
DR BioGRID-ORCS; 43572; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 43572; -.
DR PRO; PR:P07664; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0003512; Expressed in ovary and 11 other tissues.
DR Genevisible; P07664; DM.
DR GO; GO:0005829; C:cytosol; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005705; C:polytene chromosome interband; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008595; P:anterior/posterior axis specification, embryo; IMP:FlyBase.
DR GO; GO:0045450; P:bicoid mRNA localization; IMP:UniProtKB.
DR GO; GO:0048477; P:oogenesis; IGI:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0019953; P:sexual reproduction; IEP:FlyBase.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 3.
DR SMART; SM00355; ZnF_C2H2; 7.
DR SUPFAM; SSF57667; SSF57667; 3.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 6.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 6.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Developmental protein; DNA-binding; Metal-binding;
KW Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..433
FT /note="Serendipity locus protein delta"
FT /id="PRO_0000047050"
FT ZN_FING 194..217
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 223..245
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 251..273
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 279..301
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 308..330
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 337..359
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 405..428
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 141..162
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 187..193
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:1849091"
FT COMPBIAS 145..162
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 62
FT /note="Missing (in Ref. 1; CAA26898)"
FT /evidence="ECO:0000305"
FT CONFLICT 272
FT /note="M -> S (in Ref. 1; CAA26898)"
FT /evidence="ECO:0000305"
FT CONFLICT 393
FT /note="V -> F (in Ref. 1 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 413
FT /note="N -> T (in Ref. 1 and 5)"
FT /evidence="ECO:0000305"
FT CONFLICT 431..433
FT /note="VLK -> S (in Ref. 1 and 5)"
FT /evidence="ECO:0000305"
FT STRAND 2..4
FT /evidence="ECO:0007829|PDB:7POH"
FT TURN 31..33
FT /evidence="ECO:0007829|PDB:7POH"
FT HELIX 37..47
FT /evidence="ECO:0007829|PDB:7POH"
FT HELIX 62..90
FT /evidence="ECO:0007829|PDB:7POH"
SQ SEQUENCE 433 AA; 50028 MW; 9F89FB7696ECF3F7 CRC64;
MDTCFFCGAV DLSDTGSSSS MRYETLSAKV PSSQKTVSLV LTHLANCIQT QLDLKPGARL
CPRCFQELSD YDTIMVNLMT TQKRLTTQLK GALKSEFEVP ESGEDILVEE VEIPQSDVET
DADAEADALF VELVKDQEES DTEIKREFVD EEEEEDDDDD DEFICEDVDV GDSEALYGKS
SDGEDRPTKK RVKQECTTCG KVYNSWYQLQ KHISEEHSKQ PNHICPICGV IRRDEEYLEL
HMNLHEGKTE KQCRYCPKSF SRPVNTLRHM RMHWDKKKYQ CEKCGLRFSQ DNLLYNHRLR
HEAEENPIIC SICNVSFKSR KTFNHHTLIH KENRPRHYCS VCPKSFTERY TLKMHMKTHE
GDVVYGVREE APADEQQVVE ELHVDVDESE AAVTVIMSDN DENSGFCLIC NTNFENKKEL
EHHLQFDHDV VLK
