ID   SSA1_MANHA              Reviewed;         932 AA.
AC   P31631;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=Serotype-specific antigen 1;
DE            EC=3.4.21.-;
DE   Flags: Precursor;
GN   Name=ssa1;
OS   Mannheimia haemolytica (Pasteurella haemolytica).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Mannheimia.
OX   NCBI_TaxID=75985;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 58-64.
RC   STRAIN=Serotype A1;
RX   PubMed=1840576; DOI=10.1128/iai.59.10.3398-3406.1991;
RA   Lo R.Y.C., Strathdee C.A., Shewen P.E., Cooney B.J.;
RT   "Molecular studies of Ssa1, a serotype-specific antigen of Pasteurella
RT   haemolytica A1.";
RL   Infect. Immun. 59:3398-3406(1991).
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane.
CC   -!- SIMILARITY: Belongs to the peptidase S8 family. {ECO:0000305}.
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DR   EMBL; M62363; AAA25549.1; -; Genomic_DNA.
DR   PIR; A43608; SWQPA1.
DR   AlphaFoldDB; P31631; -.
DR   SMR; P31631; -.
DR   STRING; 75985.WC39_09550; -.
DR   MEROPS; S08.144; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   CDD; cd04848; Peptidases_S8_Autotransporter_serine_protease_like; 1.
DR   Gene3D; 2.40.128.130; -; 1.
DR   Gene3D; 3.40.50.200; -; 1.
DR   InterPro; IPR005546; Autotransporte_beta.
DR   InterPro; IPR036709; Autotransporte_beta_dom_sf.
DR   InterPro; IPR000209; Peptidase_S8/S53_dom.
DR   InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR   InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR   InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR   InterPro; IPR034061; Peptidases_S8_Autotransporter.
DR   Pfam; PF03797; Autotransporter; 1.
DR   Pfam; PF00082; Peptidase_S8; 1.
DR   PRINTS; PR00723; SUBTILISIN.
DR   SMART; SM00869; Autotransporter; 1.
DR   SUPFAM; SSF103515; SSF103515; 1.
DR   SUPFAM; SSF52743; SSF52743; 1.
DR   PROSITE; PS51208; AUTOTRANSPORTER; 1.
DR   PROSITE; PS51892; SUBTILASE; 1.
DR   PROSITE; PS00138; SUBTILASE_SER; 1.
PE   1: Evidence at protein level;
KW   Cell outer membrane; Direct protein sequencing; Hydrolase; Membrane;
KW   Protease; Serine protease; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..932
FT                   /note="Serotype-specific antigen 1"
FT                   /id="PRO_0000027167"
FT   DOMAIN          25..407
FT                   /note="Peptidase S8"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   DOMAIN          669..932
FT                   /note="Autotransporter"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00556"
FT   ACT_SITE        58
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        116
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        351
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01240"
FT   CONFLICT        62
FT                   /note="M -> V (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   932 AA;  103590 MW;  15F2DEDF9DFB5678 CRC64;
     MYKIKHSFNK TLIAISISSF LSIAYATESI ENPQPIIQLS ESLSSKYSGK GVKLGVMDEG
     FMVKHPRHSS HLHPLIHQLT TPEGEVRIYD ASYPQFEVNP VEKEDGIDLI PSLETHGAGV
     AGIIAAQADK TLGDGYSGGI AKGAELYVAT KSYKRTLEKV IQDAKKELEN AKDEEDEKTP
     SLDQMAKNDL LASKEKEMAI ERAEWASGLN KLLDNNVFAI NNSWNPFSIS DDINVVDKFY
     QSIKQNKHNP LLQAIMRAKN SNTLLVFAAG NESKKQPGVM ALLPRYFPEL EKNLISAVAV
     DKEQKIASYS NHCGASKNWC VAAPGDLHVL IGVADEHKKP QYGLTKEQGT SFSAPAITAS
     LAVLKERFDY LTATQIRDTL LTTATDLGEK GVDNVYGWGL INLKKAVNGP TQFLNDETIT
     VTRDDHWSNP LASQFKITKK GDKSLHLDGE NHLDTVAVEE GRLALNGKTK VKTISNHANL
     AVNGTEVEQN YSSSGQSQLE VLGKSGLIAN AQANIHLAGS LKIDDKLTEK TEAGDVSATV
     VQLKDKATYQ GGFTQLVENE NLAKRGLIQD LYFKESEIIA KVNKPLTDEK ADTNGQAGLA
     LLNALRTTPI AYRRSWYNGW LQSALEQRKL DNLHYAVSNN IYADSLELLR SQNRKGLTQA
     QQHLFTAYHT PLQTTVWAEH LNQKQSASSK HTDVKHHQSQ LGVNHKLADK TVLSATLSQQ
     KNRLEKPFAQ ATLKQTALNI GLRYHLDNAW FSEATLQFAR QKYQQSRRFA SHQLGTAETR
     GSTLGGEMRI GYQFMPNQWI IEPSLGVQWI QTKMNGLNES GELATQTAAM RYRDVNIVPS
     VKLQRTFQLE QGSISPYIGL NYLHRLNGKI TKITSNIAGK TLHSEATTKR NRQLNGEVGV
     KLHYKNWFTA MNLDYSRVKS CKPIWLESKC WL