ID   SRY_CANLF               Reviewed;         220 AA.
AC   Q9XT60;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=Sex-determining region Y protein;
DE   AltName: Full=Testis-determining factor;
GN   Name=SRY; Synonyms=TDF;
OS   Canis lupus familiaris (Dog) (Canis familiaris).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
OX   NCBI_TaxID=9615;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10369387;
RX   DOI=10.1002/(sici)1098-2795(199907)53:3<266::aid-mrd2>3.0.co;2-6;
RA   Meyers-Wallen V.N., Schlafer D., Barr I., Lovell-Badge R., Keyzner A.;
RT   "Sry-negative XX sex reversal in purebred dogs.";
RL   Mol. Reprod. Dev. 53:266-273(1999).
CC   -!- FUNCTION: Transcriptional regulator that controls a genetic switch in
CC       male development. It is necessary and sufficient for initiating male
CC       sex determination by directing the development of supporting cell
CC       precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells.
CC       Involved in different aspects of gene regulation including promoter
CC       activation or repression. Binds to the DNA consensus sequence 5'-
CC       [AT]AACAA[AT]-3'. SRY HMG box recognizes DNA by partial intercalation
CC       in the minor groove and promotes DNA bending. Also involved in pre-mRNA
CC       splicing (By similarity). In male adult brain involved in the
CC       maintenance of motor functions of dopaminergic neurons (By similarity).
CC       {ECO:0000250|UniProtKB:P36394, ECO:0000250|UniProtKB:Q05066}.
CC   -!- SUBUNIT: Interacts with CALM, EP300, HDAC3, KPNB1, ZNF208 isoform KRAB-
CC       O, PARP1, SLC9A3R2 and WT1. The interaction with EP300 modulates its
CC       DNA-binding activity. The interaction with KPNB1 is sensitive to
CC       dissociation by Ran in the GTP-bound form. Interaction with PARP1
CC       impaired its DNA-binding activity. {ECO:0000250|UniProtKB:Q05066}.
CC   -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000250|UniProtKB:Q05066}.
CC       Cytoplasm {ECO:0000250|UniProtKB:Q05066}. Nucleus
CC       {ECO:0000250|UniProtKB:Q05066}.
CC   -!- SIMILARITY: Belongs to the SRY family. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=The tenuous nature of sex
CC       - Issue 80 of March 2007;
CC       URL="https://web.expasy.org/spotlight/back_issues/080";
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DR   EMBL; AF107021; AAD40225.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9XT60; -.
DR   SMR; Q9XT60; -.
DR   Ensembl; ENSCAFT00030015158; ENSCAFP00030013218; ENSCAFG00030008259.
DR   Ensembl; ENSCAFT00845040434; ENSCAFP00845031662; ENSCAFG00845022918.
DR   VEuPathDB; HostDB:ENSCAFG00845022918; -.
DR   GeneTree; ENSGT00940000165583; -.
DR   InParanoid; Q9XT60; -.
DR   Proteomes; UP000002254; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0140297; F:DNA-binding transcription factor binding; IEA:Ensembl.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR   GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR   GO; GO:0030238; P:male sex determination; IBA:GO_Central.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0010628; P:positive regulation of gene expression; ISS:UniProtKB.
DR   GO; GO:2000020; P:positive regulation of male gonad development; IEA:Ensembl.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   GO; GO:0007548; P:sex differentiation; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.30.10; -; 1.
DR   InterPro; IPR009071; HMG_box_dom.
DR   InterPro; IPR036910; HMG_box_dom_sf.
DR   InterPro; IPR017253; SRY.
DR   PANTHER; PTHR10270:SF199; PTHR10270:SF199; 1.
DR   Pfam; PF00505; HMG_box; 1.
DR   PIRSF; PIRSF037653; SRY; 1.
DR   SMART; SM00398; HMG; 1.
DR   SUPFAM; SSF47095; SSF47095; 1.
DR   PROSITE; PS50118; HMG_BOX_2; 1.
PE   3: Inferred from homology;
KW   Activator; Calmodulin-binding; Cytoplasm; Differentiation; DNA-binding;
KW   Nucleus; Reference proteome; Sexual differentiation; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..220
FT                   /note="Sex-determining region Y protein"
FT                   /id="PRO_0000048652"
FT   DNA_BIND        53..121
FT                   /note="HMG box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT   REGION          25..52
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        25..42
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   220 AA;  25505 MW;  7AA1A474FBA4C28E CRC64;
     MFRALNCDDH GAAVQQNAFG FPRKSSDRWT DNSTSNYRCE SGGNGRDSGR NRVRRPMNAF
     LVWSRDQRRK MALENPQMQN SEISKQLGYQ WKMLTEAEKW PFFEEAQRLQ AMHREKYPDY
     KYRPRRKATA QKSHKLLPAA SSSMLCKQVH VDERFYPFTY TDSCSRAAHT RMEDQLSCSQ
     PMSTARSLLQ QEYHSSSASL RDSPETLAAQ MSADASFYSK