ID   SSAJ_SALTY              Reviewed;         249 AA.
AC   P74852;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Secretion system apparatus lipoprotein SsaJ;
DE   Flags: Precursor;
GN   Name=ssaJ; OrderedLocusNames=STM1409;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LT2;
RX   PubMed=9140973; DOI=10.1046/j.1365-2958.1997.3271699.x;
RA   Hensel M., Shea J.E., Raupach B., Monack D., Falkow S., Gleeson C.,
RA   Kubo T., Holden D.W.;
RT   "Functional analysis of ssaJ and the ssaK/U operon, 13 genes encoding
RT   components of the type III secretion apparatus of Salmonella pathogenicity
RT   island 2.";
RL   Mol. Microbiol. 24:155-167(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
RN   [3]
RP   ROLE IN SPVB EXPORT, AND DISRUPTION PHENOTYPE.
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=18248436; DOI=10.1111/j.1574-695x.2007.00364.x;
RA   Browne S.H., Hasegawa P., Okamoto S., Fierer J., Guiney D.G.;
RT   "Identification of Salmonella SPI-2 secretion system components required
RT   for SpvB-mediated cytotoxicity in macrophages and virulence in mice.";
RL   FEMS Immunol. Med. Microbiol. 52:194-201(2008).
CC   -!- FUNCTION: Component of Salmonella pathogenicity island 2 (SPI-2) type
CC       III secretion system, required for secretion of some type III-secreted
CC       effectors including the SpvB toxin. {ECO:0000269|PubMed:18248436}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000305}.
CC   -!- DISRUPTION PHENOTYPE: Disruption prevents SpvB-induced F-actin
CC       depolymerization in human macrophages without affecting intra-bacterial
CC       SpvB protein levels. {ECO:0000269|PubMed:18248436}.
CC   -!- SIMILARITY: Belongs to the YscJ lipoprotein family. {ECO:0000305}.
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DR   EMBL; Y09357; CAA70532.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL20333.1; -; Genomic_DNA.
DR   RefSeq; NP_460374.1; NC_003197.2.
DR   RefSeq; WP_000862874.1; NC_003197.2.
DR   AlphaFoldDB; P74852; -.
DR   SMR; P74852; -.
DR   STRING; 99287.STM1409; -.
DR   PaxDb; P74852; -.
DR   EnsemblBacteria; AAL20333; AAL20333; STM1409.
DR   GeneID; 1252927; -.
DR   KEGG; stm:STM1409; -.
DR   PATRIC; fig|99287.12.peg.1493; -.
DR   HOGENOM; CLU_073268_1_0_6; -.
DR   OMA; GRIQQMV; -.
DR   PhylomeDB; P74852; -.
DR   BioCyc; SENT99287:STM1409-MON; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR   Gene3D; 3.30.300.30; -; 1.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR006182; FliF_N_dom.
DR   InterPro; IPR003282; T3SS_HrcJ/YscJ.
DR   InterPro; IPR043427; YscJ/FliF.
DR   PANTHER; PTHR30046; PTHR30046; 1.
DR   Pfam; PF01514; YscJ_FliF; 1.
DR   PRINTS; PR01338; TYPE3OMKPROT.
DR   TIGRFAMs; TIGR02544; III_secr_YscJ; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Lipoprotein; Membrane; Palmitate; Protein transport;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix; Transport.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           19..249
FT                   /note="Secretion system apparatus lipoprotein SsaJ"
FT                   /id="PRO_0000018229"
FT   TRANSMEM        225..245
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   LIPID           19
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           19
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   249 AA;  28521 MW;  A6999389900AA641 CRC64;
     MKVHRIVFLT VLTFFLTACD VDLYRSLPED EANQMLALLM QHHIDAEKKQ EEDGVTLRVE
     QSQFINAVEL LRLNGYPHRQ FTTADKMFPA NQLVVSPQEE QQKINFLKEQ RIEGMLSQME
     GVINAKVTIA LPTYDEGSNA SPSSVAVFIK YSPQVNMEAF RVKIKDLIEM SIPGLQYSKI
     SILMQPAEFR MVADVPARQT FWIMDVINAN KGKVVKWLMK YPYPLMLSLT GLLLGVGILI
     GYFCLRRRF