SRY_BOVIN
ID SRY_BOVIN Reviewed; 229 AA.
AC Q03255; A1XCJ6; A8WDV8;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Sex-determining region Y protein;
DE AltName: Full=Testis-determining factor;
GN Name=SRY; Synonyms=TDF;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7873883; DOI=10.1007/bf00426080;
RA Payen E.J., Cotinot C.Y.;
RT "Sequence evolution of SRY gene within Bovidae family.";
RL Mamm. Genome 5:723-725(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Daneau I., Houde A., Ethier J.-F., Lussier J.G., Silversides D.W.;
RL Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Kato Y., Sato S., Cui X., Itagaki Y., Sutou S.;
RT "Cloning and characterization of bovine SRY gene.";
RL Anim. Sci. Technol. 66:994-1000(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Nijman I.J., van Boxtel D.C.J., van Cann L.M., Cuppen E., Lenstra J.A.;
RT "Phylogeny of Y-chromosomes from interbreeding bovine species.";
RL Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Z11;
RA Gou X., Mao H.;
RT "Genetic diversity and origin of gayal and cattle in Yunnan revealed by
RT mtDNA D-loop and SRY gene sequence variation.";
RL Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Pei J.;
RL Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 61-120.
RC TISSUE=Blood;
RX PubMed=8332478; DOI=10.1093/nar/21.11.2772;
RA Payen E.J., Cotinot C.Y.;
RT "Comparative HMG-box sequences of the SRY gene between sheep, cattle and
RT goats.";
RL Nucleic Acids Res. 21:2772-2772(1993).
RN [8]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 65-113.
RC TISSUE=Liver;
RX PubMed=8199324;
RA Zhai W., Chen X., Wu D., Xu J., Gao Y., Zhu L.;
RT "Amplification and analysis of a male-specific SRY homologous sequence of
RT cattle.";
RL Chin. J. Biotechnol. 9:79-84(1993).
CC -!- FUNCTION: Transcriptional regulator that controls a genetic switch in
CC male development. It is necessary and sufficient for initiating male
CC sex determination by directing the development of supporting cell
CC precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells.
CC Involved in different aspects of gene regulation including promoter
CC activation or repression. Binds to the DNA consensus sequence 5'-
CC [AT]AACAA[AT]-3'. SRY HMG box recognizes DNA by partial intercalation
CC in the minor groove and promotes DNA bending. Also involved in pre-mRNA
CC splicing (By similarity). In male adult brain involved in the
CC maintenance of motor functions of dopaminergic neurons (By similarity).
CC {ECO:0000250|UniProtKB:P36394, ECO:0000250|UniProtKB:Q05066}.
CC -!- SUBUNIT: Interacts with CALM, EP300, HDAC3, KPNB1, ZNF208 isoform KRAB-
CC O, PARP1, SLC9A3R2 and WT1. The interaction with EP300 modulates its
CC DNA-binding activity. The interaction with KPNB1 is sensitive to
CC dissociation by Ran in the GTP-bound form. Interaction with PARP1
CC impaired its DNA-binding activity. {ECO:0000250|UniProtKB:Q05066}.
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000250|UniProtKB:Q05066}.
CC Cytoplasm {ECO:0000250|UniProtKB:Q05066}. Nucleus
CC {ECO:0000250|UniProtKB:Q05066}.
CC -!- PTM: Acetylation of Lys-130 contributes to its nuclear localization and
CC enhances its interaction with KPNB1. Deacetylated by HDAC3.
CC {ECO:0000250|UniProtKB:Q05066}.
CC -!- SIMILARITY: Belongs to the SRY family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=The tenuous nature of sex
CC - Issue 80 of March 2007;
CC URL="https://web.expasy.org/spotlight/back_issues/080";
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DR EMBL; Z30327; CAA82981.1; -; Genomic_DNA.
DR EMBL; U15569; AAA50602.1; -; Genomic_DNA.
DR EMBL; AB039748; BAA92368.1; -; Genomic_DNA.
DR EMBL; DQ336526; ABC67486.1; -; Genomic_DNA.
DR EMBL; EU233320; ABW89571.1; -; Genomic_DNA.
DR EMBL; EU294189; ABY19364.1; -; Genomic_DNA.
DR EMBL; Z18808; CAA79272.1; -; Genomic_DNA.
DR EMBL; S71489; AAD14086.1; -; Genomic_DNA.
DR PIR; S34113; S34113.
DR RefSeq; NP_001014407.1; NM_001014385.1.
DR RefSeq; XP_015321341.1; XM_015465855.1.
DR RefSeq; XP_015329440.1; XM_015473954.1.
DR AlphaFoldDB; Q03255; -.
DR SMR; Q03255; -.
DR STRING; 9913.ENSBTAP00000056194; -.
DR PaxDb; Q03255; -.
DR GeneID; 280931; -.
DR CTD; 6736; -.
DR eggNOG; KOG0527; Eukaryota.
DR HOGENOM; CLU_1209425_0_0_1; -.
DR InParanoid; Q03255; -.
DR OrthoDB; 369754at2759; -.
DR Proteomes; UP000009136; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0030238; P:male sex determination; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0010628; P:positive regulation of gene expression; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0007548; P:sex differentiation; IEA:UniProtKB-KW.
DR Gene3D; 1.10.30.10; -; 1.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR InterPro; IPR017253; SRY.
DR PANTHER; PTHR10270:SF199; PTHR10270:SF199; 1.
DR Pfam; PF00505; HMG_box; 1.
DR PIRSF; PIRSF037653; SRY; 1.
DR SMART; SM00398; HMG; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
DR PROSITE; PS50118; HMG_BOX_2; 1.
PE 3: Inferred from homology;
KW Acetylation; Activator; Calmodulin-binding; Cytoplasm; Differentiation;
KW DNA-binding; Nucleus; Reference proteome; Repressor;
KW Sexual differentiation; Transcription; Transcription regulation.
FT CHAIN 1..229
FT /note="Sex-determining region Y protein"
FT /id="PRO_0000048648"
FT DNA_BIND 54..122
FT /note="HMG box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 32..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 37..52
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 120
FT /note="G -> A (in Ref. 7; CAA79272)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 229 AA; 26627 MW; 41AA6ED61E541D24 CRC64;
MFRVLNDDVY SPAVVQQQTT LAFRKDSSLC TDSHSANDQC ERGEHVRESS QDHVKRPMNA
FIVWSRERRR KVALENPKMK NSDISKQLGY EWKRLTDAEK RPFFEEAQRL LAIHRDKYPG
YKYRPRRRAK RPQKSLPADS SILCNPMHVE TLHPFTYRDG CAKTTYSQME SQLSRSQSVI
ITNSLLQKEH HSSWTSLGHN KVTLATRISA DFPCNKSLEP GLSCAYFQY
