SS120_YEAST
ID SS120_YEAST Reviewed; 234 AA.
AC P39931; D6VYP8;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Protein SSP120;
DE Flags: Precursor;
GN Name=SSP120; OrderedLocusNames=YLR250W; ORFNames=L9672.4;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1743509; DOI=10.1016/0378-1119(91)90303-s;
RA Sidhu R.S., Mathewes S., Bollon A.P.;
RT "Selection of secretory protein-encoding genes by fusion with PHO5 in
RT Saccharomyces cerevisiae.";
RL Gene 107:111-118(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-212, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- MISCELLANEOUS: Present with 6900 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; M59382; AAA35068.1; -; Genomic_DNA.
DR EMBL; U20865; AAB67390.1; -; Genomic_DNA.
DR EMBL; AY558213; AAS56539.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09564.1; -; Genomic_DNA.
DR PIR; JH0483; JH0483.
DR RefSeq; NP_013351.1; NM_001182137.1.
DR AlphaFoldDB; P39931; -.
DR BioGRID; 31518; 106.
DR DIP; DIP-5286N; -.
DR IntAct; P39931; 2.
DR STRING; 4932.YLR250W; -.
DR iPTMnet; P39931; -.
DR MaxQB; P39931; -.
DR PaxDb; P39931; -.
DR PRIDE; P39931; -.
DR EnsemblFungi; YLR250W_mRNA; YLR250W; YLR250W.
DR GeneID; 850952; -.
DR KEGG; sce:YLR250W; -.
DR SGD; S000004240; SSP120.
DR VEuPathDB; FungiDB:YLR250W; -.
DR eggNOG; ENOG502QRFY; Eukaryota.
DR GeneTree; ENSGT00390000001927; -.
DR HOGENOM; CLU_096561_1_0_1; -.
DR InParanoid; P39931; -.
DR OMA; EHFRKHD; -.
DR BioCyc; YEAST:G3O-32355-MON; -.
DR PRO; PR:P39931; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; P39931; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005793; C:endoplasmic reticulum-Golgi intermediate compartment; IBA:GO_Central.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR040250; Nucleobindin.
DR PANTHER; PTHR19237; PTHR19237; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome; Repeat; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..234
FT /note="Protein SSP120"
FT /id="PRO_0000022420"
FT DOMAIN 52..87
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 108..143
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT MOD_RES 212
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:18407956"
SQ SEQUENCE 234 AA; 27289 MW; 50BE3035F6B45769 CRC64;
MRFLRGFVFS LAFTLYKVTA TAEIGSEINV ENEAPPDGLS WEEWHMDHEH QLKDYTPETF
FALHDIKKKG FLDENDILSL YGLNREEIVG AGDGMGQHDE SEKIDNEMAK RVVSLIMRLL
DVDDNTKITK EEYLQFAKRG NKFPDLGVGV GHHSDFELEY EIHHWNKFHK DKDPDVKVVH
KEDIEHELLH HEHEIEHEEE IQRGASRATV ITDDELESRI ELKNIPEKFK NGIF
